Method for constructing sweet potato core germplasm resource library based on SSR (simple sequence repeat) molecule markers

A technology of core germplasm and molecular markers, applied in the field of plant germplasm resource evaluation, can solve problems such as the construction method of sweet potato core germplasm that has not yet been seen, and achieve the effect of clear and stable banding, good stability, and strong representativeness

Pending Publication Date: 2017-05-10
MAIZE RES INST GUANGXI ACADEMY OF AGRI SCI
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Problems solved by technology

[0009] Up to now, there is no relevant report on the construction m

Method used

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  • Method for constructing sweet potato core germplasm resource library based on SSR (simple sequence repeat) molecule markers
  • Method for constructing sweet potato core germplasm resource library based on SSR (simple sequence repeat) molecule markers
  • Method for constructing sweet potato core germplasm resource library based on SSR (simple sequence repeat) molecule markers

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Embodiment 1

[0035] 1. Materials and methods

[0036] 1.1 Materials

[0037] The 378 accessions of sweet potato germplasm resources were from sweet potato planting areas at home and abroad, and were divided into 4 groups, of which Group I came from southern potato areas in China (297 accessions), Group II came from domestic potato areas in the middle and lower reaches of the Yangtze River (51 accessions), and Group III Group 4 came from the northern potato region in China (23 samples), and Group Ⅳ came from foreign countries (7 samples). The above materials were all planted in the sweet potato germplasm resource nursery of Mingyang Base, Guangxi Academy of Agricultural Sciences.

[0038] 1.2 Method

[0039] 1.2.1 Breeding and preservation of materials

[0040] The germplasm resources of sweet potato are stored in resource nurseries in two ways: field and greenhouse.

[0041] 1.2.2 Genomic DNA extraction of sweet potato germplasm resources

[0042] In the sweet potato germplasm resource...

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Abstract

The invention provides a method for constructing and evaluating a sweet potato core germplasm resource library based on SSR (simple sequence repeat) molecule markers. The method includes the steps: firstly, extracting DNA (deoxyribonucleic acid) from tender leaves at the top ends of stored sweet potato germplasm resources; secondly, performing PCR amplification by screened polymorphism SSR primers; thirdly, recording banding pattern data of the germplasm resources according to amplification results; analyzing obtained data with the SSR markers of '0' and '1'; constructing sweet potato core germplasm by the aid of a locus preferred sampling method and Jaccard genetic distance according to the principle that germplasm sampled in an integral clustering manner can enter core germplasm; evaluating and confirming the resource library by the aid of phenotypic data. Amplification banding patterns of the SSR markers are clear, stable and good in repeatability. Compared with a method for constructing core germplasm based on phenotypic data of agronomic characters, the method for constructing sweet potato core germplasm based on molecule marker data has the advantages that the method is good in stability, time and labor are saved and the like.

Description

technical field [0001] The invention belongs to the technical field of plant germplasm resource evaluation, and in particular relates to a method for constructing a sweet potato core germplasm resource bank based on SSR molecular markers. Background technique [0002] Abundant genetic resources are the basis for various research work such as breeding of new varieties. However, due to the limited number of resources actually used in actual work, the large number of resources is not conducive to the comprehensive preservation and utilization of germplasm resources. For this reason, Frankle proposed the concept of core germplasm in 1984, that is, through scientific methods, select from the entire germplasm resources that part of the resources that can maximize the genetic diversity of the entire resource with the minimum number of resources and genetic repetition , in order to deeply evaluate and effectively protect and utilize the overall germplasm resources, and further deve...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李慧峰陈天渊黄咏梅李彦青吴翠荣滑金锋
Owner MAIZE RES INST GUANGXI ACADEMY OF AGRI SCI
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