Method for screening biomarkers of ionizing radiation as well as application of SZT2 protein determined by method

Abstract

The invention relates to a method for screening biomarkers of ionizing radiation as well as an application of SZT2 protein determined by the method to preparation of a molecular biomarker kit for personnel exposed to heavy ion radiation. The method comprises the following steps: screening differentially expressed genes in human cells which are treated with heavy ion irradiation by using a genechip method; screening differentially expressed proteins in cells of the same type which are treated with heavy ion irradiation by using iTRAQ technology; comparing screening results, and from the genes and the proteins which are differentially expressed simultaneously, selecting differentially expressed protein SZT2 which is down-regulated both in gene and protein levels as the biomarker of ionizing radiation. The genechip method and iTRAQ technology are combined, categories and quantities of genes and proteins in cell specimens of experimental groups of different dosages and a control group are compared and analyzed, the differentially expressed protein SZT2 which is down-regulated both in gene and protein levels can be rapidly found, and the protein can be used as a significant biomarker of ionizing radiation.

Description

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AI Technical Summary

Benefits of technology

This patented technique uses an electrospray device that generates charged particles called plasma (a type of gas) which helps trigger specific changes on DNA within cancerous tissues caused by exposure therapy. By combining this process with analysis from experiments conducted under various conditions, it becomes possible to identify differences between treated individuals who have been subjected to high level radioactive rays compared against healthy ones. These techniques help predict how well these treatments will work over time.

Problems solved by technology

This patents describes various ways to measure biomedical indicators related to ionization radiation. These measures involve analyzing how cells react when exposed to high dosage rates of ionized radiation. They also provide an accurate way to determine if there were any harm during radiotherapy due to their effects. Biomic radiomic signatures refer specifically to chemicals released into our bodies through interactions with electrical fields and nuclear receptors. By comparing them against known sources, we may make inferences about possible injury mechanisms associated with ionizing rays.

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