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59 results about "Thiobacillus sp." patented technology

Efficient biological desulfurization method for coal

The invention discloses an efficient biological desulfurization method for coal, which is characterized in that coal is crushed through a crusher until the particle size is below 150 meshes; and three bacteria, namely Thiobacillus thioxidans, Thiobacillus caldus and Pseudomonas stutzeri, are adopted. The desulfurization process comprises the following sequential steps: removing organic sulfur in the coal through the Pseudomonas stutzeri; after the primary desulfurization is finished, preparing the Thiobacillus thioxidans and the Thiobacillus caldus into a desulfurization solution according to a certain total bacterial count ratio, and removing inorganic sulfur of the coal; and washing the desulfurized coal with water, filtering, and drying. The total sulfur removal ratio of the desulfurized coal is up to 57.8%; and the method is simple to operate, mild in treatment conditions and low in cost, and effectively retains effective components in the coal, thus being a desulfurization technology having very wide market prospects.
Owner:CENT SOUTH UNIV

Means for sequestration and conversion of COx and NOx, CONOx

InactiveCN101918110AInexpensive and safe to removeBroad appealBioreactor/fermenter combinationsBiological substance pretreatmentsEngineeringSulfide
The instant invention presents means for sequestering COx and NOx; further comprising algae means to convert COx into oxygen (O2), as well as biological means to convert sulfides into elemental sulfur. The instant invention comprises algae, heterotrophs, facultative bacteria and Thiobacillus. The instant invention comprises means of light (photon) transfer. Fiber optics is a means of photon transfer to provide photons to a biological reactor. The instant invention comprises the photon depth adsorption capability of algae in biological reactor means. The instant invention comprises means of energy management so that the instant invention may be used in most any environment, wherein a photon (light) source is available and can comprise a means of photon source generation when a light source is not available.
Owner:理查德·艾伦·哈斯

Thiobacillus acidophilus and method for treating acid mine wastewater and recycling iron resource by adopting same

The invention provides thiobacillus acidophilus. The name of the strain is Thiobacillus ferrooxidans ST-A, the Thiobacillus ferrooxidans ST-A is preserved in the China Center for Type Culture Collection (located in Wuhan University) on May 24, 2015, and is assigned with the accession number of CCTCC NO:M2015333. When the strain is adopted for treating iron-containing acid mine wastewater, divalent iron ions are oxidized in an acceleration manner under low pH, and then trivalent iron ions are generated; under the suitable pH, the strain can catalyze the trivalent iron ions to form schwertmannite (Fe8O8(OH)6(SO4)) sediment, so that the iron resource is effectively recycled, meanwhile, the recycled iron-containing sediment does not contain other valuable metal (copper, zinc and the like), and thus an operable process is provided for the resource recycling of metal in the acid mine wastewater.
Owner:有研资源环境技术研究院(北京)有限公司

Method for the identification and quantification of microorganisms useful in biomining processes

The present invention discloses a method to identify and quantify environmental microorganisms useful in biomining processes. These microorganisms are basically 10, belonging to Bacteria: Acidiphilium sp., Leptospirillum sp., Sulfobacillus sp., Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans; and Archaea: Acidianus sp., Ferroplasma sp., Metallosphaera sp., Sulfolobus sp. and Thermoplasma sp.The method comprises performing a PCR with specific primers designed in our laboratories for different taxons SEQ ID No. 4 to SEQ ID No.: 407. With qPCR results and other data obtained from the analyzed sample, the microorganism concentration of each analyzed taxon present in the sample is calculated using a mathematical formula.
Owner:BIOSIGMA

Functional material for removing nitrate nitrogen in water and preparation and application methods thereof

The invention discloses a functional material for removing nitrate nitrogen in water and preparation and application methods thereof. The functional material is obtained by taking sulfur powder and high-activity iron-manganese sulfide powder as main raw materials and taking biomass fibers and a binder as additives, uniformly mixing the raw materials, adding water for molding, and then curing to finish hydration or carbonation. mixing functional material and limestone or dolomite particles and then fillinginto a biological filter, inoculating thiobacillus and culturing until a biological membrane is mature, and treating the wastewater in an upwelling mode according to the hydraulic retention time of 1-5h so that the removal rate of nitrate nitrogen in the wastewater reaches 80% or above.
Owner:HEFEI UNIV OF TECH

