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32 results about "Eimeria gilruthi" patented technology
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Nine of 23 (39%) research ewes with severe diarrhea and weight loss had histologic lesions consistent with Eimeria gilruthi infection in their abomasa. Gross anatomic lesions included hundreds of opaque 1-mm nodules in abomasal mucosa that corresponded microscopically to 200–300 µm diameter organisms surrounded by areas of necrosis.
Probiotics as alternative medicines against infectious diseases
A novel representational system that is a method for improving feeding probiotics is provided. The probiotics, especially the Pediococcus acidilactici and the Saccharomyces boulardii are mixed in different colloid net including xanthan, chitosan or gelatin. By using said mixed colloid the activity of the probiotics can be protected, and multiple execrable surroundings and disadvantageous temperature for growth can be prevented. The novel representational method also provides many kinds of method for treating infectious disease for birds and mammals by using activated probiotics. Furthermore, the probiotics is usually acted as adjuvants for improving the generation of antibody and the cellularity immunity effect. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.
Owner:MEDICAL TECHNIC CORP
Chinese medicinal granule with worm-expelling liver-caring, hemostasis and dysentery-stopping efficacy
InactiveCN101209296AHas anti-worm and liver protectionWith hemostasis and dysenteryDigestive systemAntiparasitic agentsPolygonum fagopyrumFeces
The invention relates to a Chinese traditional medicine granule with the efficacies of expelling worms, protecting the liver and stopping bleeding and dysentery, including ten Chinese traditional medicines of southernwood, agrimony, dichroa febrifuga, garden burnet root, pulsatilla chinensis, golden buckwheat rhizome, thistle, white paeony root, radix astragali and liquorice. The invention is mainly used for treating the symptoms of intestinal tract bleeding, intestinal wall thickening and necrosis and intestinal mucosa shedding, etc. caused by chicken eimeria maxima, eimeria acervulina, eimeria necatrix and so on. The invention can alleviated the symptoms of dull standing, aphagia or anorexia, defecating bloody stool or whole blood, emaciation and poor growth of sick chickens caused by coccidiosis. The invention has the efficacies of expelling worms, protecting the liver and stopping bleeding and dysentery. By considering the short avian genital tract and the special physiological structure of inability to digest lignocellulose, the medicine is prepared into soluble granules which can quickly be dissolved into drinking water which is absorbed by sick chickens so as to achieve the purpose of quick effect.
Owner:TIANJIN RINGPU BIO TECH
Composition for improving yield of Eimeria maxima oocysts, and preparation method thereof
ActiveCN107019111AIncrease productionScientific and reasonable compositionAnimal feeding stuffAccessory food factorsEpitheliumBerberine
The present invention relates to a composition for improving the yield of Eimeria maxima oocysts, and a preparation method thereof, wherein the composition comprises, by weight, 10-30 parts of pulsatilla chinensis, 1-10 parts of vitamin A, 0.5-10 parts of vitamin E, 2-30 parts of berberine, 0.5-5 parts of sodium cholate, and 10-90 parts of stone powder. According to the present invention, the composition has characteristics of scientific and reasonable formula and simple process, can improve the yield of Eimeria maxima oocysts by maintaining the integrity of the intestinal tract epithelium mucous membrane, reducing the damage of intestinal tract gram-negative bacteria on the intestinal tract epithelial cells, improving the excystation rate of sporozoites, and other effects, and can improve the production efficiency of the Eimeria maxima vaccine, reduce the production cost, and save the social resources.
Owner:TIANJIN HLINTE BIOTECH CO LTD
Method for preparing chicken coccidiosis high immunity yolk antibody
InactiveCN101186647AChange the concept of prevention and treatmentAvoid residueEgg immunoglobulinsYolkCoccidiosis
The invention relates to a preparation method of chicken coccidia hyperimmune egg yolk antigen, which comprises that (1) extracting and purifying spore reproductive stage antigen, merozoite reproductive stage antigen, and gamete reproductive stage antigen at each growth stage of chicken coccidian, (2), mixing the three antigens in a certain ratio, selecting suitable immunity adjuvant to be mixed with the antigens to prepare a mixture antigen, (3), selecting the laying hen without immunity, injecting the mixture antigens prepared by different immunity adjuvant into the muscles of thorax of the immunity chicken, (4), collecting the eggs of the immunity chickens, separating yolk, separating and purifying yolk antibody via water dilution, testing the drug effect of the antibody. The protective rates on E. tenella coccidiosis and E. necatrix coccidiosis respectively reach 100% and 96.7% by clinical test, which anti-coccidia indexes are 184.8 and 165.4. The successful industrialization of the inventive product provides a new route to prevent coccidiosis, and provides the base for resolving drug residue or the like.
