Method for preparing chicken coccidiosis high immunity yolk antibody

A technology of egg yolk antibody and chicken coccidia is applied in the field of preparation of chicken coccidia high immunity egg yolk antibody, and can solve problems such as drug residues and the like

Inactive Publication Date: 2010-04-21
CHENGDU QIANKUN VETERINARY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After injection or oral administration, the antibody can effectively prevent and treat chicken coccidiosis, reduce clinical drug resistance in chicken flocks, and solve problems such as drug residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Using the spore reproductive stage antigen, schizogenetic stage antigen and gamete reproductive stage antigen of each developmental stage of Eimeria tenella strain, the mixed antigen for immunization is prepared according to the above steps. 50 non-immunized laying hens were selected, and the prepared mixed immunization antigen was injected into the breast muscle of each hen for immunization once every 7 days, and the immunization was injected continuously for 3 times, and the immunization dose for each injection was 1ml. Eggs were collected on day 7 after the third immunization. Separate the egg white to obtain the egg yolk, dilute the egg yolk liquid with 10 times of water, adjust the pH value to 5.2, let stand at 4°C for more than 6 hours, centrifuge to remove particles, concentrate with sulfate precipitation, and purify into the required high-immune egg yolk antibody after ultrafiltration , and detected by agar diffusion test, the titer is above 1:64 as qualified. ...

Embodiment 2

[0032] Using the spore reproductive stage antigens, merozoite reproductive stage antigens and gamete reproductive stage antigens of each developmental stage of Eimeria chicken poisoning strain, the mixed antigens for immunization are prepared according to the above steps.

[0033] 50 non-immunized laying hens were selected, and the prepared immunization mixed antigen was injected into the breast muscle of each chicken for immunization once every 7 days, and the immunization was injected continuously for 3 times, and the immunization dose for each injection was 1ml. Eggs were collected on day 7 after the third immunization. Separate the egg white to obtain the egg yolk, dilute the egg yolk liquid with 10 times of water, adjust the pH value to 5.2, let stand at 4°C for more than 6 hours, centrifuge to remove particles, concentrate with sulfate precipitation, and purify into the required high-free egg yolk after ultrafiltration Antibody, and detected by agar diffusion test, the t...

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PUM

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Abstract

The invention relates to a preparation method of chicken coccidia hyperimmune egg yolk antigen, which comprises (1) extracting and purifying spore reproductive stage antigen, merozoite reproductive stage antigen, and gamete reproductive stage antigen at each growth stage of chicken coccidian, (2), mixing the three antigens in a certain ratio, selecting suitable immunity adjuvant to be mixed with the antigens to prepare a mixture antigen, (3), selecting the laying hen without immunity, injecting the mixture antigens prepared by different immunity adjuvant into the muscles of thorax of the immunity chicken, (4), collecting the eggs of the immunity chickens, separating yolk, separating and purifying yolk antibody via water dilution, testing the drug effect of the antibody. The protective rates on E. tenella coccidiosis and E. necatrix coccidiosis respectively reach 100% and 96.7% by clinical test, which anti-coccidia indexes are 184.8 and 165.4. The successful industrialization of the inventive product provides a new route to prevent coccidiosis, and provides the base for resolving drug residue or the like.

Description

technical field [0001] The invention discloses a method for preparing chicken coccidian hyperimmune yolk antibody, and belongs to the technical field of biological preparation and poultry disease prevention and control. Background technique [0002] Chicken coccidiosis is one of the major diseases that endanger the chicken industry. The annual loss caused by chicken coccidiosis in the world reaches 8 billion US dollars. At present, the clinical prevention and treatment of this disease is mainly through chemical drugs. Due to unreasonable The drug regimen caused the ubiquity of drug resistance and serious drug residues, which brought great difficulties to the prevention and treatment of the disease, and caused problems such as food safety. [0003] Avian egg yolk antibody (egg yolk immunoglobulin, IgY) is a globulin produced by poultry B lymphocytes stimulated by antigenic substances and deposited in egg yolk, which can specifically react with antigen. exact advantages. IgY...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/02
Inventor 房春林王万平
Owner CHENGDU QIANKUN VETERINARY PHARMA
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