Radix aconiti carmichaeli fingerprint spectrum, establishment method of radix aconiti carmichaeli fingerprint spectrum, and radix aconiti carmichaeli quality detection method

A technique of fingerprinting and establishing methods, which is applied in the field of drug testing, can solve problems such as the single quality evaluation method of aconite, achieve good reference, simplify the analysis process, and achieve good stability

Active Publication Date: 2019-02-01
YAAN THREE NINE PHARMA
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  • Claims
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Problems solved by technology

At present, the quality evaluation method of aconite in the Chinese Pharmacopoeia is only single, which is not enough to systematically and completely indicate the overall quality of aconite and its preparations

Method used

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  • Radix aconiti carmichaeli fingerprint spectrum, establishment method of radix aconiti carmichaeli fingerprint spectrum, and radix aconiti carmichaeli quality detection method
  • Radix aconiti carmichaeli fingerprint spectrum, establishment method of radix aconiti carmichaeli fingerprint spectrum, and radix aconiti carmichaeli quality detection method
  • Radix aconiti carmichaeli fingerprint spectrum, establishment method of radix aconiti carmichaeli fingerprint spectrum, and radix aconiti carmichaeli quality detection method

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Effect test

Embodiment 1

[0077] The present embodiment provides a method for establishing aconite fingerprint method, which mainly includes the following steps:

[0078] Prepare reference substance solution: adopt 50vol% methanol aqueous solution to dissolve benzoyl neoaconitine, benzoyl aconitine, benzoyl hypoaconitine to prepare reference substance solution, so that every 1ml reference substance solution contains Formyl neoaconitine 10 μg, benzoyl aconitine 10 μg and benzoyl hypoaconitine 10 μg;

[0079] Prepare aconite need testing solution; get aconite powder, add 50vol% ethanol aqueous solution, close up, sonicate for 30min, filter, decompress and recover the solvent in the filtrate to dryness, then adopt 50vol% methanol aqueous solution to dissolve and settle to 5ml; aconite powder and The ratio of the aqueous ethanol solution was 0.04 g / ml.

[0080] Accurately draw 10 μl each of the reference substance solution and the test solution, respectively inject into the liquid chromatograph, measure, re...

Embodiment 2

[0087] The present embodiment provides a method for establishing aconite fingerprint method, which mainly includes the following steps:

[0088] Prepare reference substance solution: adopt 48vol% methanol aqueous solution to dissolve benzoyl neoaconitine, benzoyl aconitine, benzoyl hypoaconitine to prepare reference substance solution, so that every 1ml reference substance solution contains Formyl neoaconitine 8 μg, benzoyl aconitine 8 μg and benzoyl aconitine 8 μg;

[0089] Prepare aconite test solution; get aconite powder, add 35vol% ethanol aqueous solution, plug, ultrasonically treat for 40min, filter, decompress and reclaim the solvent in the filtrate to dryness, then adopt 35vol% methanol aqueous solution to dissolve and settle to 4ml; aconite powder and The proportion of ethanol aqueous solution is 0.03g / ml;

[0090] Precisely draw 10 μl each of the reference substance solution and the test solution, respectively, and inject them into the liquid chromatograph for deter...

Embodiment 3

[0097] The present embodiment provides a method for establishing aconite fingerprint method, which mainly includes the following steps:

[0098] Prepare reference substance solution: adopt 52vol% methanol aqueous solution to dissolve benzoyl neoaconitine, benzoyl aconitine, benzoyl hypoaconitine to prepare reference substance solution, make every 1ml reference substance solution contain Formyl neoaconitine 9 μg, benzoyl aconitine 9 μg and benzoyl aconitine 9 μg;

[0099] Prepare aconite need testing solution; get aconite powder, add 45vol% ethanol aqueous solution, close up, ultrasonic treatment 40min, filter, decompress and recover the solvent in the filtrate to dryness, then adopt 45vol% methanol aqueous solution to dissolve and settle to 4ml; Aconite powder and The proportion of ethanol aqueous solution is 0.05g / ml;

[0100] Precisely draw 10 μl each of the reference substance solution and the test solution, respectively, and inject them into the liquid chromatograph for d...

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Abstract

The invention relates to the technical field of drug detection, and particularly relates to a radix aconiti carmichaeli fingerprint spectrum, an establishment method of the radix aconiti carmichaeli fingerprint spectrum, and a radix aconiti carmichaeli quality detection method. The establishment method of the radix aconiti carmichaeli fingerprint spectrum mainly comprises the following steps of preparing a reference solution, and preparing a radix aconiti carmichaeli test solution; and measuring the radix aconiti carmichaeli test solution and the reference solution by high performance liquid chromatography. The method provided by an embodiment of the invention is stable, reliable, simple to operate and good in repeatability. The fingerprint spectrum analysis method parameters provided by the invention can meet relatively high requirements in precision, repeatability, durability and specificity, so that the stability and reliability of the method are proved.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a fingerprint of aconite, a method for establishing the same and a method for detecting the quality of aconite. Background technique [0002] Aconite, the name of traditional Chinese medicine. It is a processed product of the root of Aconitum aconitum of Ranunculaceae, also known as Fupian, Yanfuzi and Fuzi. At present, the quality evaluation method of aconite in the "Chinese Pharmacopoeia" is only single, which is not enough to systematically and completely indicate the overall quality of aconite and its preparations. Contents of the invention [0003] The purpose of the present invention is to provide an aconite fingerprint, its establishment method and aconite quality detection method, in order to accurately analyze and measure the aconite quality, can effectively and reliably predict product quality, and provide a basis for quality evaluation. [0004] A first aspe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686
Inventor 王丽婷董礼蒋晓凤韦婷
Owner YAAN THREE NINE PHARMA
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