A method for determining the fingerprint and index component content of polygala medicinal material

Abstract

The invention relates to a radix polygalae medicinal materials fingerprint chromatogram and a method for measuring content of index components. The method comprises the following steps of S1, using an ultra performance liquid chromatography full-wavelength visible ultraviolet detection method to establish the radix polygalae medicinal materials fingerprint chromatogram; S2, using a liquid chromatography-mass spectrometry technology to verify a chromatographic peak in the fingerprint chromatogram in step S1, and verifying the common finger peak and non-common peak in the ultraviolet detection; S3, using the ultra performance liquid chromatography full-wavelength visible ultraviolet detection method to measure the contents of ten index components with activities of neuroprotection, calming and mind tranquilizing, depression resisting or senile dementia resisting, and clear structures.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis and content determination of traditional Chinese medicinal materials, and specifically relates to a method for determining the fingerprint of Polygala medicinal materials and the content of index components. Background technique [0002] Polygala tenuifolia Willd., first recorded in "Shen Nong's Materia Medica", is listed as the top grade, and is regarded as a vital medicine. It is warm in nature, bitter and pungent in taste, and has the effects of soothing the nerves, improving the mind, eliminating phlegm and reducing swelling. Modern research has found that the main active ingredients of Polygala medicinal materials include saponins, sugar esters, kouzanones and alkaloids, etc., which have antibacterial, anti-inflammatory, improve cognition, improve memory, anti-senile dementia, and anti-depression , Anti-tumor, anti-aging and other pharmacological effects. [0003] The efficacy ...

AI Technical Summary

Problems solved by technology

At present, the qualitative analysis of Polygala medicinal materials is mainly fingerprint analysis, but the method established before takes a long time, consumes more reagents, is not conducive to high-throughput analysis, and is not sufficient for the identification of complex components; Quantitative analysis of components mainly focuses on the determination of individual components as indicators. It is difficult for a single index component to accurately and comprehensively reflect the internal quality of Chinese medicinal materials. The existence of problems such as difficulty in separation of reference substances, unstable monomers, difficulty in supply or high supply price makes it unable to meet the needs of quality control of traditional Chinese medicine and research and development of new drugs.

Therefore, aiming at the shortcomings of insufficient identification of the above-mentioned complex components and fewer quantitative chemical components, the present invention provides a method for determining the fingerprints of Polygala medicinal materials and the content of index components, which can more comprehensively characterize and quantify the compounds in Polygala medicinal materials analyze

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