32 results about "Eimeria gilruthi" patented technology

In one aspect, the present invention is directed to a method for preventing or treating necrotic enteritis by administering a hyperimmunized egg product obtained from an egg-producing animal to an avian. The hyperimmunized egg product may contain an antibody specific to an antigen selected from the group consisting of Clostridium perfringens α-toxin, Clostridium perfringens elongation factor Tu (EF-Tu), Clostridium perfringens necrotic enteritis B-like (NetB) toxin, Clostridium perfringens Pyruvate: Ferredoxin oxidoreductase (PFO), and Eimeria tenella elongation factor 1-alpha.

The invention relates to a traditional Chinese medicine composition for prevention and treatment of coccidiosis of poultry. The traditional Chinese medicine composition is prepared from kudzuvine roots, Chinese pulsatilla roots, sophora flavescens, cyrtomium rhizome, herba agrimoniae, twigs and leaves of antifebrile dichroa, coptis chinensis and pomegranate barks. The traditional Chinese medicine composition can be oral liquid and prepared through the steps that traditional Chinese medicine is smashed to 30 meshes to 50 meshes, weighed according to the formula amount, and mixed in a three-dimensional mode to obtain traditional Chinese medicine powder; the mixed traditional Chinese medicine powder and water are put into an extraction pot and the mixed traditional Chinese medicine powder is soaked for 5 hours to 20 hours; hydrolysate is heated to 40 DEG C to 100 DEG C and extracted for 6 hours to 10 hours; decoction dregs are filtered out, a bottle is directly filled with obtained liquid, and then a finished product is obtained. The traditional Chinese medicine composition can effectively prevent and treat cecum coccidiosis and small intestine coccidiosis of poultry, the rate of killing soft eimeria coccidium and poison eimeria coccidium can reach more than 95%, and the composition also has an obvious and rapid treatment effect on secondary escherichia coli infection caused by coccidiosis. The traditional Chinese medicine composition has the functions of clearing heat and removing toxicity, eliminating dampness, killing coccidium and promoting spleen yang and has the anti-diarrhea effect.

The invention provides a feed for treating Eimeria tenella infection, and its raw materials include wheat, soybean meal, bone meal, rapeseed meal, calcium dihydrogen phosphate, vitamin E, vitamin K, pantothenic acid, lysine, and methionine , threonine and the above-mentioned traditional Chinese medicine preparation for treating Eimeria tenella. The traditional Chinese medicine preparation contained in the feed has low drug resistance, few drug residues, no toxic and side effects, and has good curative effect in treating Eimeria tenella.

The invention discloses a degenerate primer RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method for eimeria viruses. A pair of brand-new degenerate primers is provided, and specific degenerate primer RT-PCR amplification is performed on the eimeria viruses by using reverse transcription and touchdown PCR techniques. The degenerate primer RT-PCR detection method is capable of effectively detecting the existence of an eimeria tenella virus and an eimeria stiedae virus, and good in specificity (an electrophoretic band is single). The degenerate primer RT-PCR detection method is used for detecting the eimeria viruses for the first time, and is capable of quickly and accurately identifying whether a coccidium sample to be detected contains a virus-carrying strain or not.

The invention relates to a traditional Chinese medicine composition for resisting eimeria tenella, which comprises the following raw medicines: kuh-seng, dichroa root, polygonum aviculare, chinaberry bark, pulsatilla chinensis, sealwort and white peony root. According to the Chinese veterinary medicine theory, the clinical characters of coccidiosis and the characteristics of chicken diseases, the invention develops a traditional Chinese medicine composition for resisting eimeria tenella. The traditional Chinese medicine composition has the prescription principles of mainly clearing away heat and drying dampness as well as cooling the blood to stop bleeding and reinforcing deficiency in an auxiliary way, is prepared by screening, and has an obvious curative effect on chicken coccidiosis through actual use.

The invention relates to a preparation method of chicken coccidia hyperimmune egg yolk antigen, which comprises (1) extracting and purifying spore reproductive stage antigen, merozoite reproductive stage antigen, and gamete reproductive stage antigen at each growth stage of chicken coccidian, (2), mixing the three antigens in a certain ratio, selecting suitable immunity adjuvant to be mixed with the antigens to prepare a mixture antigen, (3), selecting the laying hen without immunity, injecting the mixture antigens prepared by different immunity adjuvant into the muscles of thorax of the immunity chicken, (4), collecting the eggs of the immunity chickens, separating yolk, separating and purifying yolk antibody via water dilution, testing the drug effect of the antibody. The protective rates on E. tenella coccidiosis and E. necatrix coccidiosis respectively reach 100% and 96.7% by clinical test, which anti-coccidia indexes are 184.8 and 165.4. The successful industrialization of the inventive product provides a new route to prevent coccidiosis, and provides the base for resolving drug residue or the like.

The invention relates to the field of gene engineering and provides an eimeria tenella microneme protein-2 mutant EtMIC2-2119 of chickens. The mutant is obtained as follows: in-vitro orthogenesis is performed with EtMIC2 (GenBank:KC333870.1) of wild eimeria tenella SD-01 as basic protein, a gene mutation library is established, yeast is used for surface display of a protein mutant library, altered protein with immunogenicity and immune protection remarkably improved is screened by means of sorting by a flow cytometry, animal immune protection effect evaluation and the like, and the mutant is obtained finally. The mutant altered protein has 11 amino acid mutation sites; the immune protection effect ACI of altered EtMIC2 is improved by about 25.06% while compared with that of natural EtMIC2, and the mutant can serve as a vaccine to be applied in the field of resistance to infection by eimeria tenella of the chickens.