Influenza A (H1N1) virus IgM and total antibody rapid detection test paper

A technology for detecting influenza virus and test strips, which is applied to measuring devices, instruments, scientific instruments, etc., to achieve the effects of strong specificity, high sensitivity, and easy operation

Inactive Publication Date: 2010-07-28
天津中新科炬生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of antibody detection, there are detection methods such as ELISA, but there is no relevant report on the detection of type A H1N1 IgM antibody using colloidal gold-based immunochromatography technology

Method used

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  • Influenza A (H1N1) virus IgM and total antibody rapid detection test paper
  • Influenza A (H1N1) virus IgM and total antibody rapid detection test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Embodiment: Preparation of type A H1N1 influenza virus IgM and total antibody rapid detection test paper

[0020] 1 main material

[0021] 1.1 Specific H1N1 influenza virus Ha antigen: Sino Biological Inc. product, recombinant antigen, used for colloidal gold labeling; mouse anti-human IgM: product of Academy of Military Medical Sciences, anti-Ha antibody, self-made with Ha immunized goats, used in NC Membrane coating; chloroauric acid: product of Sigma Company; nitrocellulose (NC) membrane: product of Millipore Company; bovine serum albumin (BSA), polyethylene glycol PEG20000, hydrolyzed casein: product of Sigma Company. Other commonly used reagents are analytical reagents.

[0022] 1.2 The clinical serum was obtained by the company in relevant hospitals, among which 80 samples were recently vaccinated with H1N1 vaccine and 120 samples were not vaccinated.

[0023] 1.3 Influenza A H1N1 IgM and IgG ELISA Kit, Sino Biological Inc. product.

[0024] 2 methods

[0025]...

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PUM

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Abstract

The invention relates to the technical field of biological application, in particular to influenza A (H1N1) virus IgM and total antibody rapid detection test paper. The influenza A (H1N1) virus antigen is marked by adopting colloidal gold; and the detection of the influenza A (H1N1) virus IgM in blood is realized by coating anti-human IgM and the influenza A (H1N1) virus antigen. The virus IgM and the test paper have the advantages of simple and convenient operation, quick response, high sensitivity, high specificity, suitability for field test, economy and practicality, and the like.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to a rapid detection test paper for rapid detection of IgM and total antibody of influenza A (H1N1) virus by means of colloidal gold immunochromatography. Background technique [0002] Influenza A (H1N1) virus is a new type of influenza virus, which became a worldwide pandemic in 2009 and seriously endangered human health. Its "Ha" refers to hemagglutinin (Hemagglutinin), and "Na" refers to neuraminidase (Neuraminidase). Influenza A virus H has types 1-15, and types 1-9. H1N1 is a type of influenza virus and one of the most harmful viruses. It has caused several pandemics in history. [0003] At present, the main methods for detecting Influenza A (H1N1) are PCR gene amplification diagnostic method, nasopharyngeal test strip antigen diagnostic method and so on. In terms of antibody detection, there are detection methods such as ELISA, but there is no relevant repor...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/531G01N33/532
Inventor李洲杨发青
Owner天津中新科炬生物制药股份有限公司