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A method of artificial reproduction of beluga and the application of carp pituitary gland

A technology for artificial breeding and male fish of A. hulus, which is applied to the pituitary gland of carp and the field of artificial breeding of A. hulus. It can solve the problems of germplasm degradation of A. hulus artificial breeding, and achieve good fertilization and hatching effects, a high rate of induced labor, and a good survival condition. Effect

Inactive Publication Date: 2017-10-24
BEIJING FISHERMAN AQUATIC PROD TECH DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of the prior art such as the severe degradation of the germplasm of the artificial breeding of the Chinese beluga, and to provide a method for rapid artificial breeding of the beluga and the application of the carp pituitary gland

Method used

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  • A method of artificial reproduction of beluga and the application of carp pituitary gland
  • A method of artificial reproduction of beluga and the application of carp pituitary gland

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0053] This preparation example is used to illustrate the preparation of the pituitary gland solution of the carp pituitary, which is used for inducing labor of the carp of the invention.

[0054] Carp pituitary gland dry matter 200mg, weighed and then crushed and dissolved in 10ml 15wt% glucose solution, of which, 10ml 15wt% glucose solution used 2.2ml 50wt% glucose solution and 7.8ml 5wt% glucose solution Prepared from glucose solution.

preparation example 2

[0056] This preparation example is used to illustrate the preparation of the pituitary gland solution of the carp pituitary, which is used for inducing labor of the carp of the present invention.

[0057] The dry matter of carp pituitary gland was 228 mg, weighed and then crushed and dissolved in 12 ml of 10% by weight glucose solution. 10ml 15wt% glucose solution needs to be prepared with 1.3ml 50wt% glucose solution and 13.7ml 5wt% glucose solution.

Embodiment 1

[0059] This embodiment is used to illustrate the method of artificial reproduction of the shoki and the application of the oxytocin drugs in the artificial reproduction of the shoki.

[0060] (1) At 8 months before the need to induce spawning, choose the female parent of the European catfish (bred for 13 years, and its egg diameter has reached 3.3mm, weight 50kg) and the parent of the male catfish (bred for 11 years, white piercing naked eyes Semen, weighing 50kg), and then nutrient-enhanced cultivation of the female and male parents of the European catfish for 150 days. The water quality control conditions are: the cultivation pond is 25m×8m×1.5m cement pond, the water depth is 1m, and the water flow rate is 0.1m per second. 3 , The water temperature is 20℃, so that the dissolved oxygen is 6mg / L, the ammonia nitrogen content is 0.4mg / L, and the nitrite is 0.05mg / L; the control feeding method is: feed once a day, the feeding rate is 0.3%, The protein content in the compound feed i...

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Abstract

The invention relates to the field of beluga culture, particularly discloses a beluga artificial propagation method and application of a carp pituitary gland, and relates to a beluga artificial propagation method. The method comprises the steps that 1, nutrient enrichment culture is performed on a beluga milter parent and a beluga raun parent; 2, the beluga milter parent and the beluga raun parent obtained after nutrient enrichment culture are subjected to low temperature stimulation; 3, the water temperature is increased to the pre-spawning induction temperature, and spawning induction medicine administration is performed on the beluga milter parent and the beluga raun parent so as to be subjected to spawning induction; 4, free eggs and seminal fluid are collected to be subjected to artificial fertilization and incubation, wherein the spawning induction medicine is the carp pituitary gland. The invention further relates to application of the carp pituitary gland for beluga artificial propagation, and application of the carp pituitary gland for preparing medicine for artificial propagation of beluga. The beluga spawning induction medicine is easy to obtain and prepare, the spawning induction rate of spawning induction propagation performed by adopting the beluga spawning induction medicine is high, the fertilization incubation effect is good, and the postpartum parent fish survival condition is good.

Description

Technical field [0001] The present invention relates to the field of brevis breeding, in particular to a method for artificial reproduction of brevis and the application of carp pituitary. Background technique [0002] Because Huso huso is a large fish, it is a fierce carnivorous fish with the advantages of good meat quality and fast growth. It is one of the largest existing freshwater fishes, weighing more than 1 ton. Its sexual maturity takes a long time and requires more than 12 years under artificial breeding conditions. The caviar made from its eggs is the best caviar, and the price is several or ten times higher than that of other sturgeons. Introduced for the first time in China in 2002, its induced spawning and reproduction are different from ordinary sturgeons. Only in 2013, Ren Hua et al. (article number: 1006-3188(2013)-01-0020-03) research on the artificial reproduction technology of European sturgeon In this document, the method of artificial injection of dioxone (D...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A61K35/60A61P15/08
CPCA01K61/00A61K35/60Y02A40/81
Inventor 齐茜刘晓勇依布拉英·买丽坎白喜军
Owner BEIJING FISHERMAN AQUATIC PROD TECH DEV CENT
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