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A method for identifying the age of pit mud

A technology of pit mud and identification instrument, which is applied in the field of wine making, can solve the problems of low accuracy, complicated detection process, and unobjectiveness, and achieve the effect of convenient viewing and management

Active Publication Date: 2020-11-20
SICHUAN JIANNANCHUN GRP CO LTD
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Problems solved by technology

This method has the defects of high subjectivity, complicated detection process, and low accuracy. However, the physical and chemical index values ​​can be achieved through simple adjustment of the process, and the artificial pit mud cultivation methods and formulas of different liquor production enterprises are not the same. Index detection and sensory identification are not objective

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  • A method for identifying the age of pit mud
  • A method for identifying the age of pit mud
  • A method for identifying the age of pit mud

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Embodiment Construction

[0026] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation manners of the present invention will now be described with reference to the accompanying drawings.

[0027] A method for identifying the age of a pit mud pit, comprising the following steps:

[0028] 1) Establishment of the dominant flora of functional gene microorganisms in pit mud

[0029] (1) Extraction of total RNA from pit mud samples. After adding Trizol to the pit mud sample at 10cm2 / ml, let it stand at room temperature for 5 minutes to fully lyse it. Centrifuge at 12000rpm for 5min and discard the precipitate. Add chloroform at 200ul chloroform / ml Trizol, shake and mix for 15 minutes, and place at room temperature for 15 minutes. Centrifuge at 4°C / 12000g for 15min. Aspirate the upper aqueous phase, transfer it to another centrifuge tube, add isopropanol to 0.5ml isopropanol / ml Trizol and mix well, place at room tempe...

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Abstract

The invention discloses a method for identifying a pit age of pit mud. According to the method, the automatic identification is realized by virtue of an automatic pit mud-pit age identification system based on cluster analysis of a metabolic fingerprint spectrum of a pit mud dominated microbial community, so that the operation is simple and convenient, the accuracy is high, the quality and maturity of the pit mud can be scientifically identified, and the scientific basis is provided for the ageing time of a new pit, the maintenance of an old pit and the like.

Description

technical field [0001] The invention belongs to the technical field of brewing, and in particular relates to a method for identifying the age of pit mud. Background technique [0002] Liquor is a precious heritage left over from the five thousand years of Chinese civilization and an important component of Chinese traditional culture. In the solid-state fermentation brewing process of Luzhou-flavor liquor, the cellar is the key equipment. "A thousand-year-old cellar is bad for ten thousand years, and good wine depends on the age of the cellar" shows that only a cellar with a long age can produce good wine. However, the identification of the age of the cellar is still based on sensory identification, and the corresponding physical and chemical indicators are detected at the same time. This method has the defects of high subjectivity, complicated detection process, and low accuracy. However, the physical and chemical index values ​​can be achieved through simple adjustment of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/04G16B40/00
CPCC12Q1/689G16B40/00
Inventor 唐清兰徐姿静刘孟华樊科权徐占成
Owner SICHUAN JIANNANCHUN GRP CO LTD
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