A Strain of Acidophilus Thiobacillus and Its Application

A thermophilic thiobacillus and microbial preparation technology, applied in the field of bioengineering, can solve the problems of complex operation, poor leaching effect, and high requirements for equipment and equipment, and achieve the effects of increasing the leaching rate, improving the biological leaching process, and low requirements for equipment and equipment

Active Publication Date: 2020-05-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few reports on the cultivation of resistant strains in the field of bioleaching in the existing research, and the operation is complicated, the requirements for equipment are high, and the leaching effect is not good. Thiobacillus acidophilus, which has been popularized and applied on a large scale, has great application value

Method used

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  • A Strain of Acidophilus Thiobacillus and Its Application
  • A Strain of Acidophilus Thiobacillus and Its Application
  • A Strain of Acidophilus Thiobacillus and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Acquisition and Identification of Acidophilus Thiobacillus CCTCC NO:M 2018727

[0026] 1. Copper-resistant domestication of Thiobacillus acidophilus

[0027] The original strain was activated and inoculated in Starkey complex medium containing 1g / L copper ion concentration. The cultured bacterial strains were continuously transferred to new copper-containing Starkey composite medium for cultivation and domestication, and the copper ion concentration of the copper-containing Starkey composite medium for each transfer was 1g / L higher than that of the last transfer. Until the strain cannot grow under higher copper concentration, the current strain is selected as the domesticated strain with copper resistance. Copper-resistant strains have enhanced tolerance to copper ions. The time to enter the stable period during the acclimatization process of copper ions is 15-30 days, and the acclimatization time at this concentration gradually prolongs as the concentration...

Embodiment 2

[0049] Example 2 The original strain and Thiobacillus acidophilus CCTCC NO: M 2018727 leached copper-poor ore under different copper stress conditions

[0050] The original strain, Thiobacillus acidophilus CCTCC NO:M 2018727 strain, was subjected to leaching experiments under different copper ion concentrations. Figure 4 shown.

[0051] Figure 4 In A, it can be seen that in the control group 1 system, that is, under the leaching condition without copper ion stress, the free bacterial concentration of Thiobacillus acidophilus CCTCC NO:M 2018727 during the leaching process was higher than that of the original strain in the early stage, and the trend of change was basically the same. Thiobacillus acidophilus CCTCC NO:M 2018727 was the highest. The final free bacterial concentration of the original strain reached 4.284×10 8 cells / ml, Acidophilus Thiobacillus CCTCC NO:M 2018727 The final free bacterial concentration reached 5.079×10 8 cells / ml, 18.56% higher than the original...

Embodiment 3

[0059] Example 3 Adding silver ions to promote the leaching of copper-depleted ore under copper stress by domesticated strains

[0060] Add 1g / L copper ion concentration in the leaching environment, transfer to the logarithmic Thiobacillus acidothermophilus CCTCC NO:M2018727, and add different concentrations of silver ions for catalysis. After leaching, it was found that the addition of silver ions promoted the leaching of poor copper ore to a certain extent. During the leaching process, indicators such as Figure 5 shown. The figure shows the changes of pH, Eh, concentration of adsorbed bacteria and free bacteria in the leaching system.

[0061] The change trend of each indicator in the leaching system is generally similar. Among the pH indicators, the 10mg / L and 20mg / L silver ion systems fluctuate less, and the time of upward fluctuation in the middle stage of leaching (14d-28d) is delayed by 4 days for other conditions, 10mg / L , The Eh value in the 20mg / L silver ion syst...

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Abstract

The invention discloses a strain of acidophilus thermophilic thiobacterium and its application, in particular to a kind of acidophilus thermophilic thiobacterium with dual metal resistance and a method for biocatalytically leaching chalcopyrite by using the strain, which belongs to the field of bioengineering . The invention domesticates the original mesophilic acidophilic thiobacterium, continuously improves its copper-resistant performance through gradient domestication, leaches the copper-resistant strain and the original strain under different copper stress concentrations, and verifies the improvement of the leaching level of the domesticated strain under various conditions. The domesticated strains are leached in a copper stress environment, and silver ions are added to the leaching system to promote the interaction between ions, which makes the leaching system active, promotes iron-sulfur metabolism, and reduces the formation of passive film. The environment quickly adapted, and finally the copper ion leaching rate increased to 54.71%. The method is simple and easy to operate, low in cost, and suitable for large-scale popularization and application of similar bioleaching processes.

Description

technical field [0001] The invention relates to an acidophilus thermophilic thiobacterium and its application, in particular to an acidophilus thermophilic thiobacterium with dual metal resistance and a method for biocatalytically leaching chalcopyrite by using the strain, which belongs to the field of bioengineering. Background technique [0002] Acidithiobacillus caldus is an obligate chemoautotrophic sulfur-oxidizing bacterium that uses the Calvin cycle to fix carbon dioxide in the air as a carbon source, and can oxidize inorganic reducing sulfides to obtain the energy and energy necessary for growth. Reducing power can effectively promote the release of metal ions, improve metallurgical efficiency, and play an important role in biometallurgy, coal desulfurization, and treatment of sulfur-containing wastewater. [0003] Bioleaching technology refers to the release of precious and rare metal ions in minerals into the solution in the form of ions through a series of biochem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C22B15/00C22B3/18C12R1/01
CPCC12N1/20C22B3/18C22B15/0065C12N1/205C12R2001/01Y02P10/20
Inventor 冯守帅杨海麟崔亚铨尹宗伟侯少翔
Owner JIANGNAN UNIV
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