32 results about "Eimeria gilruthi" patented technology

The invention relates to a Chinese traditional medicine granule with the efficacies of expelling worms, protecting the liver and stopping bleeding and dysentery, including ten Chinese traditional medicines of southernwood, agrimony, dichroa febrifuga, garden burnet root, pulsatilla chinensis, golden buckwheat rhizome, thistle, white paeony root, radix astragali and liquorice. The invention is mainly used for treating the symptoms of intestinal tract bleeding, intestinal wall thickening and necrosis and intestinal mucosa shedding, etc. caused by chicken eimeria maxima, eimeria acervulina, eimeria necatrix and so on. The invention can alleviated the symptoms of dull standing, aphagia or anorexia, defecating bloody stool or whole blood, emaciation and poor growth of sick chickens caused by coccidiosis. The invention has the efficacies of expelling worms, protecting the liver and stopping bleeding and dysentery. By considering the short avian genital tract and the special physiological structure of inability to digest lignocellulose, the medicine is prepared into soluble granules which can quickly be dissolved into drinking water which is absorbed by sick chickens so as to achieve the purpose of quick effect.

The present invention relates to a composition for improving the yield of Eimeria maxima oocysts, and a preparation method thereof, wherein the composition comprises, by weight, 10-30 parts of pulsatilla chinensis, 1-10 parts of vitamin A, 0.5-10 parts of vitamin E, 2-30 parts of berberine, 0.5-5 parts of sodium cholate, and 10-90 parts of stone powder. According to the present invention, the composition has characteristics of scientific and reasonable formula and simple process, can improve the yield of Eimeria maxima oocysts by maintaining the integrity of the intestinal tract epithelium mucous membrane, reducing the damage of intestinal tract gram-negative bacteria on the intestinal tract epithelial cells, improving the excystation rate of sporozoites, and other effects, and can improve the production efficiency of the Eimeria maxima vaccine, reduce the production cost, and save the social resources.

The invention provides a feed for treating eimeria tenella infection. The feed comprises the following raw materials: wheat, bean pulp, bone meal, rapeseed meal, monocalcium phosphate, vitamin E, vitamin K, pantothenic acid, lysine, methionine, threonine and a traditional Chinese medicine preparation for treating eimeria tenella coccidiosis. The traditional Chinese medicine preparation contained in the feed is low in drug resistance and generates few drug residues, has no toxic and side effect and has a good therapeutic effect of eimeria tenella coccidiosis.

The invention discloses a preparation method of coccidiosis in chicken obtained by ultraviolet ray and cobalt60gamma-ray irradiation as well as precocity breeding. The preparation method comprises: sampling; propagating oocysts; irradiating the offspring oocysts with ultraviolet rays and cobalt60gamma-rays; determining the dosage of ultraviolet ray and cobalt60gamma-ray irradiation; obtaining seven types of Eimeria (Eimeria tenella, Eimeria acervulina, Eimeria maxima, Eimeria necatrix, Eimeria brunette, Eimeria mitis, and precocious Eimeria) precocious strain oocysts; taking the Eimeria oocysts processed by ultraviolet ray and cobalt60gamma-ray irradiation in steps 3 as the parent to carry out coccidiosis in chicken precocity breeding; and blending. The method can greatly shorten the timefor precocity breeding and improve breeding efficiency, thus obtaining plentiful commercialized precocity attenuated Eimeria strains in a short time.

The invention aims to disclose a nested PCR (polymerase chain reaction) detection kit for 11 types of rabbit Eimeria. The nested PCR detection kit is characterized in that with an ITS-1 sequence of 18S rRNA gene of the Rabbit Eimeria as a target sequence, a pair of genus-specific nested PCR primers are designed, 11 pairs of species-specific nested PCR primers are designed for each Eimeria body, and a nested PCR detection method is established and used for the detection of 11 types of rabbit Eimeria in a clinical fecal sample. The nested PCR detection kit is rigorous in design, easy to operate, high in flexibility, high in specificity, convenient to operate in batch, capable of identifying infections of specific types of rabbit Eimeria and accurate and objective in result judgment.