Owner:CHENGDU QIANKUN VETERINARY PHARMA
Feed for treating eimeria tenella infection
ActiveCN103520605ADelay drug resistanceReduce drug residuesAnimal feeding stuffAntiparasitic agentsCoccidiosisSide effect
The invention provides a feed for treating eimeria tenella infection. The feed comprises the following raw materials: wheat, bean pulp, bone meal, rapeseed meal, monocalcium phosphate, vitamin E, vitamin K, pantothenic acid, lysine, methionine, threonine and a traditional Chinese medicine preparation for treating eimeria tenella coccidiosis. The traditional Chinese medicine preparation contained in the feed is low in drug resistance and generates few drug residues, has no toxic and side effect and has a good therapeutic effect of eimeria tenella coccidiosis.
Owner:潍坊新希望六和饲料科技有限公司
Preparation method of coccidiosis in chicken obtained by ultraviolet ray and cobalt60gamma-ray irradiation as well as precocity breeding
InactiveCN101642566AImprove breeding efficiencyAntiparasitic agentsAntibody medical ingredientsEimeria maximaUltraviolet
The invention discloses a preparation method of coccidiosis in chicken obtained by ultraviolet ray and cobalt60gamma-ray irradiation as well as precocity breeding. The preparation method comprises: sampling; propagating oocysts; irradiating the offspring oocysts with ultraviolet rays and cobalt60gamma-rays; determining the dosage of ultraviolet ray and cobalt60gamma-ray irradiation; obtaining seven types of Eimeria (Eimeria tenella, Eimeria acervulina, Eimeria maxima, Eimeria necatrix, Eimeria brunette, Eimeria mitis, and precocious Eimeria) precocious strain oocysts; taking the Eimeria oocysts processed by ultraviolet ray and cobalt60gamma-ray irradiation in steps 3 as the parent to carry out coccidiosis in chicken precocity breeding; and blending. The method can greatly shorten the timefor precocity breeding and improve breeding efficiency, thus obtaining plentiful commercialized precocity attenuated Eimeria strains in a short time.
Owner:QINGDAO SHENGNUOWEITE BIOTECH
Nested PCR (polymerase chain reaction) detection kit for 11 types of rabbit Eimeria
InactiveCN106319046AImprove extraction efficiencyIncreased sensitivityMicrobiological testing/measurementEimeriaBiology
The invention aims to disclose a nested PCR (polymerase chain reaction) detection kit for 11 types of rabbit Eimeria. The nested PCR detection kit is characterized in that with an ITS-1 sequence of 18S rRNA gene of the Rabbit Eimeria as a target sequence, a pair of genus-specific nested PCR primers are designed, 11 pairs of species-specific nested PCR primers are designed for each Eimeria body, and a nested PCR detection method is established and used for the detection of 11 types of rabbit Eimeria in a clinical fecal sample. The nested PCR detection kit is rigorous in design, easy to operate, high in flexibility, high in specificity, convenient to operate in batch, capable of identifying infections of specific types of rabbit Eimeria and accurate and objective in result judgment.
Owner:JILIN UNIV
Egg yolk antibody for anti-chicken-coccidiosis and preparation method and device thereof
The invention discloses an egg yolk antibody and a preparation method and device thereof. A test object is used for performing polypide reinvigoration and proliferation on tender eimeriida, giant eimeriida and poisoned eimeriida, after the insects are mixed in equal proportions, antigen is prepared by ultrasonic disruption, the obtained antigen solution is mixed with an equal volume of freund's complete adjuvant and freund's incomplete adjuvant separately, laying hens are immunized three times, egg yolk antibodies in collected egg yolk after three times of immunizationare extracted, purified and identified, and the antibody titer is evaluated; the research on different yolk powder preparation processes is carried out, the effect of lyophilized powder preparation is significantly higher than that of powder spraying technology, the matchingrelationship between the yolk and the dilution, the lyophilization temperature curve and other parameter conditions in the process are prepared, the cost is relatively low, the preparation efficiency is high, antibody loss rate is only 10%, high antibody titer, antibody titer after high-temperature powder spraying shows a high downward trend, and the method is obviously superior to the high-temperature powder spraying technology for preparing yolk powder.
