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HIV-1 broadly neutralizing antibody and use thereof

An antibody and application technology, applied in the field of preparation of the antibody, can solve the problems of limited neutralization width, large variation of HIV-1 strain and the like

Active Publication Date: 2019-01-22
NANKAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of large variation of HIV-1 strains and limited neutralization width of traditional monoclonal antibodies, and to provide a strain of HIV-1 broad-spectrum neutralizing antibody and its application

Method used

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  • HIV-1 broadly neutralizing antibody and use thereof
  • HIV-1 broadly neutralizing antibody and use thereof
  • HIV-1 broadly neutralizing antibody and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0079] Example: Broad-spectrum neutralizing antibody F6

[0080] Antibody F6 isolated from HIV-1 infected patients in China

[0081]The inventor isolated a monoclonal antibody from a Chinese HIV-1 infected person and named it F6. Escherichia coli carrying the expression vector (F6H) of the heavy chain gene and the expression vector (F6K) of the light chain gene containing antibody F6 were deposited in China under the preservation numbers CGMCC No.16431 and CGMCC No.16432 respectively China General Microbiological Culture Collection Center (CGMCC) (No. 1 Beichen West Road, Chaoyang District, Beijing). The coding sequence of the heavy chain variable region contained in F6H is SEQ ID NO:1, and the amino acid sequence of the heavy chain variable region encoded by it is SEQ ID NO:2. The coding sequence of the light chain variable region contained in F6K is SEQ ID NO:3, and the amino acid sequence of the light chain variable region encoded by it is SEQ ID NO:4. Table 1 gives the ...

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Abstract

The present invention relates to a HIV-1 broadly neutralizing antibody and use thereof, and relates to HIV-1 broadly neutralizing antibodyies. The HIV-1 broadly neutralizing antibody comprises a heavychain variable region and a light chain variable region, and can be specifically bonded with HIV-1gp120. The present invention also relates to a preparation method, and the use of the HIV-1 broadly neutralizing antibody.

Description

technical field [0001] The present invention relates to HIV-1 broadly neutralizing antibodies that specifically bind HIV-1 gp120. The present invention also relates to the preparation method and application of the antibody. Background technique [0002] HIV-1 neutralizing antibodies can effectively prevent HIV-1 from binding and entering human CD4+ T cells. Therefore, as a treatment for HIV-1 infected persons, the field of AIDS research has been devoting itself to the development of potent HIV-1 broad-spectrum neutralizing antibodies. [0003] The monoclonal antibody b12 was first obtained by phage display library technology, but it can only neutralize about 40% of known HIV-1 viruses [1] . Since 2010, thanks to the development of technologies such as single B cell sorting and deep sequencing, a variety of human monoclonal antibodies with broad-spectrum neutralizing activity against HIV-1 have been isolated from HIV-1 infected patients , such as VRC01 targeting the CD4 b...

Claims

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Application Information

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IPC IPC(8): C07K16/10A61K39/42A61P31/18G01N33/577G01N33/569
CPCA61P31/18C07K16/1063C07K2317/76C07K2317/565
Inventor 邵一鸣鞠斌魏民任莉郝彦玲李丹
Owner NANKAI UNIV
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