Resistance index estimation method for selective breeding of chicken eimeria tenella resistant individuals

A technology for Eimeria coccidiosis and selective breeding, applied in applications, animal taming devices, other equipment, etc., can solve the problems of high risk of attacking the virus, difficulty in implementation and promotion, and achieve significant and safe results

Inactive Publication Date: 2015-01-07
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Corresponding resistance indicators can be determined through large doses of chicken coccidiosis sporulated soft sacs

Method used

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  • Resistance index estimation method for selective breeding of chicken eimeria tenella resistant individuals
  • Resistance index estimation method for selective breeding of chicken eimeria tenella resistant individuals

Examples

Experimental program
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Effect test

Embodiment 1

[0010] Twenty-five Liangfeng chickens and Lingnan yellow chickens were immunized with the canonical coccidiosis quadrivalent live vaccine at the age of 9 days by the above method, and NO (μmol / mL) and CAT (ng / mL) in plasma at the age of 17 days were determined by ELISA , SOD(ng / mL), IL-17(ng / L), GSH-PX(U / mL), MDA(μmol / mL), IL-2(ng / L), IL-16(ng / L) , IFN-γ (μmol / mL) and carotene (ng / mL) concentrations. At the age of 39 days, 25,000 sporulated oocysts of E.tenella were challenged. ELISA method was used to measure NO (μmol / mL), CAT (ng / mL), SOD (ng / mL), IL- 17(ng / L), GSH-PX(U / mL), MDA(μmol / mL), IL-2(ng / L), IL-16(ng / L), IFN-γ(μmol / mL) and β-C (ng / mL) concentration. The correlation coefficients of each index after immunization and challenge were calculated, and regression analysis was carried out. The results are shown in Table 1 below. The determination coefficients of CAT, SOD, GSH-Px, MDA, and IFN-γ are greater than 0.81, so the relationship between these indicators after immu...

Embodiment 2

[0017] 25 Liangfeng chickens and 25 Lingnan yellow chickens were immunized with 500 activated Eimeria tenella at the age of 9 days by the above method, and NO (μmol / mL) and CAT (ng / mL), SOD(ng / mL), IL-17(ng / L), GSH-PX(U / mL), MDA(μmol / mL), IL-2(ng / L), IL-16(ng / L), IFN-γ (μmol / mL) and carotene (ng / mL) concentrations. At the age of 39 days, 25,000 sporulated oocysts of E.tenella were challenged. ELISA method was used to measure NO (μmol / mL), CAT (ng / mL), SOD (ng / mL), IL- 17(ng / L), GSH-PX(U / mL), MDA(μmol / mL), IL-2(ng / L), IL-16(ng / L), IFN-γ(μmol / mL) and β-C (ng / mL) concentration. The correlation coefficients of each index after immunization and challenge were calculated, and regression analysis was carried out. The results are shown in Table 3 below. The determination coefficients of CAT, SOD, GSH-Px, MDA, and IFN-γ are greater than 0.81, so the relationship between these indicators after immunization and after challenge is highly correlated. The established regression equati...

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Abstract

The invention relates to the field of poultry breeding and specifically relates to a method for accurately estimating each index concentration after challenge of chicken eimeria tenella according to resistance indexes measured after immunization with a chicken coccidiosis vaccine. The method comprises the steps of immunizing samples to be screened with the chicken coccidiosis vaccine and then measuring five resistance indexes in the blood plasma, namely CAT, SOD, GSH-Px, MDA and IFN-gamma, and then accurately estimating the corresponding concentration of each index after the challenge (infection) of the chicken eimeria tenella by use of a pre-established regression equation of each resistance index according to the five resistance indexes measured after immunization with the chicken coccidiosis vaccine so as to select chicken eimeria tenella resistant individuals by use of the five resistance indexes under the condition of non-challenge (infection), wherein the regression equation of the resistance index refers to the quantitative relation of the resistance index after immunization with the chicken coccidiosis vaccine and challenge (infection). The method is capable of realizing conventional selective breeding of the chicken coccidiosis, and is safe and significant.

Description

technical field [0001] The invention relates to the field of poultry breeding, in particular to a method for estimating the resistance index after chicken coccidiosis vaccine immunization. Background technique [0002] Chicken coccidiosis is an epidemic acute parasitic disease that seriously harms breeding enterprises. The incidence rate is highest in chickens aged 10 to 60 days. The main characteristics are chicken emaciation, loss of appetite, anemia and bloody dysentery. Growth is stunted, weight gain and egg production are affected. The economic loss caused by chicken coccidiosis in the world is as high as 3 billion U.S. dollars every year, and the annual cost of medicines used to prevent and control chicken coccidiosis in my country has reached hundreds of millions of yuan, accounting for nearly one-third of the total cost of chicken disease control . For a long time, coccidiosis in chickens has been mainly controlled by drugs, but if a drug is used for a long time, it...

Claims

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Application Information

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IPC IPC(8): A01K67/02A01K15/00
CPCA01K29/00A01K67/02
Inventor 戴国俊杨建生林雨鑫张菁菁张根喜王金玉谢恺舟孙明明顾玉萍王永娟
Owner YANGZHOU UNIV
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