Unlock instant, AI-driven research and patent intelligence for your innovation.
Beta-human chorionic gonadotropin determination kit and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A chorionic gonadotropin and kit technology is applied in membrane chromatography technology, labeling immunoassay technology, β-human chorionic gonadotropin assay kit, protein cross-linking technology field, and can solve the problem of high intensity and strong interference, etc. problems, to achieve the effect of rapid response, high sensitivity, and clear clinical significance
Inactive Publication Date: 2017-04-26
WEIHAI NEOPROBIO
View PDF8 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
In the usual fluorescence measurement, because the test sample contains a variety of fluorescent components, the background fluorescence (scattered light caused by colloidal particles and solvent molecules in the
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0044] The components of the test paper card in the β-human chorionic gonadotropin assay kit can be prepared by the following measures:
[0045] 1. Preparation of sample pad 2:
[0046] Soak the glass fiber membrane in the treatment solution containing 1.0% Triton X-100, 2.5% BSA, 0.15M Tris buffer, pH7.5, soak at 4°C for 4 hours, then place it in an oven and dry it at 37°C 2 hours.
[0047] 2. Preparation of binding pad 3 for absorbing fluorescent microsphere-labeled antibody:
[0048] Soak the glass fiber membrane in 150mM Tris-HCL treatment solution (containing 1.0% Triton X-100, 2.5% BSA, pH7.4), soak for 2 hours at 4°C, then take it out of the oven at 37°C and dry it for 4 hours, and set aside. The glass fiber membrane was placed on the Bio-DotXYZ3050 three-dimensional spraying platform, and the rare earth Eu 3+ The conjugated complex of anti-β-human chorionic gonadotropin monoclonal antibody labeled with fluorescent microspheres is sprayed onto the glass fiber membran...
Embodiment 2
[0057] Embodiment 2: accuracy test
[0058] Select the above test paper card and fluorescence immunochromatography analyzer (model: NEO-007),
[0059] Setting of the parameters of the fluorescence immunoassay analyzer: After setting the process parameters of the test paper card on the fluorescence immunoassay analyzer, take the above assembled test paper card and use 5, 500, 1000, 5000, 10000, 20000, 40000mIU / mL respectively The β-human chorionic gonadotropin calibrator is measured with a test paper card to obtain the fluorescence intensity value of each calibrator, and the result is input into the parameters of the analyzer to complete the parameter setting of the analyzer.
[0060] Main testing materials: clinical samples obtained from relevant hospitals, a total of 300 latex-enhanced immunoturbidimetric value samples, including 100 serum samples, 100 plasma samples, 100 whole blood samples, content distribution of β-human chorionic gonadotropin The interval is between 5-40...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
Diameter
aaaaa
aaaaa
Diameter
aaaaa
aaaaa
Login to View More
Abstract
The invention relates to the technical field of fluorescence immunochromatography in medical immunology, and in particular, relates to a beta-human chorionic gonadotropin determination kit and a preparation method thereof. The kit is provided with a test paper card and is characterized in that the test paper card is successively provided with a PVC plate, a sample pad, a combination pad, a cellulose nitrate membrane and a water absorbent pad from bottom to top, wherein the combination pad is adsorbed with rare earth Eu<3+> fluorescent microsphere-labeled beta-human chorionic gonadotropin monoclonal antibody, and rare earth fluorescent microspheres have the diameter of 150 nm, contain a rare earth lanthanide element Eu<3+>, are stable under a ground state and emit fluorescence with the wavelength of 615 nm under the action of a 337 nm excitation light source; the monoclonal antibody is a monoclonal antibody obtained by mixing after purification and is derived from monoclonal antibody cell lines for 2-6 different beta-human chorionic gonadotropin antigen epitopes; the kit has the advantages of simple operation, quick response, high sensitivity, high specificity and the like.
Description
[0001] Technical field: [0002] The invention relates to the technical field of fluorescence immunochromatography in medical immunology, including protein cross-linking technology, membrane chromatography technology, marker immunoassay technology and the like. Specifically, it is a beta-human chorionic gonadotropin assay kit and a production method that can quickly and accurately perform quantitative analysis of beta-human chorionic gonadotropin in samples such as serum, plasma, and whole blood. [0003] Background technique: [0004] Human chorionic gonadotropin (Human Chorionic Gonadotropin, HCG) is a kind of glycoprotein hormone secreted by placental trophoblast cells. It consists of two subunits, α and β, with a sugar content of about 30%. The α-subunit is composed of 92 amino acid residues and has a molecular weight of 14.5KD. It is similar in structure to the α-subunits of FSH, LH and TSH. The β-subunit is a specific chain composed of 145 amino acids and has a molecular ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More