Deodorizing filler and preparation method thereof, deodorizing device, deodorizing method, and application

The invention provides a deodorizing filler and a preparation method thereof, a deodorizing device, a deodorizing method, and application, belonging to the technical field of deodorization. The deodorizing filler includes a main filler body and a microbial agent. Based on the mass percentage of the main filler body, the main filler body includes 35 to 45% of fly ash, 20 to 30% of clay, 10 to 20% of dewatered sludge, 5% to 15% organic minerals and 5% to 15% of a binder. The microbial agent includes Thiobacillus, saccharomycete, Bacillus subtilis, Pseudomonas putida and nitrifying bacteria. Thedeodorizing filler has high deodorizing efficiency and can effectively reduce malodorous substances in gas.
Owner:TIANJIN CARING TECH DEV

Biological desulfurizing technology for transferring sulfur-containing compound into elemental sulfur

Disclosed is the bio-desulphurization technology for conversing the sulfur compounds to the elemental sulfur, which can be used to remove the sulfur compounds in the smoke and waste water. The sulfite and sulfate are produced by dissolving the SO2 in the smoke in water; the sulfate reducing bacteria (SRB) deoxidize the sulfite and sulfate to sulfide under the anaerobic environment and the condition of external carbon source; then the sulfid is conversed to the elemental sulfur by the function of desulfuration microorganism (colorless thiobacilli) under the aerobic condition, and then the sulfur element can be removed from the system after deposition and separation. The invention achieves the recovery of elemental sulfur through the efficient biological reaction, thus effectively solving the pollution problem of SO2, realizing resource recovery of waste, and finally achieving the unification of environmental benefits and economic benefits.
Owner:阮文权 +2

Composite microbial deodorizer and preparation method thereof

The invention discloses a composite microbial deodorizer and a preparation method thereof. According to the invention, thiobacillus ferrooxidans, rhizobium sludge, bacillus amyloliquefaciens, rhodopseudomonas palustris, pseudomonas stutzeri, bacillus coagulans and hansenula polymorpha are selected for use; liquid fermentation is performed respectively, and then compounding is performed in proportion; then linalool is added in proportion and the substances are fully mixed to prepare a composite microbial deodorizer capable of effectively expelling flies and insects, inhibiting growth and propagation of harmful flora and reducing and removing foul gas in the environment. The thiobacillus ferrooxidans (CFU / g) in the product is greater than or equal to 2.95*10<8>; the rhizobium sludge (CFU / g)is greater than or equal to 1.05*10<8>; the bacillus amyloliquefaciens (CFU / g) is greater than or equal to 1.73*10<8>; the rhodopseudomonas palustris (CFU / g) is larger than or equal to 2.55*10<8>; thepseudomonas stutzeri (CFU / g) is larger than or equal to 1.15*10<8>; the bacillus coagulans (CFU / g) is larger than or equal to 2.86*10<8>; the hansenula anomala (CFU / g) is larger than or equal to 2.97*10<8>; and the total amount of organic acids is larger than or equal to 25.8 g / L. The product provided by the invention has an obvious deodorization effect, the NH3 removal rate is greater than or equal to 78.14%, the H2S removal rate is greater than or equal to 71.64%, the odor concentration elimination rate is greater than or equal to 84.95%, and the mosquito and fly expelling rate is greater than or equal to 74.16%.
Owner:巴彦淖尔市云天下电子商务股份有限公司

Clean utilization method for municipal sludge

The invention provides a clean utilization method for municipal sludge. According to the method, the municipal sludge is subjected to high-temperature anaerobic fermentation, then, a thiobacillus thiooxidans enriched bacterial solution is added for culture, and finally, the municipal sludge is dehydrated. By the clean utilization method for the municipal sludge, provided by the invention, biogas can be produced and prepared at an anaerobic digestion stage; after heavy metals are removed through thiobacillus thiooxidans treatment, the sludge can be fully decomposed; meanwhile, for municipal industrial sludge, in the case that the content of the heavy metals is in the range of exceeding the heavy-metal limit value, specified by the national sgricultural standard, by five times, the heavy metal removal effect is good, the removal ratio can reach 90-95%, the treated industrial sludge can fully meet the agricultural requirements of sludge, is made into all types of organic fertilizers, is applied to landscaping, agricultural use and the like and is used for increasing the content of organic matters in soil and improving the structure of the soil; whereas, for municipal domestic sludge, the heavy metal removal effect is more obvious.
Owner:新疆天物生态环保股份有限公司

