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Mulberry parasitical phellinus strain wild environment returning rejuvenation culture method

A cultivation method, Phellinus technology, applied in the field of microbial cultivation, can solve the problems such as strain decline, and achieve the effect of solving degradation, stable and reliable strain source, and stable product quality

Inactive Publication Date: 2016-07-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for cultivating Phellinus parasitic Phellinus species in the wild environment for rejuvenation, which can effectively solve the problem of strain decline

Method used

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Embodiment Construction

[0012] A kind of mulberry tree parasitic Phellinus bacterium classification returns wild environment rejuvenation culture method, comprises the following steps successively:

[0013] A. Collection of wild Phellinus fruiting bodies: select fresh, complete, not yet lignified, and undamaged fruiting bodies, store at 4°C, and separate within 24 hours;

[0014] B. Isolation and purification of Phellinus strains: Rinse the surface of the fruiting body with sterile water to remove impurities in a sterile operating table, disinfect the surface of the fruiting body with 75% alcohol, cut out Phellinus 2 × 5mm tissue pieces with a scalpel and place them on a solid In plate medium, cultivate in a constant temperature incubator at 20-30°C. After Phellinus mycelium begins to grow, pick the vigorously growing mycelium at the top and further purify and cultivate it to obtain a purified Phellinus strain;

[0015] The Phellinus linteus (Inonotus linteus) used in the present invention comes fro...

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Abstract

The invention discloses a mulberry parasitical phellinus strain wild environment returning rejuvenation culture method. The method includes the steps that firstly, wild phellinus fruiting bodies are collected from mulberries, subjected to strain separation and purification and inoculated into a culture medium to be cultured, and purified phellinus strains are obtained; secondly, the phellinus strains are inoculated into artificial bag materials to culture phellinus fruiting bodies; thirdly, after artificial planting, purified phellinus strains are obtained by being separated from the artificially planted phellinus fruiting bodies; fourthly, the phellinus strains obtained from being separated from the artificial phellinus fruiting bodies are inoculated to wild mulberries, and phellinus fruiting bodies grow under natural conditions; fifthly, purified phellinus mother seeds are obtained through separation and purification of the phellinus fruiting bodies growing on the mulberries under the natural conditions, and artificial culture strain active rejuvenation is completed. The method has the advantages that strain activity can be naturally restored and improved, stable and reliable strain sources are provided for phellinus industrialized production, product quality is stabilized, and artificial cultivation of phellinus is continuously developed.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a method for rejuvenating and cultivating Phellinus spp. parasitic on mulberry trees in a wild environment. Background technique [0002] Phellinus linteus is a precious medicinal fungus that parasitizes broad-leaved trees such as mulberry. As early as 1968, Japanese scholar Tetsuro Ikekawa et al. have found that Phellinus has significant anti-tumor activity. Modern pharmacological studies have shown that Phellinus active substances inhibit the proliferation of tumor cells by arresting the cell cycle and inducing apoptosis. The research on Phellinus Phellinus in my country started relatively late. In recent years, due to the attention of many scholars and entrepreneurs, the enthusiasm for research and development has been unprecedentedly high. From October 17th to 19th, 2013, the "Ganoderma lucidum Phellinus Symposium on both sides of the Taiwan Strait" hosted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 钟石李有贵计东风陈伟国
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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