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White and mycorrhizal fungi and their application

A mycorrhizal fungus and mycelium technology, applied in the fields of application, fungi, and chemicals for biological control, etc., can solve problems such as difficult to meet cultivation needs, slow propagation speed of rhizome ramets, susceptibility to pathogenic bacteria, etc. To achieve the effect of protection and regeneration and sustainable utilization, the cultivation effect is remarkable, and the growth cycle is shortened

Active Publication Date: 2019-07-23
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional rhizome ramets have slow propagation speed, long growth cycle, low efficiency, high cost of seedlings, and it is difficult to meet the needs of large-scale cultivation; while sterile test-tube seedlings produced by tissue culture grow slowly, have low transplanting survival rate, and are resistant to stress. Poor sex, susceptible to pathogenic bacteria
Judging from the current situation, the difficulty in reproduction of white and white has not been fundamentally resolved, resulting in the near exhaustion of wild white and wild resources, and the market is in short supply, which seriously limits the demand for clinical medicine and industrial production of white and
[0006] In the prior art, there is no report on the application of fungi for promoting germination of Radix Radix Radix in the field. Therefore, screening and utilizing fungi that can promote the germination of Radix Radix Radix Radix Radix Radix Radix Radix L. is an effective way to realize the field cultivation of Orchidaceae. For It has important practical significance and application value to raise the seedlings and cultivation of Baiji, improve the quality and output of Baiji medicinal materials, and protect the resources of Baiji.

Method used

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  • White and mycorrhizal fungi and their application
  • White and mycorrhizal fungi and their application
  • White and mycorrhizal fungi and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Identification of strains

[0039] 1. Extraction of fungal DNA:

[0040] The fungi used for molecular identification were inoculated on PDA medium and cultured at 25°C in a dark room for 2 weeks. When the diameter of the fungal colony was 3 to 5 cm, the hyphae on the surface of the colony could extract DNA.

[0041] DNA extraction was carried out according to the instructions of the Fungal DNA Extraction Kit (E.Z.N.ATM Fungal DNA Kit, Omega Bio-Tek, Doraville, Georgia, USA). The specific steps are as follows:

[0042] 1) Scrape 50-100mg of mycelium into a sterilized mortar, add liquid nitrogen to freeze, immediately grind into powder, and scrape the ground hyphae into a sterilized 1.5mL Eppendorf tube.

[0043] 2) Add 800μL of Buffer FG1 buffer to the Eppendorf tube, vortex and mix well, then in a water bath at 65°C for 10 minutes, and invert and mix twice during the water bath.

[0044] 3). Then add 140μL Buffer FG2, vortex and mix well, and centrifuge at 13300r / min for 10min at ...

Embodiment 2

[0080] 1. First use PDA solid medium to activate the white and mycorrhizal fungus strains at a culture temperature of 26±2℃, cultivate in the dark for 7-15 days, then pick several small pieces from the edge of the formed colony and inoculate them into the liquid medium Carry out dark culture on a shaker, the rotating speed of the shaker is 100r / min~150r / min, the culture temperature is 26℃±2℃, and the culture is dark for 10-20 days. After the culture, the obtained mycorrhizal fungus mycelium can be used directly. It can also be used after crushing with a soymilk machine.

[0081] 2. The effect of liquid medium on mycorrhizal fungus mycelium

[0082] The liquid culture medium is composed of glucose, inorganic salts and natural extracts. The amount of glucose added is 15g / L-40g / L, and the inorganic salt is MgSO 4 ·7H 2 O and KH 2 PO 4 The natural extract is one of potato extract, wheat bran extract, and oat extract.

[0083] 1) For MgSO in liquid medium 4 ·7H 2 Experiment with the amou...

Embodiment 3

[0107] The inventor took 200 white and white seeds and divided them into two groups. In 1 group, white and white seeds and white and mycorrhizal fungal mycelium were used as materials according to the method in Example 2 to produce white and white seeds and white and mycorrhiza. After the symbiotic mixture of fungi, they were directly sown in the field. In the second group, the seeds were sown directly in the field. At the 5th week after sowing, the first group began to germinate. The seed germination in 5-12 weeks was measured as shown in Table 5. Show:

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Abstract

The invention discloses a white and mycorrhizal fungus. The white and mycorrhizal fungus is Sebacinales sp. mycorrhizal fungus strain YY‑51. The white and mycorrhizal fungus was preserved in China on July 31, 2015. The General Microbiology Center of the Microbial Culture Collection Management Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is GCMCC NO.11104. In the present invention, the mycelium of the mycorrhizal fungus of the bletilla and the seed of the bletilla can promote the germination of the seed of the bletilla and the germination rate can reach 98%.

Description

Technical field [0001] The invention relates to the field of biotechnology. More specifically, the present invention relates to a white and mycorrhizal fungus and its application. Background technique [0002] Orchidaceae is a larger family of angiosperms. It contains many rare and valuable medicinal plants and flowers, such as Dendrobium officinale, Nocturna, Baiji, etc. It is also a special ecology with a high degree of evolution in the plant kingdom and closely related to fungi. Taxa. Mycorrhizal fungi are indispensable for the seed germination, seedling growth, or plant formation of orchids. Orchid mycorrhizas are particularly important in the protection, introduction, domestication and artificial cultivation of orchids for medicinal and ornamental plants. However, since most orchids are endangered, all wild species of orchids have been included in the "Convention on International Trade in Endangered Wild Fauna and Flora", which is the "flagship" group in plant protection. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00A01C1/02C12R1/645
CPCA01C1/02A01N63/30C12N1/145C12R2001/645
Inventor 谭小明周雅琴缪剑华余丽莹
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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