Owner:徐前明
Resistance index estimation method for selective breeding of chicken eimeria tenella resistant individuals
InactiveCN104255657AMethod securityOther apparatusTaming and training devicesEstimation methodsBlood plasma
The invention relates to the field of poultry breeding and specifically relates to a method for accurately estimating each index concentration after challenge of chicken eimeria tenella according to resistance indexes measured after immunization with a chicken coccidiosis vaccine. The method comprises the steps of immunizing samples to be screened with the chicken coccidiosis vaccine and then measuring five resistance indexes in the blood plasma, namely CAT, SOD, GSH-Px, MDA and IFN-gamma, and then accurately estimating the corresponding concentration of each index after the challenge (infection) of the chicken eimeria tenella by use of a pre-established regression equation of each resistance index according to the five resistance indexes measured after immunization with the chicken coccidiosis vaccine so as to select chicken eimeria tenella resistant individuals by use of the five resistance indexes under the condition of non-challenge (infection), wherein the regression equation of the resistance index refers to the quantitative relation of the resistance index after immunization with the chicken coccidiosis vaccine and challenge (infection). The method is capable of realizing conventional selective breeding of the chicken coccidiosis, and is safe and significant.
Owner:YANGZHOU UNIV
Composition capable of increasing yield of eimeria tenella coccidian oocysts and preparation method thereof
InactiveCN108148761AIncrease productionScientific and reasonable compositionProtozoaMicroorganism based processesPropolisAnaerobic bacteria
The invention relates to a composition capable of increasing yield of eimeria tenella coccidian oocysts and a preparation method thereof. The composition is composed of, by weight, 30-50 parts of cortex phellodendri, 0.5-5 parts of propolis, 20-60 parts of coptis chinensis and 10-20 parts of metronidazole. The composition is scientific and reasonable, simple in process, convenient to use and low in cost. The composition can increase yield of eimeria tenella coccidian oocysts through effects of preventing and treating intestinal inflammation, inhibiting breeding of anaerobic bacteria in cecum,promoting tissue cell regeneration and accelerating intestinal mucosa repair, reduce the number of chicks used for oocyst propagation and lower manpower, material resource and time cost, thereby greatly improving production efficiency of avian coccidiosis vaccines.
Owner:TIANJIN HLINTE BIOTECH CO LTD
Eimeria tenella microneme protein-2 mutant EtMIC2-1130 of chickens
The invention relates to the field of gene engineering and provides an eimeria tenella microneme protein-2 mutant EtMIC2-1130 of chickens. The mutant is obtained as follows: in-vitro orthogenesis is performed with EtMIC2 (GenBank:KC333870.1) of wild eimeria tenella SD-01 as basic protein, a gene mutation library is established, yeast is used for surface display of a protein mutant library, altered protein with immunogenicity and immune protection remarkably improved is screened by means of sorting by a flow cytometry, animal immune protection effect evaluation and the like, and the mutant is obtained finally. The mutant altered protein has six amino acid mutation sites; the immune protection effect ACI of altered EtMIC2 is improved by about 21.94% while compared with that of natural EtMIC2, and the mutant can serve as a vaccine to be applied in the field of resistance to infection by eimeria tenella of the chickens.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Traditional Chinese medicine composition for resisting eimeria tenella
ActiveCN105031245AGood prevention effectGood treatment effectDigestive systemAntiparasitic agentsCoccidiosisDisease
The invention relates to a traditional Chinese medicine composition for resisting eimeria tenella, which comprises the following raw medicines: kuh-seng, dichroa root, polygonum aviculare, chinaberry bark, pulsatilla chinensis, sealwort and white peony root. According to the Chinese veterinary medicine theory, the clinical characters of coccidiosis and the characteristics of chicken diseases, the invention develops a traditional Chinese medicine composition for resisting eimeria tenella. The traditional Chinese medicine composition has the prescription principles of mainly clearing away heat and drying dampness as well as cooling the blood to stop bleeding and reinforcing deficiency in an auxiliary way, is prepared by screening, and has an obvious curative effect on chicken coccidiosis through actual use.
