An electrochemical measurement method

A measurement method and electrochemical technology, applied in the field of electrochemical detection, can solve the problems affecting the measurement accuracy and stability of electrochemical test strips

Active Publication Date: 2018-07-06
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the above three methods can reduce or eliminate the interference signal to a certain extent, the measurement accuracy and stability of the electrochemical test strip will be affected when the interference signal is deducted.

Method used

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Embodiment 1

[0070] use figure 1 The electrochemical test strip shown has glucose oxidase, electron mediator (potassium ferricyanide), binder (hydroxymethyl cellulose) and stabilizer (sucrose) on the surface of the working electrode, and the surface of the blank electrode and auxiliary electrode contains Electron mediator (potassium ferricyanide), binder (hydroxymethyl cellulose) and stabilizer (sucrose), the electrochemical test strip is inserted into the constant potential electrochemical measurement current meter of the model CHI760, and the working electrode The same positive potential (+0.3V) relative to the auxiliary electrode is applied to the blank electrode at the same time, then the total current signal S measured by the working electrode w It is related to the concentration of glucose and interfering substances, while the interference current signal S obtained by blank electrode measurement B related to the concentration of interferents. By using electrochemical test strips to...

Embodiment 2

[0081] This embodiment is an application example of a glucose electrochemical measuring instrument that eliminates the negative interference caused by the interference by applying the negative potential and using the reduction current as the detection signal.

[0082] use figure 2 Electrochemical test strip shown with glucose oxidase, ascorbate oxidase, peroxidase, electron mediator (potassium ferrocyanide), binder (hydroxymethylcellulose) and stabilizer on the surface of the working electrode (sucrose); the surface of blank electrode and auxiliary electrode contains electron mediator (potassium ferrocyanide), ascorbate oxidase, peroxidase, binder (hydroxymethyl cellulose) and stabilizer (sucrose). The chemical test strip is inserted into the constant potential electrochemical measurement ammeter of model CHI760, and the same negative potential (-0.2V) relative to the auxiliary electrode is applied to the working electrode and the blank electrode at the same time, then the cu...

Embodiment 3

[0094] This embodiment is an application example of a glucose electrochemical measuring instrument that uses the oxidation current as the detection signal to eliminate the positive interference signal caused by the high temperature environment when a positive potential is applied.

[0095] use figure 1 As shown in the structure of the test strip in 1-3, the surface of the working electrode contains glucose oxidase, electron mediator (potassium ferricyanide), binder (hydroxymethyl cellulose) and stabilizer (sucrose), blank electrode and auxiliary electrode The surface contains electron mediator (potassium ferricyanide), binder (hydroxymethyl cellulose) and stabilizer (sucrose), and the prepared blood glucose electrochemical test strip is placed in a 60°C incubator for 15 days (accelerated aging ) after rewarming for 1 hour, and then insert the electrochemical test strips at room temperature and after accelerated aging into the constant potential electrochemical measuring curren...

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Abstract

The invention provides an electrochemical measurement method. The method comprises the following steps: measuring a sample by adopting an electrochemical test bar comprising a working electrode, a blank electrode and an auxiliary electrode so as to obtain a working electrode current signal Sw and a blank electrode current signal SB; correcting the Sw according to a correlation between the SB and a background signal base value SB0 prestored in a device so as to obtain a corrected working electrode current signal S(corrected), wherein if the SB is not greater than the SB0, the corrected working electrode current signal S(corrected) is equal to Sw, if the SB is greater than the SB0, the corrected working electrode current signal S(corrected)= Sw+ / -(SB-f*SB0), and f is a coefficient of correction prestored in the device and is greater than 0. According to the method, the Sw and the SB are obtained through testing, and the Sw is corrected by using a logical decision relationship between the SB and the background signal base value SB0 and algorithm processing so as to obtain the S(corrected); interfering signals can be effectively deducted, and the accuracy and stability of the electrochemical test bar are improved.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to an electrochemical measurement method for improving the accuracy and stability of an electrochemical test strip. Background technique [0002] In the field of medical in vitro diagnostics (IVD), especially in the field of timely detection (POCT), the electrochemical measurement system based on the electrochemical method of amperometric bioenzyme sensor has an important application in the detection of biomolecules in body fluids (blood or urine). The basic principle is to make a measuring electrode with a bioenzyme sensor. The target analyte and the enzyme layer substance in the measuring electrode undergo an enzymatic redox reaction, and apply a certain positive or negative potential to the measuring electrode through an electrochemical measuring instrument, and measure The electrochemical sensor responds to the oxidation or reduction current signal of the poten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 吕国文
Owner SINOCARE
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