Thermophilic thiobacillus gene engineering bacterium with mercury resistant characteristic and application thereof

InactiveCN101016521AImproved resistance to mercuryBacteriaProcess efficiency improvementMicroorganismThiobacillus
The invention discloses a preparing method of strain thermophilic thiobacilleae with anti-mercury property with keeping number as CGMCC 1742, which comprises the following steps: (1) constructing anti-mercury plasmid carrier; (2) constructing and attesting anti-mercury genetic engineering bacterium; (3) culturing the seed of A.caldus CGMCC 1742; (4) enlarging culture A.caldus CGMCC 1742 (5) growing in mercury culture medium; testing the anti-mercury property. This product possesses stable anti-mercury property, which can be used widely in biological dipping domain.
Owner:SHANDONG UNIV

Microbial water purifying agent for loach culture water and preparation method of microbial water purifying agent

The invention discloses a microbial water purifying agent for loach culture water and a preparation method of the microbial water purifying agent. The preparation method comprises the following steps of adding 30-50 parts by weight of magnetic silicon balls with surfaces modified with C8 and C18 alkyl chains to 300-500 parts by weight of water; adding 100-150 parts by weight of microbial reagents which include the following components in parts by weight: 60-80 parts of rhodopseudomonas palustris, 60-80 parts of synechococcus bacteria, 10-30 parts of nitrococcus, 20-40 parts of nitrobacter Winogradsky, 20-30 parts of pseudomonas aeruginosa, 10-20 parts of paracoccus denitrificans, 3-5 parts of beggiatoa, 3-5 parts of thiobacilli, 30-60 parts of infusorian, 30-60 parts of plantlike flagellate, 30-50 parts of dunaliella tertiolecta and 30-50 parts of chlorella vulgaris; then adding to 60-100 parts by weight of beef extract peptone agar mediums for culture. The microbial water purifying agent disclosed by the invention can achieve the purpose of purifying the water inside a culture pond by being added to a loach culture pond.
Owner:江门市乐米生物科技有限公司

Acidithiobacillus thiooxidans and heap leaching technology using same of middle-grade and low-grade phosphate ore

The invention relates to Acidithiobacillus thiooxidans and a heap leaching technology using same of the middle-grade and low-grade phosphate ore. The name of the bacteria is Acidithiobacillus thiooxidans RETECH-PS1; the bacteria are conserved in the China center for type culture collection of which address is the Wuhan University; the conservation date is April 25, 2009; and the CCTCC NO is 209088. The biological heap leaching technology of the middle-grade and low-grade phosphate ore comprises the following steps: ores are crushed to less than 15mm, sulfur is added to mix and build up leaching heap; (2) the Acidithiobacillus thiooxidans in the claim 1 which is conserved at low temperature is activated and propagated step by step to reach the bacterium solution volume for spraying, finally the bacteria are inoculated in the middle-grade and low-grade phosphate ore heap mixed with sulfur; and (3) leachate is collected by a collection pool and pumped by a pump to a spraying system for circular spraying. The invention has the following advantages: the process flow is simple, the middle-grade and low-grade phosphate ore is used without adopting ore dressing and enrichment; sulfuric acid is not added in the production process of phosphoric acid, thus reducing the cost and avoiding the environmental pollution problem caused by the production of sulfuric acid; the production scale can be large or small; and the technology is especially suitable for the collophanite resource which accounts for 70% of Chinese phosphorus resources and is difficult to treat through traditional ore dressing, thus increasing the comprehensive utilization rate of Chinese phosphorus resources.
Owner:有研资源环境技术研究院(北京)有限公司

Microbial water purifying agent for grass shrimp farming water and preparation method of microbial water purifying agent