The invention discloses an egg yolk antibody and a preparation method and device thereof. A test object is used for performing polypide reinvigoration and proliferation on tender eimeriida, giant eimeriida and poisoned eimeriida, after the insects are mixed in equal proportions, antigen is prepared by ultrasonic disruption, the obtained antigen solution is mixed with an equal volume of freund's complete adjuvant and freund's incomplete adjuvant separately, laying hens are immunized three times, egg yolk antibodies in collected egg yolk after three times of immunizationare extracted, purified and identified, and the antibody titer is evaluated; the research on different yolk powder preparation processes is carried out, the effect of lyophilized powder preparation is significantly higher than that of powder spraying technology, the matchingrelationship between the yolk and the dilution, the lyophilization temperature curve and other parameter conditions in the process are prepared, the cost is relatively low, the preparation efficiency is high, antibody loss rate is only 10%, high antibody titer, antibody titer after high-temperature powder spraying shows a high downward trend, and the method is obviously superior to the high-temperature powder spraying technology for preparing yolk powder.

The invention relates to the field of poultry breeding and specifically relates to a method for accurately estimating each index concentration after challenge of chicken eimeria tenella according to resistance indexes measured after immunization with a chicken coccidiosis vaccine. The method comprises the steps of immunizing samples to be screened with the chicken coccidiosis vaccine and then measuring five resistance indexes in the blood plasma, namely CAT, SOD, GSH-Px, MDA and IFN-gamma, and then accurately estimating the corresponding concentration of each index after the challenge (infection) of the chicken eimeria tenella by use of a pre-established regression equation of each resistance index according to the five resistance indexes measured after immunization with the chicken coccidiosis vaccine so as to select chicken eimeria tenella resistant individuals by use of the five resistance indexes under the condition of non-challenge (infection), wherein the regression equation of the resistance index refers to the quantitative relation of the resistance index after immunization with the chicken coccidiosis vaccine and challenge (infection). The method is capable of realizing conventional selective breeding of the chicken coccidiosis, and is safe and significant.

The invention relates to the field of gene engineering and provides an eimeria tenella microneme protein-2 mutant EtMIC2-1130 of chickens. The mutant is obtained as follows: in-vitro orthogenesis is performed with EtMIC2 (GenBank:KC333870.1) of wild eimeria tenella SD-01 as basic protein, a gene mutation library is established, yeast is used for surface display of a protein mutant library, altered protein with immunogenicity and immune protection remarkably improved is screened by means of sorting by a flow cytometry, animal immune protection effect evaluation and the like, and the mutant is obtained finally. The mutant altered protein has six amino acid mutation sites; the immune protection effect ACI of altered EtMIC2 is improved by about 21.94% while compared with that of natural EtMIC2, and the mutant can serve as a vaccine to be applied in the field of resistance to infection by eimeria tenella of the chickens.

The invention relates to a traditional Chinese medicine composition for resisting eimeria tenella, which comprises the following raw medicines: kuh-seng, dichroa root, polygonum aviculare, chinaberry bark, pulsatilla chinensis, sealwort and white peony root. According to the Chinese veterinary medicine theory, the clinical characters of coccidiosis and the characteristics of chicken diseases, the invention develops a traditional Chinese medicine composition for resisting eimeria tenella. The traditional Chinese medicine composition has the prescription principles of mainly clearing away heat and drying dampness as well as cooling the blood to stop bleeding and reinforcing deficiency in an auxiliary way, is prepared by screening, and has an obvious curative effect on chicken coccidiosis through actual use.

The invention belongs to the technical field of parasite molecular detection, and relates to a molecular detection method of sulfanilamide drug-resistant eimeria tenella, in particular to a method fordetecting sulfanilamide drug-resistant eimeria tenella by using a four-primer amplification refractory system-PCR. According to the invention, two specific primer pairs are designed according to thedhps gene sequence of the eimeria tenella; the nucleotide sequences of the primer pair are shown as SEQ ID NO: 2 to SEQ ID NO: 5 respectively, then total DNA of a sample is extracted, an ARMS-PCR amplification reaction is carried out, and finally whether the sample contains a sulfanilamide drug-resistant insect strain or not is determined through agarose gel electrophoresis. The method is high insensitivity and strong in specificity, and can be used for quickly identifying the sulfanilamide drug-resistant eimeria tenella.

The invention provides a drug for treating chicken Eimeria tenella. The drug comprises crude medicines such as antifebrile dichroa, rheum officinale, caulis spatholobi, sealwort, aleppo avens, madder, golden cypress, uncaria, agrimonia pilosa, radix pseudostellariae, dogwood, brucea javanica, savatier monochasma herbs, trollflower, elephantopus scaber, ginseng leaves, resina toxicodendri, lonicerae flos, szechwan chinaberry fruits, purslane, cortex acanthopanacis, spina gleditsiae, rabdosia rubescens, fructus kochiae, prepared rehmannia roots, cortex phellodendri amurense and liquorice. The drug is not prone to development of drug resistance and extremely small in toxic and side effects and has the advantages of capabilities of promoting the organism growth, enhancing the immunity, improving the disease resistance of organisms and the like, the purpose of coccidiosis prevention can be achieved, the problem of drug residues which are harmful to human bodies and hinder export of broiler chickens can be solved, in addition, the immunity of the organisms can be enhanced, the growth and the development of the chickens can be promoted, and the productivity of the chickens can be improved.

