Preparation method of upconversion luminescent nanomaterial micelles based on gadolinium ion doping

A technology of nanomaterials and gadolinium ions, applied in the field of preparation of upconversion luminescent nanomaterial micelles based on gadolinium ion doping, can solve the problem of low sensitivity, achieve high sensitivity magnetic resonance, good passive targeting ability, high optical Imaging the effect of dual modality imaging

Active Publication Date: 2019-01-29
SOUTHEAST UNIV
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Problems solved by technology

When used for MRI imaging, the tissue resolution and spatial resolution are ideal, but the sensitivity is relatively low

Method used

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: A preparation method of gadolinium-doped up-conversion luminescent nanomaterial micelles of the present invention that can be used as a magnetic resonance imaging contrast agent and can also realize optical imaging, the specific steps are as follows:

[0031] 1) Preparation of up-conversion luminescent nanomaterials:

[0032] Prepared by pyrolysis method: ① Weigh 0.72807g (2.4mmol) YCl respectively 3 ·6H 2 O, 0.290925g (0.54mmol) YbCl 3 ·6H 2 O and 0.022902g (0.06mmol) ErCl 3 ·6H 2 After O, transfer to a round-bottomed three-necked flask with a volume of 100ml, measure 45ml of oleic acid and 22.5ml of octadecene according to the volume ratio of 2:1, and heat the reaction system to 160°C under vigorous stirring with argon and maintain it for 30min; ② After naturally cooling to room temperature, add dropwise the solution containing 0.3g NaOH and 0.44445g 410ml of methanol solution of F, keep the temperature at 50°C and stir in airtight for 30min; ③Unde...

Embodiment 2

[0038] Accurately weigh 38.84mg DSPE-PEG2000 and 9mg mal-PEG-DSPE according to the molar ratio of 4:1, and add 6ml of chloroform, then ultrasonically disperse evenly, then add 0.5ml of chloroform-dispersed OA-UCNP@Gd, add 4ml of deionized water After the ultrasonic dispersion is uniform, use a rotary evaporator to evaporate excess chloroform at a rotation speed of 50 rpm at 65°C until it is clear and transparent. After the final reaction system is ultrasonicated with a probe for 5 minutes, it is transferred to an ultrafiltration tube and centrifuged at 5000g for 20 minutes to obtain UCNP@PEG .

Embodiment 3

[0040] Accurately weigh 54.0mg DSPE-PEG2000 and 9mg mal-PEG-DSPE according to the molar ratio of 6:1, and add 6ml of chloroform, then ultrasonically disperse evenly, then add 0.5ml of chloroform-dispersed OA-UCNP@Gd, add 4ml of deionized water After the ultrasonic dispersion is uniform, use a rotary evaporator to evaporate excess chloroform at a rotation speed of 50 rpm at 65°C until it is clear and transparent. After the final reaction system is ultrasonicated with a probe for 5 minutes, it is transferred to an ultrafiltration tube and centrifuged at 5000g for 20 minutes to obtain UCNP@PEG .

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Abstract

The invention discloses a preparation method for a micelle based on a gadolinium ion-doped up-conversion luminescent nano-material. The preparation method comprises the following steps: preparing the gadolinium ion-doped up-conversion luminescent nano-material by using an improved pyrolysis method; and preparing nano-micelle based on the gadolinium ion-doped up-conversion luminescent nano-material by using a rotary evaporation method. The preparation method provided by the invention is simple and easily practicable; a novel idea is provided for diagnosis and treatment of tumors on the basis of the advantage that the micelle integrates magnetic resonance imaging, optical imaging and the like into one; and the novel nanometer carrier overcomes the disadvantage of single-mode imaging in the prior art, can realizes synchronous in-vivo magnetic resonance imaging and optical imaging research and provides experimental research data and a theoretical base for clinical application and research.

Description

technical field [0001] The invention relates to a micelle based on an up-conversion luminescent nanomaterial, which can be used as a magnetic resonance imaging contrast agent and can realize optical imaging, and a preparation method thereof. Background technique [0002] Upconversion nanoparticles (UCNPs) can convert the absorbed low-energy near-infrared light into high-energy visible light. Appropriate surface modification of the nanomaterials makes them water-soluble, biocompatible and monodisperse. , is the prerequisite for its application in the biological field. Phospholipids are amphiphilic molecules whose hydrophilic ends are composed of phosphate-linked substituent groups and whose hydrophobic ends are composed of fatty acid hydrocarbon chains. When phospholipids are used to modify organic phase upconversion nanoluminescent materials, the fatty acid chains are bound to the surface of nanoparticles through hydrophobic interaction, while the hydrophilic ends face the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/12A61K49/00
CPCA61K49/0002A61K49/0013A61K49/126A61K49/1809
Inventor 安艳丽唐秋莎韩勇
Owner SOUTHEAST UNIV
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