Kit for detecting susceptibility of hebephrenic schizophrenia

Abstract

The invention discloses a kit for detecting susceptibility genes of hebephrenic schizophrenia. The method for detecting susceptibility genes of hebephrenic schizophrenia, provided by the invention, refers to a polymerase chain reaction-restriction fragment length polymorphism analysis method. A polymorphic site, rs1800497, of a gene sequence of susceptibility genes DRD2 is detected by the method. The kit disclosed by the invention can be used for accurately detecting the susceptibility genes of hebephrenic schizophrenia and provides a new thought for thorough research, prevention and treatment, diagnosis and treatment of the hebephrenic schizophrenia.

Description

technical field

[0001] The invention relates to the field of detection of schizophrenia susceptibility genes, in particular to a kit for detecting susceptibility to adolescent schizophrenia. Background technique

[0002] Schizophrenia is a chronic, severe, and disabling encephalopathy that seriously endangers human health. Schizophrenia is the most serious mental illness among mental illnesses. According to the Lancet survey in 2009, the number of schizophrenia patients in my country is nearly 10 million. Schizophrenia requires lifelong treatment, and the cost of treatment is high. According to statistics, the total cost of schizophrenia treatment in the United States in 2002 reached 62.7 billion US dollars, of which 22.7 billion US dollars were direct costs, premature deaths due to suicide and losses caused by family members taking care of patients etc. is 32.4 billion US dollars, and schizophrenia is highly disabling. Most patients with schizophrenia will have some residu...

Examples

Embodiment 1

[0043] 1. Select candidate genes and SNPs

[0044] The present inventor consults literature, utilizes computer Internet and bioinformatics to obtain various aspects of schizophrenia candidate gene research, and carries out single nucleotide polymorphism (SNP) linkage disequilibrium analysis by linkage disequilibrium analysis method on No. 11 chromosome, through NCBI (http: / / www.ncbi.nlm.nih.gov / mapviewer) database, access to DRD2 gene, and through http: / / www.ncbi.nlm.nih.gov / SNP, http: / / snp.cshl. org / database to search for candidate gene SNPs that meet its conditions. The selection criteria: 1. There are relevant literature reports; 2. The minimum allele frequency of the site should be greater than 10%; 3. The selected SNPs must meet the requirements of the primer design software; 4. The selected site cannot affect the gene Typing experiment

[0045] 2. Materials and methods

[0046] 2.1 Research object

[0047] The present invention takes 320 cases of juvenile schizophre...

Embodiment 2

[0088] Validation test: Using this kit, randomly select 20 samples of adolescent schizophrenia patients and 20 samples of the control group, and detect the polymorphism of DRD2 gene rs1800497 by PCR sequencing.

[0089] 1. PCR amplification:

[0090] The PCR amplification conditions are: 1. 94°C for 5min, 2. 96°C for 45s, 60°C for 60s, 72°C for 60s for 3 cycles, 3. 95°C for 45s, 59°C for 65s, 72°C for 60s for 5 cycles, 4. 94°C for 45s, 57°C for 60s, 72°C for 60s, 32 cycles, 5, 72°C for 10min, take 5uL of PCR product and electrophoresis on 1.5% agarose gel, using DNAmarker as the molecular weight standard.

[0091] 2. Identification of genotype by restriction endonuclease digestion

[0092] The enzyme digestion reaction system is 20uL, including 10uL PCR product, 0.5uL restriction endonuclease TaqI, 2uL enzyme digestion buffer, placed in a 37°C incubator for 4 hours, and subjected to 3% agarose gel electrophoresis to determine the gene according to the enzyme digestion map ty...

technical field

[0001] The invention relates to the field of detection of schizophrenia susceptibility genes, in particular to a kit for detecting susceptibility to adolescent schizophrenia. Background technique

[0002] Schizophrenia is a chronic, severe, and disabling encephalopathy that seriously endangers human health. Schizophrenia is the most serious mental illness among mental illnesses. According to the Lancet survey in 2009, the number of schizophrenia patients in my country is nearly 10 million. Schizophrenia requires lifelong treatment, and the cost of treatment is high. According to statistics, the total cost of schizophrenia treatment in the United States in 2002 reached 62.7 billion US dollars, of which 22.7 billion US dollars were direct costs, premature deaths due to suicide and losses caused by family members taking care of patients etc. is 32.4 billion US dollars, and schizophrenia is highly disabling. Most patients with schizophrenia will have some residu...

Examples

Embodiment 1

[0043] 1. Select candidate genes and SNPs

[0044] The present inventor consults literature, utilizes computer Internet and bioinformatics to obtain various aspects of schizophrenia candidate gene research, and carries out single nucleotide polymorphism (SNP) linkage disequilibrium analysis by linkage disequilibrium analysis method on No. 11 chromosome, through NCBI (http: / / www.ncbi.nlm.nih.gov / mapviewer) database, access to DRD2 gene, and through http: / / www.ncbi.nlm.nih.gov / SNP, http: / / snp.cshl. org / database to search for candidate gene SNPs that meet its conditions. The selection criteria: 1. There are relevant literature reports; 2. The minimum allele frequency of the site should be greater than 10%; 3. The selected SNPs must meet the requirements of the primer design software; 4. The selected site cannot affect the gene Typing experiment

[0045] 2. Materials and methods

[0046] 2.1 Research object

[0047] The present invention takes 320 cases of juvenile schizophre...

Embodiment 2

[0088] Validation test: Using this kit, randomly select 20 samples of adolescent schizophrenia patients and 20 samples of the control group, and detect the polymorphism of DRD2 gene rs1800497 by PCR sequencing.

[0089] 1. PCR amplification:

[0090] The PCR amplification conditions are: 1. 94°C for 5min, 2. 96°C for 45s, 60°C for 60s, 72°C for 60s for 3 cycles, 3. 95°C for 45s, 59°C for 65s, 72°C for 60s for 5 cycles, 4. 94°C for 45s, 57°C for 60s, 72°C for 60s, 32 cycles, 5, 72°C for 10min, take 5uL of PCR product and electrophoresis on 1.5% agarose gel, using DNAmarker as the molecular weight standard.

[0091] 2. Identification of genotype by restriction endonuclease digestion

[0092] The enzyme digestion reaction system is 20uL, including 10uL PCR product, 0.5uL restriction endonuclease TaqI, 2uL enzyme digestion buffer, placed in a 37°C incubator for 4 hours, and subjected to 3% agarose gel electrophoresis to determine the gene according to the enzyme digestion map ty...