Owner:SHANDONG ENKANG PHARMA
ARMS-PCR primer of sulfanilamide drug-resistant eimeria tenella and molecular detection method thereof
InactiveCN110484640AFast wayMethod specificMicrobiological testing/measurementMicroorganism based processesNucleotideSulfanilamide
The invention belongs to the technical field of parasite molecular detection, and relates to a molecular detection method of sulfanilamide drug-resistant eimeria tenella, in particular to a method fordetecting sulfanilamide drug-resistant eimeria tenella by using a four-primer amplification refractory system-PCR. According to the invention, two specific primer pairs are designed according to thedhps gene sequence of the eimeria tenella; the nucleotide sequences of the primer pair are shown as SEQ ID NO: 2 to SEQ ID NO: 5 respectively, then total DNA of a sample is extracted, an ARMS-PCR amplification reaction is carried out, and finally whether the sample contains a sulfanilamide drug-resistant insect strain or not is determined through agarose gel electrophoresis. The method is high insensitivity and strong in specificity, and can be used for quickly identifying the sulfanilamide drug-resistant eimeria tenella.
Owner:HUAZHONG AGRI UNIV
Hyperimmunized egg product for treatment of necrotic enteritis in poultry
In one aspect, the present invention is directed to a method for preventing or treating necrotic enteritis by administering a hyperimmunized egg product obtained from an egg-producing animal to an avian. The hyperimmunized egg product may contain an antibody specific to an antigen selected from the group consisting of Clostridium perfringens α-toxin, Clostridium perfringens elongation factor Tu (EF-Tu), Clostridium perfringens necrotic enteritis B-like (NetB) toxin, Clostridium perfringens Pyruvate: Ferredoxin oxidoreductase (PFO), and Eimeria tenella elongation factor 1-alpha.
Owner:ARKION LIFE SCI LLC +1
Drug for treating chicken Eimeria tenella
InactiveCN103721046ALower resistanceSmall side effectsAnimal feeding stuffAntiparasitic agentsCoccidiosisDisease
The invention provides a drug for treating chicken Eimeria tenella. The drug comprises crude medicines such as antifebrile dichroa, rheum officinale, caulis spatholobi, sealwort, aleppo avens, madder, golden cypress, uncaria, agrimonia pilosa, radix pseudostellariae, dogwood, brucea javanica, savatier monochasma herbs, trollflower, elephantopus scaber, ginseng leaves, resina toxicodendri, lonicerae flos, szechwan chinaberry fruits, purslane, cortex acanthopanacis, spina gleditsiae, rabdosia rubescens, fructus kochiae, prepared rehmannia roots, cortex phellodendri amurense and liquorice. The drug is not prone to development of drug resistance and extremely small in toxic and side effects and has the advantages of capabilities of promoting the organism growth, enhancing the immunity, improving the disease resistance of organisms and the like, the purpose of coccidiosis prevention can be achieved, the problem of drug residues which are harmful to human bodies and hinder export of broiler chickens can be solved, in addition, the immunity of the organisms can be enhanced, the growth and the development of the chickens can be promoted, and the productivity of the chickens can be improved.
Owner:QINGDAO HUAREN TECH INCUBATOR
Eimeria tenella microneme protein-2 mutant EtMIC2-2119 of chickens
InactiveCN106046136AGood immune protectionDepsipeptidesAntiparasitic agentsSurface displayADAMTS Proteins
The invention relates to the field of gene engineering and provides an eimeria tenella microneme protein-2 mutant EtMIC2-2119 of chickens. The mutant is obtained as follows: in-vitro orthogenesis is performed with EtMIC2 (GenBank:KC333870.1) of wild eimeria tenella SD-01 as basic protein, a gene mutation library is established, yeast is used for surface display of a protein mutant library, altered protein with immunogenicity and immune protection remarkably improved is screened by means of sorting by a flow cytometry, animal immune protection effect evaluation and the like, and the mutant is obtained finally. The mutant altered protein has 11 amino acid mutation sites; the immune protection effect ACI of altered EtMIC2 is improved by about 25.06% while compared with that of natural EtMIC2, and the mutant can serve as a vaccine to be applied in the field of resistance to infection by eimeria tenella of the chickens.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Selective breeding method by comprehensive selection indexes of resistance of Eimeria tenella of chickens
InactiveCN104221995AGood selective breedingReduce labor intensityAnimal husbandrySporeMalondialdehyde
The invention relates to the field of disease-resistant breeding of poultry, in particular to a selective breeding method by comprehensive selection indexes of resistance of Eimeria tenella of chickens. The selective breeding method includes feeding a certain quantity of eimeria tenella spore oocysts of each chicken some-day old, measuring incremental weight within 3-5 days after infection and concentration of CAT (catalase), SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), MDA (malondialdehyde) and IFN-gamma (interferon-gamma) in blood plasma in eighth day after infection, converting the six indexes into standard Z scores, and substituting into a comprehensive selection index formula to calculate comprehensive selection index values of each chicken, and selecting to reserve resistant chicken individuals according to the values.
