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Method for detecting flumazenil in Chinese patent medicines and health food by liquid chromatography-mass spectrometry

A flumazenil, LC/MS technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of accurate detection results, less interference and high sensitivity

Inactive Publication Date: 2018-04-24
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, drugs may be illegally added to memory-improving health foods, including flumazenil. Currently, there are few screening methods for the illegal addition of memory-improving traditional Chinese medicine preparations and health foods. Therefore, it is necessary to Flumazenil is detected in preparations and health foods to prevent and supervise the occurrence of adverse events caused by memory-improving Chinese patent medicines and health foods after entering the market to ensure safety. Rapid screening method for flumazenil in traditional Chinese medicine preparations and health food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Preparation of reference substance solution: take the flumazenil reference substance and dissolve it with methanol to prepare a flumazenil solution with a concentration of 1mg / ml, then take 1ml of the flumazenil solution in a measuring bottle, and dilute to 1 mg / ml with methanol 100ml to obtain the flumazenil reference substance solution.

[0021] (2) Prepare the sample solution to be tested: the configuration method of the liquid preparation: accurately draw a considerable oral dose, put it in a 50ml measuring bottle, add 20ml of 50% aqueous methanol solution, and ultrasonically process it for 15min at 1130W under the condition of 37kHz, let it cool, and use Dilute 50% aqueous methanol to the mark, shake well, take 1ml of the solution and dilute it 10 times with water, and use it as the test solution for later use.

[0022] The preparation method of the solid preparation: take the test product of the solid preparation and grind it finely, accurately weigh the dose ...

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Abstract

The invention discloses a method for detecting flumazenil in Chinese patent medicines and health food by liquid chromatography-mass spectrometry. The method comprises the following steps: (1) preparation of a reference substance: preparing the reference substance solution from a flumazenil reference substance and methanol; (2) preparation of a to-be-detected sample: extracting a Chinese materia medica preparation which improves memory in an assisted manner and health food with methanol, and purifying a solution obtained by extraction to obtain the to-be-detected sample solution; and (3) qualitatively and quantitatively measuring the flumazenil in a solution for testing product by using the reference substance solution and a liquid chromatograph / mass spectrometer on the to-be-detected sample solution. According to the method, detection is carried out by a multi-reaction monitoring mode; and the method is low in interference, high in sensitivity and accurate in detection result.

Description

technical field [0001] The invention relates to a detection method for flumazenil, in particular to a method for detecting flumazenil in Chinese patent medicines and health foods by liquid chromatography-mass spectrometry. Background technique [0002] Flumazenil has a nootropic effect, but it is mainly used for Meniere's syndrome, vascular headache and cerebral arteriosclerosis, and can be used for the treatment of acute ischemic cerebrovascular diseases, such as cerebral thrombosis, cerebral embolism, transient Cerebral insufficiency, etc.; also effective for orthostatic vertigo and tinnitus caused by high blood pressure. However, it can produce side effects such as loss of appetite, nausea, vomiting, dry mouth, headache, heart palpitations, and dermatitis. [0003] In recent years, with the increasing pressure of social competition and the expansion of an aging society, Chinese patent medicines and health care products that help improve memory have a considerable market ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 陈学松罗达龙刘慧妍
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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