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Cholesterol metabolism detection markers and their application

A technology for cholesterol and total cholesterol, applied in the field of cholesterol metabolism detection markers, can solve the problems of elevated, elevated, and increased risk of cardiovascular disease in plant sterols, achieving significant clinical significance and improving therapeutic effects.

Active Publication Date: 2018-10-02
广东国盛医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The intestinal absorption of cholesterol is significantly increased, which may increase the risk of cardiovascular disease. Existing studies have found that ABCG5 and ABCG8 gene mutations, significantly increased plant sterols, and premature heart disease

Method used

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  • Cholesterol metabolism detection markers and their application
  • Cholesterol metabolism detection markers and their application
  • Cholesterol metabolism detection markers and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 7-encholesterol and streptosterol as markers for detecting cholesterol synthesis status

[0033] In order to study whether 7-encholesterol and streptosterol can be used as markers for detecting cholesterol synthesis, this example conducted a study on 61 random middle-aged males, and found that 7-enecholesterol and streptosterol were closely related to cholesterol in plasma. The concentration changes were negatively correlated (as shown in Table 1). The plasma cholestanol concentration is an important indicator of high intestinal cholesterol absorption efficiency and low plasma cholesterol synthesis. This proves that 7-encholesterol and streptosterol may be used as markers of cholesterol synthesis and absorption.

[0034] Table 1 Correlation between 2 kinds of non-cholesterol steroids and plasma cholestanol concentration (n=61)

[0035]

[0036] *P<0.01; **P<0.05

[0037] In addition, when observing the synthesis and absorption of cholesterol in 61 cases ...

Embodiment 2

[0044] In order to further study the role of 7-encholesterol and streptosterol in detecting cholesterol synthesis, the present invention finally obtains a formula that can well evaluate the cholesterol synthesis level in the human body through the research and verification of a large number of experiments in the early stage:

[0045]

[0046] Among them, 7-ene cholesterol (Lathosterol) ratio = C lathosterol / TC (ratio unit: μmol×100 / mmol), that is, the ratio of 7-ene cholesterol is the ratio of 7-ene cholesterol concentration (μM) / total cholesterol concentration (mM) and multiplied by 100.

[0047] Desmosterol ratio = C desmosterol / TC (ratio unit: μmol×100 / mmol); that is, the ratio of streptosterol is the ratio of streptosterol concentration (μM) / total cholesterol concentration (mM) multiplied by 100.

[0048] Campesterol ratio = C campesterol / TC (ratio unit: μmol×100 / mmol); that is, the ratio of campesterol is the ratio of campesterol concentration (μM) / total cholester...

Embodiment 3

[0052] Example 3 Quantitative detection of 7-ene cholesterol, streptosterol, campesterol and β-sitosterol

[0053] 1) Preparation of reference solution

[0054] Accurately weigh the reference substances of 7-cholesterol, streptosterol, campesterol, and β-sitosterol respectively, and use n-hexane as the solvent to prepare 11.00, 6.70, 18.00, and 33.00 mg·L -1 solution for use as a stock solution. Take the stock solution and dilute it with n-hexane to prepare a mixed control solution with the highest mass concentration of No. 7, each containing 4 substances to be tested, and then successively dilute it step by step. See Table 3 for specific mass concentrations.

[0055] Table 3 Concentrations of 7-cholesterol, strepsterol, campesterol, and β-sitosterol in each group of reference substances after dilution

[0056]

[0057] 2) Preparation of internal standard solution

[0058] Accurately weigh 0.0125g of 5α-cholestane in a 25mL measuring bottle, add n-hexane to dilute to the ...

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Abstract

The invention discloses detection markers of cholesterol metabolism and applications thereof. Researches show that lathosterol and desmosterol can be used as markers for detecting cholesterol anabolism state; if cholesterol is excessively synthesized in human body, high risk of cardiovascular and cerebrovascular diseases and high morbidity rate of early onset cardiovascular disease are promoted, and in the aspect of pharmacy, statins are suggested for treatment. Lathosterol and desmosterol can be used for accurately detecting dynamic conditions of cholesterol synthesis in vivo; the detection result can be used as a basis for doctors to provide accurate treatment for patients, pharmacy is guided, treatment effects are improved, and the clinic meanings are substantial.

Description

technical field [0001] The invention relates to a cholesterol metabolism detection marker and its application. Background technique [0002] A large number of epidemiological and clinical studies support that elevated low-density lipoprotein cholesterol (LDL-C) levels are one of the important risk factors for the development of atherosclerosis. International and domestic guidelines for the prevention and treatment of dyslipidemia unanimously recommend that reaching the standard LDL-C level is the primary goal for the prevention and treatment of coronary heart disease. However, the current situation of blood lipid control in our country is not optimistic. The second multi-center collaborative study on clinical blood lipid control in China showed that the overall compliance rate of dyslipidemia control in my country was only 38%, and the compliance rates of low-risk, medium-risk, high-risk and extremely high-risk patients were 91%, 77%, and 49%, respectively. %, 38%. The uns...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/025
Inventor 邓杏飞潘树球
Owner 广东国盛医学科技有限公司
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