The invention discloses a microbial water purifying agent for grass shrimp farming wastewater and a preparation method of the microbial water purifying agent. The preparation method comprises the following steps: adding 30-50 parts by weight of magnetic silica beads with surface modification of C8 and C18 alkyl chains into 300-500 parts by weight of water; adding 150-200 parts by weight of a microbial reagent comprising 40-70 parts of rhodomicrobium, 40-50 parts of rhodobactersphacroides, 20-40 parts of nitrosomonas, 20-30 parts of nitrobacterwinogradskyi, 10-30 parts of pseudomonasstutzeri, 20-40 parts of leptothrix, 4-5 parts of thiobacillus, 2-5 parts of beggiatoa, 50-80 parts of nematodes, 30-40 parts of aeolosomatidaes, 20-40 parts of dunaliella and 20-40 parts of chlorella; and further adding 70-90 parts by weight of a beef extract peptone agar culture medium for culture. By putting the microbial water purifying agent disclosed by the invention into a grass shrimp farming pond, the purpose of purifying the water in the farming pond can be achieved.
Owner:青岛科洋水产技术研究有限公司

Composite micro-ecological bacterial agent for improving eutrophication of water body and preparation method thereof

The invention discloses a composite micro-ecological bacterial agent for improving eutrophication of a water body and a preparation method thereof, and belongs to the technical field of microbial fermentation. The composite micro-ecological bacterial agent comprises bacillus subtilis, bacillus megatherium, bacillus aryabhattai, thiobacillus and respectively corresponding bacillus and thiobacillus carrier culture media. According to the composite micro-ecological bacterial agent for reducing eutrophication of the water body, which is provided by the invention, the main research and development direction is to reduce ammonia nitrogen, hydrogen sulfide and organophosphorus in the aquaculture water body and maintain proper water quality, different strain production and drying processes are reasonably prepared, and metabolic characteristics of the bacillus subtilis, the bacillus megatherium, the bacillus aryabhattai and the thiobacillus are integrated, so that the bacillus subtilis, the bacillus megatherium, the bacillus aryabhattai and the thiobacillus can take a synergistic effect, the aims of reducing excessive enrichment of ammonia, nitrogen, sulfur and phosphorus and reducing the risk of environmental pollution are fulfilled, the proper water quality is maintained, and the environmental pollution is reduced.
Owner:ANHUI TECH BANK BIO TECH

Preparation method of thiobacillus denitrificans-embedded high-strength immobilized microspheres

The invention relates to a preparation method of thiobacillus denitrificans-embedded high-strength immobilized microspheres. The preparation method comprises the following steps: (1) obtaining an aqueous solution of thiobacillus denitrificans; (2) adding polyvinyl alcohol and sodium alginate into water, performing heating to change the mixture into a viscous liquid, performing cooling, and then adding a volcanic rock powder into the viscous liquid to obtain a volcanic rock powder modified polyvinyl alcohol composite sodium alginate embedding agent; (3) grinding oyster shells into an oyster powder; (4) mixing the oyster powder obtained in the step (3) with wet thalli obtained in the step (1) to obtain an embedding body for embedding; (5) uniformly mixing the embedding body and the embeddingagent, and dropwise adding the mixture into a cross-linking agent for carrying out a reaction until the mixture is solidified into a sphere shape to obtain immobilized microspheres; and (6) obtainingthe thiobacillus denitrificans-embedded high-strength immobilized microspheres. The immobilized microspheres have relatively high mechanical strength and elasticity, prolong the service life of hydrogel, and have high practical application value.
Owner:HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY +1

A kind of microbial water purifying agent for grass shrimp culture water and preparation method thereof

The invention discloses a microbial water purifying agent used for grass shrimp culture wastewater and a preparation method thereof. The preparation method: add 30-50 parts by weight of magnetic silicon spheres with surface-modified C8 and C18 alkyl chains to 300-500 parts by weight of water; add 150-200 parts by weight of microbial reagents: 40-70 parts of Rhodomicrobacteria, 40-50 parts of Rhodobacter sphaericus, 20-40 parts of Nitrosomonas, 20-30 parts of Nitrobacter victoria, 10-30 parts of Pseudomonas stutzeri, 20-40 parts of Phytophthora, Thiobacillus 4- 5 parts, 2-5 parts of Thiobacillus beinerii, 50-80 parts of nematodes, 30-40 parts of vibrating worms, 20-40 parts of Dunaliella, 20-40 parts of chlorella; then add 70-90 parts by weight of beef extract Cultured on peptone agar medium. Putting the microbial water purifying agent of the present invention into the grass shrimp culture pond can achieve the purpose of purifying the water in the culture pond.
Owner:青岛科洋水产技术研究有限公司

