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SdLDL-C (small and dense LDL cholesterol) detection kit

A low-density lipoprotein and detection kit technology, which is applied in measuring devices, microbial determination/inspection, instruments, etc., can solve the problems of waste of medical resources, short validity period, poor stability of bottle opening, etc., and achieve batch-to-batch precision Good, high accuracy, high sensitivity effect

Active Publication Date: 2018-08-21
天津中成佳益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new type of detective tool for measuring very tiny particles called LDL (low-densitieslipoproteins) used by medical professionals during tests on blood samples was developed compared to previous tools that had issues such as being too large or bulky due to their use of two different agents separately containing these substances. However this new method provides better results than older methods while also improving upon existing ones' performance over time.

Problems solved by technology

This patented technical problem addressed by this patents relates to improving methods or devices for detecting low- densities lipoprostreatosis (low-densitility lipemia) among patients who require therapy due to their condition. Current testing techniques involve measuring total plasminogen activator protein concentration (TAP) but these tests only provide limited data on specific types of lipoplasma present at different stages during developmental process.

Method used

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  • SdLDL-C (small and dense LDL cholesterol) detection kit
  • SdLDL-C (small and dense LDL cholesterol) detection kit
  • SdLDL-C (small and dense LDL cholesterol) detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The specific components of the liquid double reagent of a small and dense low-density lipoprotein cholesterol detection kit are as follows:

[0035]

[0036]

[0037]

[0038] 1) Determination of accuracy.

[0039] Repeat the measurement 3 times with the same quality control product (LEVEL 1 and LEVEL 2), take the average value, and the relative deviation from the target value of the quality control product should be controlled within 10%. The test results of the target value of the quality control product are shown in Table 1; The contrast reagent of stabilizing agent and the test result of detection kit reagent of the present invention 2 months are shown in Table 2; table 3.

[0040] Table 1 Test results of quality control target value

[0041] Quality Control

Target value (mmol / L)

Range (mmol / L)

LEVEL1

0.8

0.72-0.88

LEVEL2

2.01

1.809-2.211

[0042] Table 2 Accuracy test results after opening the bottle for 2...

Embodiment 2

[0055] The specific components of the liquid double reagent of a small and dense low-density lipoprotein cholesterol detection kit are as follows:

[0056]

[0057]

[0058]

[0059] Identical with the method of embodiment 1, above-mentioned reagent is done accuracy, precision and linear range test, and the result shows, the difference between the data of accuracy, precision and linear range and the reagent described in example 1 is within the normal range of error Inside.

Embodiment 3

[0061] The specific components of the liquid double reagent of a small and dense low-density lipoprotein cholesterol detection kit are as follows:

[0062]

[0063]

[0064] Table 5 shows the results of the blank absorbance detection test done with the above reagents.

[0065] Table 5 blank absorbance detection results

[0066]

[0067] Table 5 blank absorbance detection results show that the blank absorbance of the contrast reagent without adding dithiothreitol increases significantly when the bottle is opened for 1 month, and the blank absorbance of the reagent kit described in this example is ≤ 1 month after opening the bottle. 0.20, a certain proportion of dithiothreitol in the kit reagent described in the present embodiment has played the effect of protecting enzyme, and dithiothreitol can protect 4-aminoantipyrin in the reagent from being oxidized as a reducing agent and cause blank increase. Big.

[0068] As can be seen from the above examples, a small and de...

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Abstract

The invention provides an SdLDL-C (small and dense LDL cholesterol) detection kit. The kit comprises liquid dual reagents and comprises a reagent R1 and a reagent R2, wherein the reagent R2 comprisescomponents as follows: a buffer solution with the concentration of 0.1-1.0 mol/L, peroxidase with the concentration of 100-200 U/L, 4-amino-antipyrine with the concentration of 1.0-2.5 mol/L, triton with the concentration of 40-60.0 mmol/L and a stabilizer with the concentration of 2.5-5 ml/L. According to the SdLDL-C detection kit, both the reagent R1 and the reagent R2 contain the stabilizer, the decapping stability and the expiry date stability of the SdLDL-C detection kit can be notably improved, and the reagents have high accuracy, high sensitivity and good between-run precision and can meet the clinical examination demands.

Description

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Claims

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Application Information

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Owner 天津中成佳益生物科技有限公司
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