Owner:YANGZHOU UNIV
A kind of traditional Chinese medicine composition for poultry prevention and treatment of coccidiosis and preparation method thereof
ActiveCN104027420BPreserve the combination effectDefinite curative effectAntibacterial agentsAntiparasitic agentsCoccidiosisHydrolysate
Owner:SRICK TIANJIN BIO TECH
Feed for the treatment of Eimeria tenella infection
ActiveCN103520605BDelay drug resistanceReduce drug residuesAccessory food factorsAntiparasitic agentsCoccidiosisSide effect
Owner:潍坊新希望六和饲料科技有限公司
Traditional Chinese medicine composition for treating chicken eimeria tenella coccidiosis
InactiveCN105343524ALower resistanceSmall side effectsMetabolism disorderAnimal feeding stuffCoccidiosisDisease
The invention provides a traditional Chinese medicine composition for treating chicken eimeria tenella coccidiosis. The traditional Chinese medicine composition is prepared from antifebrile dichroa, rheum officinale, caulis spatholobi, rhizoma polygonati, Aleppo avens, rubia cordifolia, cortex phellodendri, uncaria, hairyvein agrimony, radix pseudostellariae, dogwood, brucea javanica, savatier monochasma herb, trollflower, elephantopus scaber, Szechwan chinaberry fruits, portulaca oleracea, cortex acanthopanacis, spina gleditsiae, rabdosia rubescens, fructus kochiae, prepared rehmannia roots, cortex phellodendri chinensis and liquorice. The traditional Chinese medicine composition is not likely to generate resistance and extremely small in toxic and side effect, and further has the advantages of promoting organism growth, enhancing immunity, improving organism disease resistance and the like; the purpose of preventing and treating coccidiosis can be achieved, and the problems of medicine residues harming the human body and obstructing broiler chicken export can be solved; meanwhile, the organism immunity can be enhanced, growth and development of chickens are promoted, and the productivity of the chickens is improved.
Owner:殷志杰
Composition for treating fowl enterovirus syndrome and preparation method thereof
InactiveCN102406655AImprove abilitiesControl bleedingAntibacterial agentsOrganic active ingredientsSodium bicarbonateEnterovirus
The invention discloses a composition for treating fowl enterovirus syndrome and a preparation method thereof, which aim at providing a composition with a good curative effect on integrally treating the fowl enterovirus syndrome and the preparation method thereof. The composition consists of the following ingredients in percentage by weight: 5 to 20 percent of sulfaquinoxaline sodium, 5 to 15 percent of ciprofloxacin hydrochloride, 1 to 4 percent of trimethoprim lactate, 1 to 5 percent of vitamin K3, 5 to 20 percent of sodium bicarbonate and the balance anhydrous dextrose. The adopted sulfaquinoxaline sodium has the strong effects on giant eimeria, eimeria brunette, eimeria acervulina and the like, and the synergia effect of the trimethoprim lactate is used as the auxiliary, so the eimeria resistance capability is greatly enhanced. The ciprofloxacin hydrochloride has the strong force on the bacterial infection in the enterovirus syndrome, and the killing effect is realized. The two effects are combined, and the pathogen causing the enterovirus syndrome can be targeted to be killed. The vitamin K3 can be used for controlling the intestinal tract bleeding caused by the enterovirus syndrome and reducing the side effect caused by the intestinal tract bleeding. The sodium bicarbonate can be used for reducing the kidney injury to the fowl caused by the sulfaquinoxaline sodium.
