Out-of-tube micro grafting method of bitter-gourd tissue culture seedlings

A technology of tissue cultured seedlings outside the test tube, which is applied in the field of micro-grafting outside the test tube of bitter gourd tissue cultured seedlings, which can solve the problems of high operational technical requirements and strict culture conditions, and achieve the effects of low cost, high survival rate, and less land occupation

Inactive Publication Date: 2017-12-26
广州市农业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Micro-grafting technology has been widely used in rapid detection of plant viruses, reproduction and preservation of precious breeding materials, etc., and has been applied to the research and production of various fruit trees such as jujube, citrus, apple, peach, grape, longan a

Method used

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  • Out-of-tube micro grafting method of bitter-gourd tissue culture seedlings
  • Out-of-tube micro grafting method of bitter-gourd tissue culture seedlings
  • Out-of-tube micro grafting method of bitter-gourd tissue culture seedlings

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Embodiment 1

[0039] A method for micro-grafting of bitter gourd tissue culture seedlings outside the test tube, the specific experimental method is:

[0040] (1) Cultivation of scion: select the top bud of a single plant with good comprehensive characters in the field (you can choose according to actual needs: such as drought-resistant, well-shaped melon, or heat-resistant plants, etc.) and have already formed the top buds of a single plant of balsam pear Or the lateral buds are explants, sterilized with 75% alcohol for 30 seconds, rinsed with sterile water three times, then sterilized with 0.01% mercury, and then inoculated into the induction medium MS+6-BA 0.3 mg / L for 20 days. After the apical buds or lateral buds germinate, cut the induced lateral buds and transfer them to the secondary culture medium MS+6-BA 0.2mg / L for continued cultivation; add sucrose 30g / L and agar 7g / L to the medium, pH 5.8 . During the subculture period, transfer once every 18-20 days on average to obtain a large ...

Embodiment 2

[0046] A method for micro-grafting of bitter gourd tissue culture seedlings outside the test tube, the specific experimental method is:

[0047] (1) Cultivation of scion: select the apical bud or lateral bud of a single plant of oilmelon variety with good comprehensive traits and fruited commercial melons in the field as explants, disinfect with 75% alcohol for 30 seconds, and rinse with sterile water four times After disinfection with 0.01% mercury, inoculate it on the induction medium MS+6-BA 0.4mg / L for 20 days. After the apical bud or lateral bud germinates, cut the induced lateral bud and transfer it to the secondary medium MS+ Continue to culture in 6-BA 0.3mg / L; add sucrose 40g / L and agar 7g / L to the medium, pH 5.8. During the subculture period, transfer once every 18-20 days on average to obtain a large number of rootless tissue culture seedlings as scion. Cultivation conditions: a closed culture room with artificial light, temperature (26±2)°C, relative humidity 70%-80%...

Embodiment 3

[0052] A method for micro-grafting of bitter gourd tissue culture seedlings outside the test tube, the specific experimental method is:

[0053] (1) Scion cultivation: select the terminal buds or lateral buds of individual plants of the pearl bitter gourd variety with good comprehensive traits and fruited commercial melons in the field as explants, sterilize with 75% alcohol for 30 seconds, and rinse with sterile water four times After disinfection with 0.015% mercury, inoculate it on the induction medium MS+6-BA0.5mg / L for 20 days. After the apical or lateral buds germinate, cut the induced lateral buds and transfer them to the secondary medium MS+ Continue to culture 6-BA 0.4mg / L; add sucrose 45g / L and agar 7g / L to the medium, pH 5.8. During the subculture period, transfer once every 18-20 days on average to obtain a large number of rootless tissue culture seedlings as scion. Cultivation conditions: a closed culture room with artificial light, temperature (26±2)°C, relative hu...

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Abstract

The invention discloses an out-of-tube micro grafting method of bitter-gourd tissue culture seedlings. The method includes the following steps of 1, culturing scions, wherein terminal buds or side buds which have good comprehensive characters and bear product bitter gourds in a field are selected to serve as explants, through induction culture and subculture, after natural-light seedling hardening, the obtained tissue culture seedlings are cut into stems which serve as the scions, and each stem has at least two buds; 2, culturing stocks, wherein towel-gourd seeds or pumpkin seeds are sowed in a disinfected medium, and after the seeds germinate, obtained seedlings serve as the stocks; 3, conducing grafting, wherein the scions obtained in the step 1 are grafted to the seedlings obtained in the step 2, and a 5%-8% (w/v) sucrose solution is sprayed every day till the scions survive; 4, conducting post-grafting management, wherein after being grafted, the scions are placed in a small arched greenhouse to be cultured till the scions sprout and each scion has two new leaves. The out-of-tube micro grafting method of the bitter-gourd tissue culture seedlings is short in cycle, low in cost, small in occupied space, high in survival rate and capable of greatly reducing the culture duration and cost of bitter gourds.

Description

Technical field [0001] The invention relates to the field of plant tissue culture technology and crop planting technology, in particular to a method for micro-grafting of bitter gourd tissue culture seedlings outside the test tube. Background technique [0002] In recent years, as the sales of bitter gourd on the market have increased year by year, the planting area has also been continuously expanded, and continuous cropping is inevitable in production, which has led to increasingly serious soil-borne diseases such as bitter gourd wilt and blight. The application of bitter gourd grafting cultivation technology in production has greatly reduced the mortality of cultivation, fundamentally overcomes the obstacle of continuous cropping and continuous cropping, and effectively prevented the damage of fusarium wilt. [0003] Micro-grafting is a technique for grafting tissue cultured scion and rootstock. It is a combination of plant tissue culture and grafting technique. There are two t...

Claims

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Application Information

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IPC IPC(8): A01G1/06A01H4/00
CPCA01H4/008A01G2/30
Inventor 吴蓓郑岩松戴修纯张木清陈胜文刘绍钦乔燕春
Owner 广州市农业科学研究院
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