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Method for improving chicken eimeria oocysts sporulation efficiency and application thereof

A chicken Emel ball and cystospore technology, which is applied in the field of veterinary biological products, can solve problems such as requirements and restrict production efficiency, and achieve the effects of improving production efficiency, improving sporulation efficiency, and reducing manpower and material resources.

Inactive Publication Date: 2018-06-01
TIANJIN HLINTE BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The original oocyst sporulation culture technology requires oocyst concentration, which greatly restricts its large-scale production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] Comparative Experiment:

[0019] Get 6 billion unspored Eimeria tenella oocysts that have just been harvested, and divide them into three groups equally, with 2 billion oocysts per group.

[0020] Group 1: Carry out sporulation culture according to the conventional sporulation technology, adjust the concentration of oocysts to 400,000 / ml with 0.2 chloramine T, a total of 5000 ml, pour it into a 2000ml beaker, the liquid level is 2cm, and need 25 2000ml beakers, Put them into a constant temperature shaking box for cultivation, the temperature is 28°C, and the rotation speed is 100r / min. After 48 hours, start to detect the sporulation rate, and end when the sporulation rate is ≥ 80%.

[0021] Group two carries out sporulation culture according to the method of the present invention, adjusts the concentration of oocysts to 5 million / ml with 0.2 chloramine T, totally 400 milliliters, pours it into a 2000ml beaker, the liquid level is not higher than 35cm, and needs one 200...

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Abstract

The invention relates to a novel method for improving chicken eimeria oocysts sporulation efficiency, in particular to a method for improving the chicken eimeria oocysts sporulation efficiency throughcontrolling the oocyst concentration, the liquid height and the oxygen concentration. The method has the advantages that the chicken eimeria oocysts sporulation efficiency can be greatly improved; the labor and material investment is reduced; the production efficiency is improved.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, in particular to a method for improving the sporulation efficiency of Eimeria gallinarum oocysts. Background technique [0002] In the production process of chicken coccidiosis vaccine, the sporulation culture of oocyst is a very critical link. The original oocyst sporulation culture technology requires oocyst concentration, which greatly restricts its large-scale production efficiency. In order to improve the sporulation efficiency of Eimeria gallinarum oocysts, researchers have conducted a large number of experimental studies. Contents of the invention [0003] The object of the present invention is to provide a new method for improving the sporulation efficiency of Eimeria gallinarum oocysts, the method can greatly improve the sporulation efficiency of Eimeria gallinarum oocysts and improve production efficiency. [0004] The present invention is achieved through the following...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/10C12N3/00C12R1/90
CPCC12N1/10C12N3/00
Inventor 安同伟史晓华赵金羽
Owner TIANJIN HLINTE BIOTECH CO LTD
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