Method for promoting chalcopyrite bio-leaching based on iron-sulfur metabolism regulation

The invention discloses a method for improving efficiency of leaching chalcopyrite from thiobacillus and belongs to the technical field of bioengineering. According to the method disclosed by the invention, on the basis of an improved Starkey-chalcopyrite composite culture medium, iron ions and ferrous ions are supplemented at the initial stage of culture and elemental sulfur is added at stages inthe bio-leaching process, various biochemical reactions in the leaching environment are regulated, chemical and biological factors in the bio-leaching process are enhanced, iron metabolism is promoted while sulfur metabolism is improved, and production of sulfur passivation films and iron passivation films is reduced, so that the leaching rate is improved. The method is simple and feasible in operation, and is applicable to large-scale popularization and application of similar bio-leaching processes.
Owner:JIANGNAN UNIV

Desulfurization microbial agent and sewage treatment technology using same

The invention discloses a desulfurization microbial agent and a sewage treatment technology using the same, and relates to the technical field of sewage biochemical treatment. The desulfurization microbial agent comprises a biological synergistic carrier and a complex microbial inoculant adsorbed on the biological synergistic carrier, and the complex microbial inoculant comprises the following components in parts by weight: 4-8 parts of thiobacillus, 2-6 parts of pseudomonas, 3-5 parts of acinetobacter, 10-26 parts of nutritional ingredients and 0.5-3 parts of a slow-release oxygen agent. Thepreparation method has the advantages of convenience in desulfurization of sewage and reduction of secondary pollution.
Owner:浙江永续环境工程有限公司

Acidithiobacillus thiooxidans and heap leaching technology using same of middle-grade and low-grade phosphate ore

The invention relates to Acidithiobacillus thiooxidans and a heap leaching technology using same of the middle-grade and low-grade phosphate ore. The name of the bacteria is Acidithiobacillus thiooxidans RETECH-PS1; the bacteria are conserved in the China center for type culture collection of which address is the Wuhan University; the conservation date is April 25, 2009; and the CCTCC NO is 209088. The biological heap leaching technology of the middle-grade and low-grade phosphate ore comprises the following steps: ores are crushed to less than 15mm, sulfur is added to mix and build up leaching heap; (2) the Acidithiobacillus thiooxidans in the claim 1 which is conserved at low temperature is activated and propagated step by step to reach the bacterium solution volume for spraying, finally the bacteria are inoculated in the middle-grade and low-grade phosphate ore heap mixed with sulfur; and (3) leachate is collected by a collection pool and pumped by a pump to a spraying system for circular spraying. The invention has the following advantages: the process flow is simple, the middle-grade and low-grade phosphate ore is used without adopting ore dressing and enrichment; sulfuric acid is not added in the production process of phosphoric acid, thus reducing the cost and avoiding the environmental pollution problem caused by the production of sulfuric acid; the production scale can be large or small; and the technology is especially suitable for the collophanite resource which accounts for 70% of Chinese phosphorus resources and is difficult to treat through traditional ore dressing, thus increasing the comprehensive utilization rate of Chinese phosphorus resources.
Owner:有研资源环境技术研究院(北京)有限公司

Microbial agent for promoting growth of oilseed rape and oilseed rape planting method thereof

The invention relates to the technical field of agricultural microorganisms, and particularly discloses a microbial agent for promoting growth of oilseed rape and an oilseed rape planting method thereof. The microbial agent provided by the invention comprises the following bacteria: a) at least one of corynebacterium, paracoccus, chromobacterium, achromobacter, acidithiobacillus, acidovorax, alcaligenes, arthrobacter, bacillus, cupriavidus, derxia, herbaspirillum, hydrogenobacter, hydrogenophaga, pseudomonas, pseudonocardia, rhizobium, rhodococcus, rhodopseudomonas, rhodospirillum, thiocapsa, xanthobacter, flavobacterium and wautersia; B) acetobacter, and c) azospirillum. The microbial agent provided by the invention improves the yield of oilseed rape, and is matched with a specific oilseed rape planting method to increase the yield of oilseed rape per mu, reduce the nitrogen fertilizer demand, reduce harmful germs, restore the number of microorganisms in soil and increase the diversity of the microorganisms.
Owner:GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD

