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Primer and kit for detecting susceptibility of paranoia schizophrenia

A technology of schizophrenia and kit, which is applied in the field of schizophrenia susceptibility gene detection, to achieve the effect of fast process

Inactive Publication Date: 2016-05-04
QINGDAO TUMOR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research result on the correlation between GNB1L gene and paranoid schizophrenia. For the first time, this invention uses SNP technology to detect the susceptibility genes of paranoid schizophrenia, which is an in-depth study and prevention of paranoid schizophrenia. , diagnosis, and treatment provide new ideas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Select candidate genes and SNPs

[0049] The present inventor consults literature, utilizes computer Internet and bioinformatics to obtain various aspects of schizophrenia candidate gene research, and carries out single nucleotide polymorphism (SNP) linkage disequilibrium analysis by linkage disequilibrium analysis method on No. 22 chromosome, through NCBI (http: / / www.ncbi.nlm.nih.gov / mapviewer) database, access to GNB1L gene, and through http: / / www.ncbi.nlm.nih.gov / SNP, http: / / snp.cshl. org / database to search for candidate gene SNPs that meet its conditions. The selection criteria: 1. There are relevant literature reports; 2. The minimum allele frequency of the site should be greater than 10%; 3. The selected SNPs must meet the requirements of the primer design software; 4. The selected site cannot affect the gene Typing experiment

[0050] 2. Research object

[0051] The present invention takes 451 cases of paranoid schizophrenia and 460 cases of healthy contro...

Embodiment 2

[0094] Validation test: Using this kit, randomly select 20 samples from patients with paranoid schizophrenia and 20 samples from the control group, and detect the polymorphism at rs748806 of the GNB1L gene by PCR sequencing.

[0095] 1. PCR amplification:

[0096] Amplify the partial fragment of GNB1L gene rs748806 by PCR. The PCR reaction system is: 3 μL of 10×PCR reaction buffer, 0.5 μL of 10 mM / LdNTP, 0.5 μL of TaqDNA polymerase, 0.5 μL of 10pM / L primer, 1 μL of genomic DNA, and add deionized water to 30 μL. During PCR, 20 μL of paraffin oil was added to each system to prevent the liquid from volatilizing.

[0097] The PCR reaction conditions were pre-denaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 25 s, a total of 35 cycles, and a total extension at 72°C for 2 min.

[0098] 2. Sequencing to determine genotype

[0099] The PCR product was detected by 8% polyacrylamide gel electrophoresis, and the gel imagin...

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Abstract

The invention discloses a primer and a kit for detecting the susceptibility of paranoia schizophrenia. A method for detecting susceptibility genes of the paranoia schizophrenia relates to the detection the nucleotide sequence of a susceptibility gene GNB1L and particularly relates to an application of the primer and kit by virtue of detection of a polymorphic site rs748806 of the gene sequence of the susceptibility gene GNB1L in early evaluation of the paranoia schizophrenia. By virtue of the primer and the kit, the susceptibility gene of the paranoia schizophrenia can be accurately detected, and a new thought is provided for the deep research, the auxiliary diagnosis, the prevention, the treatment and the new drug research and development of the paranoia schizophrenia.

Description

technical field [0001] The invention relates to the field of detection of schizophrenia susceptibility genes, in particular to a primer and a kit for detecting the susceptibility of paranoid schizophrenia. Background technique [0002] Schizophrenia is a chronic, severe, and disabling encephalopathy that seriously endangers human health. Schizophrenia is the most serious mental illness among mental illnesses. According to the Lancet survey in 2009, the number of schizophrenia patients in my country is nearly 10 million. Schizophrenia requires lifelong treatment, and the cost of treatment is high. According to statistics, the total cost of schizophrenia treatment in the United States in 2002 reached 62.7 billion US dollars, of which 22.7 billion US dollars were direct costs, premature deaths due to suicide and losses caused by family members taking care of patients etc. is 32.4 billion US dollars, and schizophrenia is highly disabling. Most patients with schizophrenia will h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/112C12Q2600/156
Inventor 张海茂李莉邓华
Owner QINGDAO TUMOR HOSPITAL
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