Immunofluorescence quantitative test strip for detecting N-terminal atrial natriuretic peptide

A technology of atrial natriuretic peptide and immunofluorescence, applied in the field of immunology detection, can solve the problems of low automation, poor quantitative accuracy, radioactive contamination, etc., and achieve the effect of simple operation, high accuracy and rapid response

Inactive Publication Date: 2018-04-20
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the ELISA method has poor quantitative accuracy, long operation time, and low degree of automation, and is mostly used for qualitative detection; the radioimmunoassay method can reach a sensitivity of 4pg / ml, and is simple to operate and accurate in measurement. Radiation risk; electrochemiluminescence method has strong specificity, high sensitivity and high accuracy, but requires expensive equipment and experienced operators, and is generally used in specific medical institutions

Method used

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  • Immunofluorescence quantitative test strip for detecting N-terminal atrial natriuretic peptide
  • Immunofluorescence quantitative test strip for detecting N-terminal atrial natriuretic peptide
  • Immunofluorescence quantitative test strip for detecting N-terminal atrial natriuretic peptide

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Experimental program
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Embodiment 1

[0022] Embodiment 1 Storage solution formula and preparation method

[0023] The preferred formulation of the storage solution of the present invention: the mass concentration of PB is 20mM, the percentage concentration of BSA is 1.8%, the percentage concentration of Tween-80 is 0.5%, the percentage concentration of glucose is 0.5%, and the percentage concentration of glycine is 2%, the percentage concentration of PEG4000 is 1%, the percentage concentration of PEG20000 is 1.5%, the percentage concentration of Proclin300 is 0.03%, pH7.8~8.0.

[0024] Preparation method of storage solution: Weigh 0.25g of glucose and dissolve it in 45mL of pure water. Add 1mL of 100mM PB stock solution prepared in advance, shake and mix well. Add 1g of glycine, 0.5g of PEG4000, 0.75g of PEG20000 and 0.9g of BSA successively, shake and mix well; add 0.25mL Tween-80 with a sample gun, mix well repeatedly; add 15μL of Proclin300, shake and mix well, adjust the pH value to 7.8 with 1M HCl ~8.0. F...

Embodiment 2

[0026] Example 2 Combination Pad Pretreatment Liquid Formula and Preparation Method

[0027] The preferred formula of the binding pad pretreatment liquid of the present invention: the percentage concentration of PVA (polyvinyl alcohol) is 2.0%, the percentage concentration of TritonX-100 is 0.8%, the percentage concentration of sucrose is 1.25%, Proclin300 or sodium azide The percentage concentration is 0.03%, PH7.2~7.4.

[0028] Preparation method of bonding pad pretreatment solution: Weigh 10g of PVA and add 480ml of pure water to soak overnight, place at 60°C and heat to dissolve. Add 4mL of Triton X-100 with a sample gun, beat repeatedly; weigh 6.25g of sucrose, put it into a magnetic stirrer to dissolve; add 150μL of Proclin300, adjust the pH value to 7.2-7.4 with 1M HCl, shake and mix. Finally, the volume was adjusted to 500 mL at room temperature, and the filter was sterilized to prepare the binding pad pretreatment solution.

Embodiment 3

[0029] Example 3 N-terminal atrial natriuretic peptide immunofluorescence quantitative test strip

[0030] The preparation process of the N-terminal atrial natriuretic peptide immunofluorescence quantitative test strip is as follows:

[0031](1) Preparation of storage solution: the mass concentration of PB is 20mM, the percentage concentration of BSA is 1.8%, the percentage concentration of Tween-80 is 0.5%, the percentage concentration of glucose is 0.5%, and the percentage concentration of glycine is 2%, the percentage concentration of PEG4000 is 1%, the percentage concentration of PEG20000 is 1.5%, the percentage concentration of Proclin300 is 0.03%, pH7.8~8.0, prepare and mix according to the above formula, filter and sterilize to prepare the storage solution ;

[0032] (2) Preparation of the sample pad: soak the glass fiber membrane with the sample pad treatment solution for 10 minutes, place it in a drying room at 37°C and 30% humidity, and dry it for 5 hours to prepare...

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Abstract

The invention discloses an immunofluorescence quantitative test strip for detecting N-terminal atrial natriuretic peptide. The immunofluorescence quantitative test strip for the N-terminal atrial natriuretic peptide, which is prepared by matching a stock solution with a conjugate pad pretreating solution in the invention, has the characteristic that the precision is good; after the immunofluorescence quantitative test strip is accelerated at the temperature of 37 DEG C for 7 days, a detection result shows that the precision is reduced within 5 percent; the deviation between a detection value and a theoretical value is small and the detection accuracy is high; in the detection result, a peak outlet effect is better, a peak outlet signal is high, a base line is smooth, a front part hardly raises up, the influence of software on calculation of peak area is avoided, and reading accuracy is high; fluorescent microspheres have a better release effect and calculating area of the software is more accurate and related. The immunofluorescence quantitative test strip is suitable for clinical detection and has the advantages of good clinical guidance significance, simplicity and convenience inoperation, quick reaction, high sensitivity, high specificity, suitability for field detection, economy, practicality and the like.

Description

technical field [0001] The invention relates to the field of immunological detection, and more specifically relates to an immunofluorescence quantitative test strip for detecting N-terminal atrial natriuretic peptide. Background technique [0002] Heart failure is a group of syndromes caused by various structural or functional diseases of the heart that lead to impaired ventricular filling and / or ejection ability, and it is one of the most common causes of hospitalization or death in the elderly. With the aging of the population and the increase in the survival rate of myocardial infarction, heart failure is the only cardiovascular disease whose incidence and prevalence are still rising, and its prevention and treatment has become a hot spot in clinical cardiology research in recent years. Studies in recent years have shown that N-terminal pro-B-type natriuretic peptide (NT-proBNP) has a higher value in early diagnosis, and is the best myocardial marker for diagnosing heart ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/533G01N33/543G01N33/577G01N33/558
CPCG01N33/6887G01N33/533G01N33/54313G01N33/558G01N33/577G01N2333/4712G01N2800/325
Inventor 陈润文何平李冰肖丝尹周琼华
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG
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