Method for establishing rhesus monkey model with chronic process Parkinson's disease

A technology for Parkinson's disease and method establishment, applied in the field of life sciences, can solve problems such as uneven behavioral scoring standards, different emphases, and model linear recovery

Inactive Publication Date: 2016-06-15
SICHUAN AGRI UNIV
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Problems solved by technology

The diagnosis of Parkinson's disease and its models mainly depends on behavioral evaluation, but the current behavioral scoring standards are uneven, with different emphases, and there is no complete set of clinical scoring standards specifically for primate PD models; in addition, currently The primate model cannot simulate resting tremors, Lewy bodies, and the model shows linear recovery and other shortcomings

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  • Method for establishing rhesus monkey model with chronic process Parkinson's disease
  • Method for establishing rhesus monkey model with chronic process Parkinson's disease
  • Method for establishing rhesus monkey model with chronic process Parkinson's disease

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Embodiment Construction

[0023] The specific implementation of the present invention will be described in conjunction with the examples.

[0024] 1. Materials and methods

[0025] 1.1 Selection of experimental animals: 10 healthy young rhesus macaques (subspecies of Western Sichuan macaque) were selected from the National Experimental Macaque Provenance Base (Ya'an, Sichuan) (animal use license: SCXK (Sichuan): 2013-105), with a body weight of 3.5- 5.5kg, aged 3.5-4.5 years old, see Table 1 for details. Adaptive single-cage feeding was carried out one month in advance, and free access to water and food. The handling of animals in the experiment complied with the regulations of the Ministry of Science and Technology of the People's Republic of China "Guiding Opinions on the Kindness of Experimental Animals".

[0026] 1.2. Inclusion criteria for test monkeys:

[0027] ①Age 4-4.5 years old

[0028] ②Weight 4-6kg;

[0029] ③ half male and half male;

[0030] ④ Have not received other experimental re...

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Abstract

The invention belongs to the technical field of life science and particularly relates to a method for establishing a rhesus monkey model with a chronic process Parkinson's disease (PD). By using the interventional mode that MPTP is injected for a long time starting from a smallest dose which is gradually increased according to the difference in animal sensitivity mainly by means of intramuscular injection assisted with intravenous injection, a rhesus monkey is slowly stimulated to generate all clinical symptoms of the PD. The success rate of the animal model established through the method for establishing the rhesus monkey model with the chronic process PD is 100%, lewy bodies are observed in the made animal model, and the method is used for evaluating the effect of new PD drugs and screening the new PD drugs.

Description

technical field [0001] The invention belongs to the technical field of life sciences, and in particular relates to a method for establishing a rhesus monkey model of chronic progressive Parkinson's disease. Background technique: [0002] Parkinson's disease (PD), also known as "parkinsonism", is the second largest degenerative disease of the central nervous system after Alzheimer's disease, and its main clinical manifestations are static, intentionality or Postural tremor, muscle rigidity, bradykinesia, and postural balance disorder, etc., and severe degeneration and loss of dopamine (DA) neurons in the substantia nigra compacta and Lewy bodies (lewybody, DA) appear in the remaining DA neurons in the brain LB) is the pathological feature. Tyrosine hydroxylase (TH) is the rate-limiting enzyme of dopamine synthesis, and its expression level can be used as an indicator of dopaminergic neuron activity. The incidence rate of people over 40 years old exceeds 0.1%. PD not only h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00
CPCA61D7/00
Inventor 罗启慧史良琴陈正礼曾文黄超龚立李立科刘衡刘文涛方静唐丽
Owner SICHUAN AGRI UNIV
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