Method for mutagenizing flax seeds and screening salt-tolerance mutants

Abstract

The invention relates to a method for mutagenizing flax seeds and screening salt-tolerance mutants. The method comprises the steps that firstly, 0.2-0.8 wt% of an ethylmethane sulfonate solution is used for carrying out steeping mutagenizing treatment on flax seeds, treatment is carried out for 4-16 h at the room temperature, and the mutagenized flax seeds are obtained; secondly, the mutagenized flax seeds are sown in a pot filled with gravel, culture is carried out under normal illumination and at the room temperature, a PNS nutrient solution with the concentration of NaCl ranging from 50 mmol/L to 200 mmol/L is used for irrigation everyday, and the growth condition is observed for two continuous weeks; finally, plants in a good growth state are screened-out M1 generation salt-tolerance plants. The method for culturing and screening the salt-tolerance mutants is beneficial to obtaining new salt-tolerance varieties in a later stage; a gravel culture method is adopted, the PNS nutrient solution with different concentrations of NaCl is poured regularly, and convenience is provided for subsequent result analysis; the method of ethylmethane sulfonate mutagenizing and the method of oriented screening of salt-tolerance mutant plants are combined, use is convenient, and the screening efficiency is high.

Description

technical field [0001] The invention relates to the technical field of treatment for obtaining new varieties by mutagenesis, in particular to a method for mutagenizing flax seeds and screening salt-tolerant mutants. Background technique [0002] Saline-alkali land is widely distributed in my country, accounting for 20% of the country's cultivated land, mostly in the northwest region and coastal cities. The large area of ​​saline land has seriously affected agricultural production and ecological environment. By cultivating and screening salt-tolerant economic plants and Improvement of waterlogged soil is one of the ways of soil reclamation. Therefore, cultivating and screening mutant plants to obtain new varieties has received extensive attention. [0003] The development and utilization value of flax is very high, and the fiber made from flax stem is an important raw material for the textile industry; the oil content of flax seeds is 30%-45%, and flax oil is rich in unsatura...

AI Technical Summary

Problems solved by technology

It is used to solve the problems of rapid mutagenesis of flax

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