Owner:TIANJIN SHENGJI GRP CO LTD
Traditional Chinese medicine immune stimulant for preventing Eimeria tenella infections
ActiveCN103495091BDelay drug resistanceNo side effectsAnimal feeding stuffAntiparasitic agentsIMMUNE STIMULANTSSide effect
The invention provides a traditional Chinese medicine immune stimulant for preventing Eimeria tenella. The active ingredient of the traditional Chinese medicine immune stimulant comprises caulis spatholobi, sweet wormwood, rubia cordifolia, humifuse euphorbia herb, honeysuckle, belvedere fruit, garlic, java brucea fruit, the root of Chinese pulsatilla, Chinese dates, lalang grass rhizome, lantana camara, herba taxilli, sargentg loryvine, urena lobata, peach juvenile branchlet, hypericum ascyron, gentiana macrophylla, radix stemonae, semen pharbitidis, cortex ailanthi, hempleaf negundo chastetree leaf, scutellaria baicalensis and sophora flavescens. The traditional Chinese medicine immune stimulant is low in drug resistance and few in drug residue, does not have a toxic and side effect, and has a good prevention effect in the aspect of preventing an Eimeria tenella disease.
Owner:CHENGDU CENTURY INVESTMENT
Pure traditional Chinese medicine composition for resisting eimeria tenella
InactiveCN106344883AGood prevention effectGood treatment effectPharmaceutical non-active ingredientsAntiparasitic agentsCoccidiosisMedicine
The invention relates to a pure traditional Chinese medicine composition for resisting eimeria tenella. The pure traditional Chinese medicine composition for resisting eimeria tenella comprises the following bulk pharmaceutical chemicals: sweet wormwood herb, herba polygoni avicularis, herba agrimoniae, the root of Chinese pulsatilla, rhizoma curcumae longae and cortex cinnamomi. The invention also relates to a preparation method of a traditional Chinese medicine composition granule for resisting eimeria tenella. The invention develops the traditional Chinese medicine composition for resisting eimeria tenella aiming at the disease characteristics of morbidity of chickens according to a Chinese veterinary medicine theory and the clinical characteristics of a coccidiosis; the traditional Chinese medicine composition adopts clearing away heat and toxic materials, removing dampness and destroying parasites, and checking dysentery and stopping bleeding as principles of formulating prescription for prescription formulating; multiple set prescriptions are subjected to therapeutic experiments for resisting eimeria tenella and the traditional Chinese medicine composition is obtained through screening.
Owner:SHANDONG ENKANG PHARMA +1
A degenerate primer RT-PCR detection method for Eimeria virus
InactiveCN103898237BEfficient detectionAccurate identificationMicrobiological testing/measurementCoccidiosisPcr method
The invention discloses a degenerate primer RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method for eimeria viruses. A pair of brand-new degenerate primers is provided, and specific degenerate primer RT-PCR amplification is performed on the eimeria viruses by using reverse transcription and touchdown PCR techniques. The degenerate primer RT-PCR detection method is capable of effectively detecting the existence of an eimeria tenella virus and an eimeria stiedae virus, and good in specificity (an electrophoretic band is single). The degenerate primer RT-PCR detection method is used for detecting the eimeria viruses for the first time, and is capable of quickly and accurately identifying whether a coccidium sample to be detected contains a virus-carrying strain or not.
Owner:JILIN UNIV
Method for improving chicken eimeria oocysts sporulation efficiency and application thereof
InactiveCN108102919AOvercome efficiencyOvercoming demandsProtozoaMicroorganism based processesLiquid heightOxygen
The invention relates to a novel method for improving chicken eimeria oocysts sporulation efficiency, in particular to a method for improving the chicken eimeria oocysts sporulation efficiency throughcontrolling the oocyst concentration, the liquid height and the oxygen concentration. The method has the advantages that the chicken eimeria oocysts sporulation efficiency can be greatly improved; the labor and material investment is reduced; the production efficiency is improved.
Owner:TIANJIN HLINTE BIOTECH CO LTD
A kind of traditional Chinese medicine composition against Eimeria tenella
ActiveCN105031245BGood prevention effectGood treatment effectDigestive systemAntiparasitic agentsCoccidiosisPolygonum aviculare
The invention relates to a traditional Chinese medicine composition for resisting eimeria tenella, which comprises the following raw medicines: kuh-seng, dichroa root, polygonum aviculare, chinaberry bark, pulsatilla chinensis, sealwort and white peony root. According to the Chinese veterinary medicine theory, the clinical characters of coccidiosis and the characteristics of chicken diseases, the invention develops a traditional Chinese medicine composition for resisting eimeria tenella. The traditional Chinese medicine composition has the prescription principles of mainly clearing away heat and drying dampness as well as cooling the blood to stop bleeding and reinforcing deficiency in an auxiliary way, is prepared by screening, and has an obvious curative effect on chicken coccidiosis through actual use.
