Application of lncRNAGAS5 as age-related macular degeneration diagnostic marker

A technology of macular degeneration and markers, applied in the field of clinical diagnosis of molecular biology and medicine, can solve the problem of lack of specific biomarkers

Inactive Publication Date: 2018-05-01
常熟市第二人民医院
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Problems solved by technology

However, in addition to eye examination, there is still a lack of specific biomarkers for early AMD, so furth

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  • Application of lncRNAGAS5 as age-related macular degeneration diagnostic marker
  • Application of lncRNAGAS5 as age-related macular degeneration diagnostic marker
  • Application of lncRNAGAS5 as age-related macular degeneration diagnostic marker

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Embodiment 1

[0022] Example 1 lncRNA expression profile chip and expression verification analysis of early AMD patients

[0023] Part 1 Expression Spectrum Microarray Analysis

[0024] 1. Materials and methods

[0025] The data of this study comes from the Gene Expression Omnibus (GEO) public online database, its website is https: / / www.ncbi.nlm.nih.gov / geo / , and the GEO accession is GSE50195. There are 17 samples in this data, including 7 control samples, 9 early AMD samples.

[0026] 2. Data Analysis

[0027] 1. Differential lncRNA screening

[0028] First, combined with the chip platform (GPL17629) of GSE50195 data, add gene marker (gene symbol) and protein classification (Protein Classes) information to the downloaded data. Then, the limma package in the routine software R software in bioinformatics was used to screen the differential genes of 7 normal healthy control samples and 9 early AMD samples, and 7 control samples were used as the control group. The conditions for screening...

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Abstract

The invention relates to application of lncRNAGAS5 as an early stage age-related macular degeneration (AMD) diagnostic marker. Expression difference of lncRNAs between an early stage AMD patient and apatient in a control group is detected by virtue of a gene chip, growth arrest specific transcripts 5 (GAS5) are selected from all the differentially expressed lncRNAs for further verification, clinical sample capacity is expanded, circular lncRNA expression is carried out by adopting a fluorescent quantitative PCR method, contents of lncRNA in the AMD patient and the control group are compared,and feasibility of lncRNAGAS5 as the diagnostic marker is verified. ROC curve analysis indicates that area under the curve of GAS5 is 89.6%, so that GAS5 can be taken as the early stage age-related macular degeneration diagnostic marker.

Description

technical field [0001] The invention relates to the technical field of clinical diagnosis of molecular biology and medicine, specifically, the application of lncRNA as a diagnostic marker for early age-related macular degeneration. Background technique [0002] Age-related macular degeneration (AMD) is a common eye disease and the leading cause of blindness in the elderly. At present, anti-vascular endothelial growth factor (VEGF) or anti-angiogenic agents are mainly used to treat neovascular AMD clinically, but these treatments have a high recurrence rate and may cause serious side effects such as stroke. In addition, antagonists have been shown to be ineffective in some AMD patients. Therefore, early intervention of AMD to make it stay in the early stage of the disease can significantly improve the prognosis of patients' visual function. However, in addition to eye examination, there is still a lack of specific biomarkers for early AMD, so further research is needed to e...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 朱玮陆炯吴岩
Owner 常熟市第二人民医院
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