Determination method of multi-component content of Jade leaf golden flower

A kind of technology of jade leaf golden flower and determination method, which is applied in the direction of measuring device, material separation, analysis material, etc., can solve the problems such as content determination that have not yet been seen, and achieve the effects of good reproducibility, quality control and high precision

Inactive Publication Date: 2020-06-19
广西仙茱中药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Yuye Jinhua is recorded in the first volume of "Quality Standards of Zhuang Medicine of Guangxi Zhuang Autonomous Region". There are only physical and chemical identifications in the standards, and only TLC identifications in other patented medicines. There is no relevant report on its content determination.

Method used

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  • Determination method of multi-component content of Jade leaf golden flower
  • Determination method of multi-component content of Jade leaf golden flower
  • Determination method of multi-component content of Jade leaf golden flower

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] Embodiment, content is as follows:

[0023] (1) Chromatographic conditions: Octadecylsilane-bonded silica gel was used as filler; acetonitrile was used as mobile phase A, 0.1% phosphoric acid was used as mobile phase B, and gradient elution was carried out according to Table 1; the injection volume was 10 μL, and the column The temperature is 35°C, the flow rate is 1.0 mL / min, and the detection wavelength is 254nm;

[0024] Table 1 Gradient elution table of Yuyejinhua

[0025]

[0026] (2) Sample extraction: take about 0.5 g of the powder of this product (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 20 mL of 50% methanol, weigh it, and perform ultrasonic treatment (power 320 W , frequency 40 kHz) for 30 min, take it out, let it cool, weigh again, make up the lost weight with 50% methanol, shake well, filter, take the filtrate, and get it.

[0027] (3) Preparation of 9 reference substance stock solutions:...

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PUM

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Abstract

The invention discloses a determination method for multi-component contents of ramulus et radix mussaendae. The method is characterized by adopting HPLC and LC-MS for detecting the ramulus et radix mussaendae so as to build the method for simultaneously determining nine chemical components such as neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, shanzhiside methyl ester, 8-O-acetyl shanzhiside methyl ester, and ramulus et radix mussaendae nucleotide methyl ester in the ramulus et radix mussaendae. According to the method, through methodology validation, a quality inspection standard of the ramulus et radix mussaendae is perfected, and a foundation is laid for building the quality standard of the ramulus et radix mussaendae.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a multi-component content determination method of Jade Leaf Golden Flower. Background technique [0002] Jade leaf golden flower contains organic acid components (new chlorogenic acid, cryptochlorogenic acid, chlorogenic acid, 3,5- O -dicaffeoylquinic acid, 3,4- O -dicaffeoylquinic acid, 4,5- O -dicaffeoylquinic acid) and iridoid glycosides (shanzhiside methyl ester, 8- O -Acetyl shanzhiside methyl ester, japonica japonica acid methyl ester), and more saponins (such as japonica saponins R, V, M, Q, G, U, O). Among them, organic acid components and iridoid glycoside components are the main active components of Yuyejinhua. [0003] Yuyejinhua medicinal material is Rubiaceae plant Yuyejinhua Mussaenda pubescens Dried roots and stems of Ait. f. It can be harvested throughout the year, washed, cut into sections, and dried. It has the effects of cl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 谢培德张涛林雀跃黄莉莉黄清泉滕爱君唐秀玲
Owner 广西仙茱中药科技有限公司
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