A kind of Lactobacillus plantarum cz401 strain producing exopolysaccharide and β-galactosidase and its application

A technology of Lactobacillus plantarum and galactosidase, applied in the direction of glycosylase, enzyme, bacteria, etc., can solve the problems of destruction, the survival rate is less than 0.1%, and the level of restoring the balance of human intestinal flora is limited.

Inactive Publication Date: 2019-05-17
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

At present, Streptococcus thermophilus and Lactobacillus bulgaricus, which make up the common yogurt starter, are mostly destroyed when they pass through the human digestive tract due to their intolerance to gastric acid and bile, and the survival rate is less than 0.1%, thus restoring the balance of the intestinal flora in the human body. level is very limited

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  • A kind of Lactobacillus plantarum cz401 strain producing exopolysaccharide and β-galactosidase and its application
  • A kind of Lactobacillus plantarum cz401 strain producing exopolysaccharide and β-galactosidase and its application
  • A kind of Lactobacillus plantarum cz401 strain producing exopolysaccharide and β-galactosidase and its application

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Embodiment Construction

[0010] The present invention will be described in detail below in conjunction with specific embodiments, but the protection scope of the present invention is not limited to the following embodiments.

[0011] Isolation and identification of strains

[0012] In this example, intestinal microbes were isolated from cheese made by herdsmen in the Inner Mongolia prairie and named CZ401. The morphological, physiological and biochemical characteristics of this strain are as follows: the colonies were light yellow when observed on MRS solid medium, indicating smoothness. It is round with a convex center; microscopic examination shows that the strain is Gram staining positive, rod-shaped, without flagella, and without spores.

[0013] The CZ401 strain was inoculated into 5ml MRS culture solution (casein peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; glucose 5.0g; sodium acetate 5.0g; citrate diamine 2.0g; Tween 80 1.0g; Dipotassium hydrogen phosphate 2.0g; Magnesium sulfate heptahydr...

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Abstract

The invention provides a Lactobacillus plantarum (L.plantarum) CZ401 strain producing exopolysaccharides and β-galactosidase, which was preserved in the China Type Culture Collection Center on March 7, 2016, and the address is: Wuhan University, Wuchang District, Wuhan City, Hubei Province, the preservation number is CCTCC NO: M2016092, and the 16S rDNA sequence of the strain is shown in SEQ ID NO.1. The bacterial colonies observed on MRS solid medium were light yellow, smooth, round, and raised in the center; microscopic observation showed that the strains were Gram-positive, rod-shaped, without flagella, and without spores. The above-mentioned CZ401 strain provided by the present invention not only has higher exopolysaccharide-producing ability and β-galactosidase activity, but also can ferment milk to produce lactic acid drinks, and has wide application value.

Description

Technical field [0001] The invention relates to a new strain of Lactobacillus, in particular to a Lactobacillus CZ401 that efficiently produces extracellular polysaccharide and β-galactosidase and its application. Background technique [0002] Lactic acid bacteria are recognized as a food-safe microorganism. At present, Streptococcus thermophilus and Lactobacillus bulgaricus, which make up ordinary yogurt starters, are mostly destroyed due to gastric acid and bile intolerance when they pass through the human digestive tract. The survival rate is less than 0.1%, thus restoring the balance of the human intestinal flora. The level is greatly restricted. Lactobacillus plantarum can effectively tolerate enzymes in the digestive tract, pH-valued gastric juice and bile acids. After entering the intestinal tract, it can adjust and improve the gastrointestinal tract and restore the balance of the intestinal flora. In addition, Lactobacillus plantarum has the functions of regulating bloo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C12N9/38A23C9/123C12R1/25
CPCA23C9/1234C12N9/2471C12P19/04C12Y302/01023C12N1/205C12R2001/25
Inventor 程国军马成杰殷杰田梦洋张欢欢何冬兰李晓华彭杨
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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