Owner:SHANDONG ENKANG PHARMA
Method for preparing chicken coccidiosis high immunity yolk antibody
InactiveCN101186647BChange the concept of prevention and treatmentAvoid residueEgg immunoglobulinsYolkCoccidiosis
The invention relates to a preparation method of chicken coccidia hyperimmune egg yolk antigen, which comprises (1) extracting and purifying spore reproductive stage antigen, merozoite reproductive stage antigen, and gamete reproductive stage antigen at each growth stage of chicken coccidian, (2), mixing the three antigens in a certain ratio, selecting suitable immunity adjuvant to be mixed with the antigens to prepare a mixture antigen, (3), selecting the laying hen without immunity, injecting the mixture antigens prepared by different immunity adjuvant into the muscles of thorax of the immunity chicken, (4), collecting the eggs of the immunity chickens, separating yolk, separating and purifying yolk antibody via water dilution, testing the drug effect of the antibody. The protective rates on E. tenella coccidiosis and E. necatrix coccidiosis respectively reach 100% and 96.7% by clinical test, which anti-coccidia indexes are 184.8 and 165.4. The successful industrialization of the inventive product provides a new route to prevent coccidiosis, and provides the base for resolving drug residue or the like.
Owner:CHENGDU QIANKUN VETERINARY PHARMA
A chicken Eimeria tenella micronematode-2 mutant etmic2-2119
InactiveCN106046136BGood immune protectionDepsipeptidesAntiparasitic agentsSurface displayADAMTS Proteins
The invention relates to the field of gene engineering and provides an eimeria tenella microneme protein-2 mutant EtMIC2-2119 of chickens. The mutant is obtained as follows: in-vitro orthogenesis is performed with EtMIC2 (GenBank:KC333870.1) of wild eimeria tenella SD-01 as basic protein, a gene mutation library is established, yeast is used for surface display of a protein mutant library, altered protein with immunogenicity and immune protection remarkably improved is screened by means of sorting by a flow cytometry, animal immune protection effect evaluation and the like, and the mutant is obtained finally. The mutant altered protein has 11 amino acid mutation sites; the immune protection effect ACI of altered EtMIC2 is improved by about 25.06% while compared with that of natural EtMIC2, and the mutant can serve as a vaccine to be applied in the field of resistance to infection by eimeria tenella of the chickens.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
A chicken Eimeria tenella micronematode-2 mutant etmic2-1130
The invention relates to the field of gene engineering and provides an eimeria tenella microneme protein-2 mutant EtMIC2-1130 of chickens. The mutant is obtained as follows: in-vitro orthogenesis is performed with EtMIC2 (GenBank:KC333870.1) of wild eimeria tenella SD-01 as basic protein, a gene mutation library is established, yeast is used for surface display of a protein mutant library, altered protein with immunogenicity and immune protection remarkably improved is screened by means of sorting by a flow cytometry, animal immune protection effect evaluation and the like, and the mutant is obtained finally. The mutant altered protein has six amino acid mutation sites; the immune protection effect ACI of altered EtMIC2 is improved by about 21.94% while compared with that of natural EtMIC2, and the mutant can serve as a vaccine to be applied in the field of resistance to infection by eimeria tenella of the chickens.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Traditional Chinese medicine composition for preventing and treating chicken coccidiosis as well as preparation method, use method and application of traditional Chinese medicine composition
InactiveCN108175834AHas the effect of repelling insectsReduce loose stoolsAntiparasitic agentsPlant ingredientsCoccidiosisCurative effect
The invention relates to a traditional Chinese medicine composition for preventing and treating chicken coccidiosis as well as a preparation method, a use method and application of the traditional Chinese medicine composition and belongs to the technical field of veterinary traditional Chinese medicines. The traditional Chinese medicine composition is composed of the following components in partsby mass: 30-50 parts of herba verbenae, 15-25 parts of cortex phellodendri, 15-30 parts of gardenia jasminoides ellis, 10-25 parts of rhizoma imperatae and 5-15 parts of rhizoma atractylodis Macrocephalae. The traditional Chinese medicine composition is scientifically formulated according to a basic principle of compatibility in traditional Chinese medicine, so that the finally prepared traditional Chinese medicine composition has good curative effect on damp-heat syndrome, dysfunction in spleen transportation and transformation and other clinical symptoms caused by E. tenella, and does not affect later product safety.
Owner:SOUTHWEST UNIVERSITY
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