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A kind of fingerprint spectrum of salvianolic acid extract and content determination method of related components

A technology of salvia polyphenolic acid and fingerprint spectrum is applied in the detection field of traditional Chinese medicine extracts, which can solve the problems of lack of integrity, insufficient identification of complex components, and inaccurate quantification.

Active Publication Date: 2019-12-31
TIANJIN TASLY ZHIJIAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses salvianolic acid B as the reference to calculate the content of polyphenolic acid, and the specificity and accuracy are poor.
Moreover, for traditional Chinese medicine preparations with complex components, only one component is used to control the content index. While lacking integrity, the identification of complex components is not sufficient
[0006] Therefore, in view of the defects of insufficient identification and inaccurate quantification of the above-mentioned complex components,

Method used

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  • A kind of fingerprint spectrum of salvianolic acid extract and content determination method of related components
  • A kind of fingerprint spectrum of salvianolic acid extract and content determination method of related components
  • A kind of fingerprint spectrum of salvianolic acid extract and content determination method of related components

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] Step 1: Establish a digital quantitative fingerprint of salvianolic acid extract by ultra-high performance liquid phase multi-wavelength visible ultraviolet detection method;

[0151] (1) Instruments and reagents: Waters UPLC system, Xevo G2qTOF mass spectrometer connected in series. Salvianolic acid extract was provided by Tasly Zhijiao Pharmaceutical Co., Ltd. Acetonitrile and methanol were chromatographically pure (Merck), formic acid was chromatographically pure (sigma), and ultrapure water. The reference substances Danshensu Sodium, Protocatechualdehyde, Salvianolic Acid B, and Rosmarinic Acid were all purchased from China National Institutes for Food and Drug Control, Shikoninic Acid (Tianjin Mark Biotechnology), Salvianolic Acid D (Tianjin Yifang Technology Co., Ltd.) , Salvianolic acid A (Nantong Feiyu), salvianolic acid-LM (manufactured by Tasly).

[0152] (2) Preparation of reference substance solution:

[0153] Weigh about 5 mg of the reference substance o...

Embodiment 2

[0193] Step 1: Establish a digital quantitative fingerprint of salvianolic acid extract by ultra-high performance liquid phase multi-wavelength visible ultraviolet detection method;

[0194] (1) Preparation of reference substance solution:

[0195] Weigh about 2.5 mg of the reference substance of salvianolic acid B; about 1.5 mg of each of the reference substances of rosmarinic acid and shikonin; About 1mg of each LM reference substance was placed in a 5mL volumetric flask, made up to volume with ultrapure water, shaken well, passed through a microporous membrane (0.22μm), and the filtrate was obtained.

[0196] (2) Preparation of the test solution: 10 batches of salvianolic acid extracts were accurately weighed respectively, the samples were dissolved in ultrapure water, fixedly dissolved in a 10ml volumetric flask, passed through a 0.22 μm filter membrane after dissolving, and diluted to a concentration of 5mg / mL solution;

[0197] (3) Determination of fingerprints: Accura...

Embodiment 3

[0227] Step 1: Establish a digital quantitative fingerprint of salvianolic acid extract by ultra-high performance liquid phase multi-wavelength visible ultraviolet detection method;

[0228] (1) Preparation of reference substance solution:

[0229] Weigh about 7.5 mg of the reference substance of salvianolic acid B; about 2 mg of the reference substance of rosmarinic acid and shikoninic acid; About 2.5mg each of the reference substances was placed in a 5mL volumetric flask, made up to volume with ultrapure water, shaken well, passed through a microporous membrane (0.22μm), and the filtrate was obtained.

[0230] (2) Preparation of the test solution: 50 batches of salvianolic acid extracts were accurately weighed respectively, the samples were dissolved in ultrapure water, fixedly dissolved in a 10ml volumetric flask, passed through a 0.22 μm filter membrane after dissolving, and diluted to a concentration of 3mg / mL solution;

[0231] (3) Determination of fingerprint spectrum...

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Abstract

The invention relates to establishment of a salvianolic acid extract fingerprint spectrum and a content measurement method of related components. The content measurement method includes the following steps: 1) establishing the salvianolic acid extract digitized quantitative fingerprint spectrum through ultrahigh performance liquid multi-wavelength visible ultraviolet detection method; 2) recognizing peaks in the fingerprint spectrum in the step 1) with mass spectrum to determine common fingerprint peaks of ultraviolet detection; and 3) performing measurement to contents of three indicative components, which are high in content, significant in activity and definite in structure, recognized in the step 2) through high performance liquid chromatography variable-wavelength detection method.

Description

Technical field: [0001] The present invention relates to a detection method for extracts of traditional Chinese medicines, in particular to a method for establishing fingerprints of salvianolic acid extracts and determining the content of related components Background technique: [0002] Salvia miltiorrhiza is a plant of the genus Salvia in the family Lamiaceae. Also known as: Purple Salvia, Red Root, Blood Ginseng, Dahongpao, etc. The root of Danshen is used for medicinal purposes, it tastes bitter and slightly cold in nature. It has the functions of promoting blood circulation and regulating menstruation, removing blood stasis and promoting regeneration, calming the nerves, cooling blood and eliminating carbuncle, reducing swelling and relieving pain. It is used for the treatment of irregular menstruation, dysmenorrhea, postpartum stasis abdominal pain, joint pain, neurasthenia, insomnia, palpitation, carbuncle and sore. Modern medicine clinically proves that Salvia mil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N27/64
Inventor 佟玲徐静瑶刘小琳岳洪水鞠爱春
Owner TIANJIN TASLY ZHIJIAO PHARMA
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