Preparation method of hand-foot-and-mouth disease etiology colloidal gold diagnosis detection test paper box

Abstract

The invention relates to a preparation method of a hand-foot-and-mouth disease etiology colloidal gold diagnosis detection test paper box, and the method is capable of effectively solving the problems of low detection sensitiveness, low accuracy, high detection leakage possibility and difficult specimen collection in the prior art. The method comprises the following steps of: firstly adhering a reaction film to the center of a backlining; spraying a detection line and a control line on the reaction film; pressing a water absorption cushion to one side, which is adjacent to the tail end of the backlining, of the reaction film, pressing a colloidal gold cushion to the other side of the reaction film, pressing a sample cushion to the other side of the colloidal gold cushion; fixing the sample cushion, the colloidal gold cushion, the reaction film and the water absorption cushion to the backlining; cutting into 4mm-width strips along the cross section direction of the backlining by use of a slitter; assembling testpaper into a testpaper shell, and forming a sample hole and a detection result region on the test paper shell. The high-sensitivity hand-foot-and-mouth disease etiology rapid detection test paper prepared by anti-enterovirus 71 type specific monoclonal antibodies and anti-coxsackievirus A16 type specific monoclonal antibodies is good in detection sensitivity, high in accuracy and low in detection leakage possibility.

Description

technical field [0001] The invention relates to a detection test paper, in particular to a preparation method of a colloidal gold diagnostic detection test paper box for hand, foot and mouth disease pathogens. Background technique [0002] Hand foot and mouth disease (Handfoot and mouth disease, HFMD) is a common infectious disease caused by human enterovirus. It mostly occurs in children under 5 years old, and can cause herpes on hands, feet, mouth and other parts. A small number of children can cause complications such as myocarditis, pulmonary edema, and aseptic meningoencephalitis. If the condition of individual critically ill children develops rapidly and leads to death, it poses a huge threat to the life and health of infants and young children, causing serious social impact. There are more than 20 types of enteroviruses that cause HFMD, among which Coxsackievirus A16 (CA16) and enterovirus 71 (EV71) are the most common. According to statistics from the Ministry of H...

AI Technical Summary

Problems solved by technology

[0007] For above-mentioned situation, in order to solve the defect of prior art, the purpose of the present invention is exactly to provide a kind of preparation method of colloidal gold diagnostic test paper box of hand, foot and mouth disease pathogen, can effectively solve prior art detection sensitivity is low, and accuracy rate is low, It is easy to miss the detection and the sample collection is difficult.

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