Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

HIV-1 broad-spectrum neutralizing antibody and use thereof

An antibody, human monoclonal antibody technology, applied in the direction of antibodies, antiviral agents, chemical instruments and methods, etc., can solve the problems of limited neutralization width and large variation of HIV-1 strains

Active Publication Date: 2022-01-28
NANKAI UNIV +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of large variation of HIV-1 strains and limited neutralization width of traditional monoclonal antibodies, and to provide a strain of HIV-1 broad-spectrum neutralizing antibody and its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HIV-1 broad-spectrum neutralizing antibody and use thereof
  • HIV-1 broad-spectrum neutralizing antibody and use thereof
  • HIV-1 broad-spectrum neutralizing antibody and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0079] Example: Broad-spectrum neutralizing antibody F6

[0080] Antibody F6 isolated from HIV-1 infected patients in China

[0081]The inventor isolated a monoclonal antibody from a Chinese HIV-1 infected person and named it F6. Escherichia coli carrying the expression vector (F6H) of the heavy chain gene and the expression vector (F6K) of the light chain gene containing antibody F6 were deposited in China under the preservation numbers CGMCC No.16431 and CGMCC No.16432 respectively China General Microbiological Culture Collection Center (CGMCC) (No. 1 Beichen West Road, Chaoyang District, Beijing). The coding sequence of the heavy chain variable region contained in F6H is SEQ ID NO:1, and the amino acid sequence of the heavy chain variable region encoded by it is SEQ ID NO:2. The coding sequence of the light chain variable region contained in F6K is SEQ ID NO:3, and the amino acid sequence of the light chain variable region encoded by it is SEQ ID NO:4. Table 1 gives the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

HIV-1 broad-spectrum neutralizing antibody and its application. The invention relates to an HIV-1 broad-spectrum neutralizing antibody, comprising a heavy chain variable region and a light chain variable region; the antibody specifically binds to HIV-1 gp120. The present invention also relates to the preparation method and application of the antibody.

Description

technical field [0001] The present invention relates to HIV-1 broadly neutralizing antibodies that specifically bind HIV-1 gp120. The present invention also relates to the preparation method and application of the antibody. Background technique [0002] HIV-1 neutralizing antibodies can effectively prevent HIV-1 from binding and entering human CD4+ T cells. Therefore, as a treatment for HIV-1 infected persons, the field of AIDS research has been devoting itself to the development of potent HIV-1 broad-spectrum neutralizing antibodies. [0003] Monoclonal antibody b12 was first obtained by phage display library technology, but it can only neutralize about 40% of known HIV-1 viruses [1] . Since 2010, thanks to the development of technologies such as single B cell sorting and deep sequencing, a variety of human monoclonal antibodies with broad-spectrum neutralizing activity against HIV-1 have been isolated from HIV-1 infected patients , such as VRC01 targeting the CD4 bindi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10A61K39/42A61P31/18G01N33/577G01N33/569
CPCA61P31/18C07K16/1063C07K2317/76C07K2317/565
Inventor 邵一鸣鞠斌魏民任莉郝彦玲李丹
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com