The invention relates to the field of gene engineering and provides an eimeria tenella microneme protein-2 mutant EtMIC2-2119 of chickens. The mutant is obtained as follows: in-vitro orthogenesis is performed with EtMIC2 (GenBank:KC333870.1) of wild eimeria tenella SD-01 as basic protein, a gene mutation library is established, yeast is used for surface display of a protein mutant library, altered protein with immunogenicity and immune protection remarkably improved is screened by means of sorting by a flow cytometry, animal immune protection effect evaluation and the like, and the mutant is obtained finally. The mutant altered protein has 11 amino acid mutation sites; the immune protection effect ACI of altered EtMIC2 is improved by about 25.06% while compared with that of natural EtMIC2, and the mutant can serve as a vaccine to be applied in the field of resistance to infection by eimeria tenella of the chickens.

The invention relates to the field of disease-resistant breeding of poultry, in particular to a selective breeding method by comprehensive selection indexes of resistance of Eimeria tenella of chickens. The selective breeding method includes feeding a certain quantity of eimeria tenella spore oocysts of each chicken some-day old, measuring incremental weight within 3-5 days after infection and concentration of CAT (catalase), SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), MDA (malondialdehyde) and IFN-gamma (interferon-gamma) in blood plasma in eighth day after infection, converting the six indexes into standard Z scores, and substituting into a comprehensive selection index formula to calculate comprehensive selection index values of each chicken, and selecting to reserve resistant chicken individuals according to the values.

The invention discloses a composition for treating fowl enterovirus syndrome and a preparation method thereof, which aim at providing a composition with a good curative effect on integrally treating the fowl enterovirus syndrome and the preparation method thereof. The composition consists of the following ingredients in percentage by weight: 5 to 20 percent of sulfaquinoxaline sodium, 5 to 15 percent of ciprofloxacin hydrochloride, 1 to 4 percent of trimethoprim lactate, 1 to 5 percent of vitamin K3, 5 to 20 percent of sodium bicarbonate and the balance anhydrous dextrose. The adopted sulfaquinoxaline sodium has the strong effects on giant eimeria, eimeria brunette, eimeria acervulina and the like, and the synergia effect of the trimethoprim lactate is used as the auxiliary, so the eimeria resistance capability is greatly enhanced. The ciprofloxacin hydrochloride has the strong force on the bacterial infection in the enterovirus syndrome, and the killing effect is realized. The two effects are combined, and the pathogen causing the enterovirus syndrome can be targeted to be killed. The vitamin K3 can be used for controlling the intestinal tract bleeding caused by the enterovirus syndrome and reducing the side effect caused by the intestinal tract bleeding. The sodium bicarbonate can be used for reducing the kidney injury to the fowl caused by the sulfaquinoxaline sodium.

The invention relates to a novel method for improving chicken eimeria oocysts sporulation efficiency, in particular to a method for improving the chicken eimeria oocysts sporulation efficiency throughcontrolling the oocyst concentration, the liquid height and the oxygen concentration. The method has the advantages that the chicken eimeria oocysts sporulation efficiency can be greatly improved; the labor and material investment is reduced; the production efficiency is improved.

The invention relates to a traditional Chinese medicine composition for preventing and treating chicken coccidiosis as well as a preparation method, a use method and application of the traditional Chinese medicine composition and belongs to the technical field of veterinary traditional Chinese medicines. The traditional Chinese medicine composition is composed of the following components in partsby mass: 30-50 parts of herba verbenae, 15-25 parts of cortex phellodendri, 15-30 parts of gardenia jasminoides ellis, 10-25 parts of rhizoma imperatae and 5-15 parts of rhizoma atractylodis Macrocephalae. The traditional Chinese medicine composition is scientifically formulated according to a basic principle of compatibility in traditional Chinese medicine, so that the finally prepared traditional Chinese medicine composition has good curative effect on damp-heat syndrome, dysfunction in spleen transportation and transformation and other clinical symptoms caused by E. tenella, and does not affect later product safety.