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Preparation method of coccidiosis in chicken obtained by ultraviolet ray and cobalt60gamma-ray irradiation as well as precocity breeding

A technology of ray irradiation and chicken coccidiosis, which is applied in the field of preparation of chicken coccidiosis vaccine, can solve the problems of difficulty in weakening coccidia oocysts, reinvigoration, and distant targets for practical application, so as to shorten the time used and improve efficiency effect

Inactive Publication Date: 2010-02-10
QINGDAO SHENGNUOWEITE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this virulent strain vaccine has not been widely used in commercial broilers for more than 40 years. The main reason is that it has two major defects: one is that each coccidia strain present in the vaccine is completely pathogenic; Due to the inconsistency in the amount of oocysts ingested by chickens, some chickens are too lightly infected to develop immunity, and some chickens ingest too much oocysts and cause disease
However, chicken embryo subculture has four disadvantages: (1) The attenuation performance is relatively unstable, and the virulence may become stronger when passaged in chickens; (2) A large number of commercial attenuated coccidia can be obtained through chicken embryo attenuation Oocysts are more difficult; (3) chicken embryos are weakened, and the immunogenicity of the parasites will gradually decrease or lose as the number of generations increases; (4) the main limitation lies in the most popular giant, pile-type and Although Eimeria precocci can develop in chicken embryos, the growth of oocysts is poor, so the production of full-price attenuated vaccines through chicken embryo passage is limited
Although the preparation technology of genetically engineered coccidian vaccines has made progress in recent years, it is still far away from the goal of practical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0018] Specific embodiment one: collect chicken coccidia sample in remote mountainous area free-range chicken, utilize single oocyst separation technique to obtain four kinds of Eimeria (Eimeria tenella, Eimeria hemiformis, Eimeria giantis) Purified oocysts of coccidia and Eimeria spp. were inoculated to chicks grown in a coccidia-free environment, and the offspring oocysts were collected. The progeny oocyst suspension of four kinds of Eimeria coccidia was poured into a plate with a depth of 2mm, and placed under 254nm ultraviolet light for 30-60s.

[0019] Put the treated oocysts into a petri dish with 10 times the volume of 2.5% potassium dichromate solution, the depth of the solution is about 5mm, put it in a 28°C incubator, and cultivate it for 48 hours.

[0020] With the above-mentioned coccidia oocysts treated by ultraviolet light as parents, according to the principle of chicken coccidia precocious selection and weakening, Eimeria tenabilis, Eimeria pileosa, Eimeria gig...

specific Embodiment approach 2

[0021] Specific embodiment two: adopt the method for specific embodiment one to collect offspring oocysts; Place the offspring oocyst suspension of four kinds of Eimeria cobalt 60 gamma-rays to irradiate, the suspension is deep 2mm, and the irradiation dose is 1-2KGy; put the treated oocysts into a petri dish with 10 times the volume of 2.5% potassium dichromate solution, the depth of the solution is about 5mm, put it in a 28°C incubator, and incubate for 48 hours.

[0022] Using the above-mentioned coccidia oocysts treated with cobalt 60γ-rays as parents, according to the principle of precoccidia selection and weakening of chicken coccidia, Eimeria tenabilis, Eimeria hemiformis, and Eimeria gigas were selected. Coccidia, precocious strains of poisonous Eimeria; the sporulated oocysts of four kinds of Eimeria gallinarum obtained by precocious selection and breeding are mixed in proportion to obtain chicken coccidiosis tetravalent (Eimeria tenella , Eimeria acuminata, Eimeria m...

specific Embodiment approach 3

[0023] Specific embodiment three: adopt the method for specific embodiment one to collect progeny oocysts; Place the progeny oocyst suspension of four kinds of Eimeria cobalt 60 gamma-rays to irradiate, the suspension is 2mm deep, and the irradiation dose is 1-2KGy; and then place the four Eimeria progeny oocyst suspensions under 254nm ultraviolet light for 30-60s.

[0024] Put the treated oocysts into a petri dish with 10 times the volume of 2.5% potassium dichromate solution, the depth of the solution is about 5mm, put it in a 28°C incubator, and cultivate it for 48 hours.

[0025] With the coccidia oocysts after the above-mentioned treatment as parents, according to the principle of chicken coccidia premature selection and weakening, Eimeria tenacia, Eimeria cumulus, Eimeria giant, and poisonous Eimeria were selected The precoccidiosis strain of Eimeria; the sporulated oocysts of four kinds of Eimeria gallinarum obtained by prematurity selection and breeding are mixed in pr...

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Abstract

The invention discloses a preparation method of coccidiosis in chicken obtained by ultraviolet ray and cobalt60gamma-ray irradiation as well as precocity breeding. The preparation method comprises: sampling; propagating oocysts; irradiating the offspring oocysts with ultraviolet rays and cobalt60gamma-rays; determining the dosage of ultraviolet ray and cobalt60gamma-ray irradiation; obtaining seven types of Eimeria (Eimeria tenella, Eimeria acervulina, Eimeria maxima, Eimeria necatrix, Eimeria brunette, Eimeria mitis, and precocious Eimeria) precocious strain oocysts; taking the Eimeria oocysts processed by ultraviolet ray and cobalt60gamma-ray irradiation in steps 3 as the parent to carry out coccidiosis in chicken precocity breeding; and blending. The method can greatly shorten the timefor precocity breeding and improve breeding efficiency, thus obtaining plentiful commercialized precocity attenuated Eimeria strains in a short time.

Description

Technical field: [0001] The invention relates to a preparation method of a veterinary vaccine, in particular to a preparation method of chicken coccidiosis vaccine obtained by irradiating ultraviolet rays and cobalt 60 gamma-rays and precocious selection. Background technique: [0002] Chicken coccidiosis is a disease characterized by enteritis and bloody dysentery caused by one or more species of coccidiosis of the genus Eimeria parasitizing in chicken intestinal epithelial cells. Young chickens within 15-50 days are the most susceptible to infection, with a morbidity rate of up to 100%, a fatality rate of 20%-30%, and severe cases up to 80%. It is one of the important diseases that endanger the chicken industry. [0003] At present, there are mainly two methods for the prevention and treatment of the disease, drug control and vaccine control, wherein drug control is achieved by adding a preventive or therapeutic amount of anticoccidiostats to the feed or drinking water. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/012A61P33/02
Inventor 王江青
Owner QINGDAO SHENGNUOWEITE BIOTECH
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