A method for determining the fingerprint and index component content of polygala medicinal material

A technology of fingerprint and medicinal materials, which is applied in the field of fingerprint of Yuanzhi medicinal materials and the determination of the content of index components, can solve the problems of unstable monomers, difficult separation of traditional Chinese medicine reference substances, and long time consumption, and achieves the effect of shortening the measurement time.

Active Publication Date: 2018-08-24
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the qualitative analysis of Polygala medicinal materials is mainly fingerprint analysis, but the method established before takes a long time, consumes more reagents, is not conducive to high-throughput analysis, and is not sufficient for the identification of complex components; Quantitative analysis of components mainly focuses on the determination of individual components as indicators. It is difficult for a single index component to accurately and comprehensively reflect the internal quality of Chinese medicinal materials. The existence of problems such as difficulty in separation of reference substances, unstable monomers, difficulty in supply or high supply price makes it unable to meet the needs of quality control of traditional Chinese medicine and research and development of new drugs.
Therefore, aiming at the shortcomings of insufficient identification of the above-mentioned complex components and fewer quantitative chemical components, the present invention provides a method for determining the fingerprints of Polygala medicinal materials and the content of index components, which can more comprehensively characterize and quantify the compounds in Polygala medicinal materials analyze

Method used

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  • A method for determining the fingerprint and index component content of polygala medicinal material
  • A method for determining the fingerprint and index component content of polygala medicinal material
  • A method for determining the fingerprint and index component content of polygala medicinal material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step 1: Use the ultra-high performance liquid phase full-wavelength visible-ultraviolet detection method to establish the fingerprint of Polygala medicinal material; 1. Test material

[0035] Materials, instruments and reagents

[0036] Test materials: The samples of Polygala medicinal materials used were 7 batches of the whole roots of Polygala medicinal materials collected from different places of production in Shanxi Province. The whole roots of Polygala medicinal materials were collected by hand rubbing, and the wooden heart was extracted to obtain Polygala tubers, crushed, and passed through the No. 3 sieve of the Pharmacopoeia for use. See Table 1 for specific information of test materials.

[0037] Table 1 Polygala sample information table

[0038]

[0039] Apparatus: Ultra high performance liquid chromatography system (Waters company ACQUITY UPLC, including vacuum degasser, binary gradient pump, autosampler, column thermostat, TUV detector), electronic balance (CPA225D,...

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Abstract

The invention relates to a radix polygalae medicinal materials fingerprint chromatogram and a method for measuring content of index components. The method comprises the following steps of S1, using an ultra performance liquid chromatography full-wavelength visible ultraviolet detection method to establish the radix polygalae medicinal materials fingerprint chromatogram; S2, using a liquid chromatography-mass spectrometry technology to verify a chromatographic peak in the fingerprint chromatogram in step S1, and verifying the common finger peak and non-common peak in the ultraviolet detection; S3, using the ultra performance liquid chromatography full-wavelength visible ultraviolet detection method to measure the contents of ten index components with activities of neuroprotection, calming and mind tranquilizing, depression resisting or senile dementia resisting, and clear structures.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis and content determination of traditional Chinese medicinal materials, and specifically relates to a method for determining the fingerprint of Polygala medicinal materials and the content of index components. Background technique [0002] Polygala tenuifolia Willd., first recorded in "Shen Nong's Materia Medica", is listed as the top grade, and is regarded as a vital medicine. It is warm in nature, bitter and pungent in taste, and has the effects of soothing the nerves, improving the mind, eliminating phlegm and reducing swelling. Modern research has found that the main active ingredients of Polygala medicinal materials include saponins, sugar esters, kouzanones and alkaloids, etc., which have antibacterial, anti-inflammatory, improve cognition, improve memory, anti-senile dementia, and anti-depression , Anti-tumor, anti-aging and other pharmacological effects. [0003] The efficacy ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 张福生王丹丹闫艳秦雪梅张丽增
Owner SHANXI UNIV
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