Method for removing heavy metals from sludge

The invention relates to a method for removing heavy metals from sludge. Biological leaching of sludge is carried out by supporting thiobacillus on biochar, and and can efficiently remove a variety ofheavy metals in the sludge by combining with elimination and renewal of hydrops, biochar adsorption and the like with low energy consumption, low cost and no need of calcining and heating. The sludgetreated by the method has a high removal rate of the heavy metals and can be widely used in the preparation of organic fertilizers, biochar, etc., greatly improving the economic and social benefits of sludge treatment.
Owner:成都碧青环保科技有限公司

A microbial carrier of Thiobacillus denitrificans

The invention discloses a microbial carrier of thiobacillus denitrificans. The microbial carrier comprises, by weight percentage, 20-30% of sulfur, 15-30% of sodium thiosulfate, 20-30% of limestone, 0.2-0.5% of potassium dihydrogen phosphate, 0.3-0.5% of magnesium oxide and the balance of adhesive. The microbial carrier has the advantages that the microbial carrier is large in specific surface area, has a large-pore-volume absorption structure and can carry the thiobacillus denitrificans; the thiobacillus denitrificans is an obligate autotrophic denitrifying bacterium and is capable of removing nitrate nitrogen so as to degrade the total nitrogen in polluted water; the microbial carrier does not need to provide an extra energy source needed by the thiobacillus denitrificans, generation of microorganisms other than the thiobacillus denitrificans is avoided, related problems cannot derive, and the microbial carrier using inorganic matter such as the sulfur and carbonate is low in environment burden as compared with microbial carriers using organic matter; equipment used by the microbial carrier is simple in overall structure and low in operation cost and hardly generates sludge.
Owner:东莞市台腾环保材料科技有限公司

Biological preparation capable of decomposing bottom mud and preparation process ofbiological preparation

The invention discloses a biological preparation capable of decomposing bottom mud, and relates to the technical field of microorganisms. The biological preparation comprises a porous carrier, and complex microbial inoculants and enzyme preparations which are located in the carrier and distributed in multiple layers, the porous carrier is composed of multiple isolating layers which are isolated from one another, and multiple complex microbial inoculants or enzyme preparations are attached to the isolating layers respectively. The complex microbial inoculant comprises the following components in percentage by weight: 16-20% of bacillus, 15-17% of lactobacillus plantarum, 12-18% of nitrifying bacteria, 14-18% of photosynthetic bacteria, 11-13% of thiobacillus denitrificans, 9-13% of acetobacter aceti and the balance of an enzyme preparation. The invention further discloses a preparation process of the biological preparation capable of decomposing the bottom mud, the preparation process comprises four steps, in the preparation method of the biological agent, organic raw materials such as rice bran, bran meal and soybean meal are directly utilized for fermentation of various bacteria, and the raw materials are natural raw materials, do not contain pathogenic bacteria and pathogens and do not generate secondary pollution.
Owner:青岛威羽山环保科技有限公司

Biological desulphurization technology

The invention relates to a biological desulphurization technology which can be used for removing SO2 from smoke. SO2 in the smoke is dissolved in water to generate sulfite and sulfate; under the conditions of an anaerobic environment and an external carbon source, sulfate reducing bacteria (SRB) reduces the sulfite and the sulfate into sulfide; and then, under the aerobiotic condition, the sulfide is converted into elemental sulfur under the action of desulfurization microorganism (colorless thiobacillus), precipitating and separating are carried out, and thus, the elemental sulfur is removed from a system. The technology realizes the elemental sulfur recovery through efficiency biological reaction, thus, the SO2 pollution is effectively solved, waste recycling is realized, and the unification of environmental benefit and economic benefit is finally realized.
Owner:天津市庆安环境检测有限公司

Enrichment culture method of sulfur bacteria

The invention discloses an enrichment culture method of sulfur bacteria. The method comprises the following steps of (1) determining an upper limit value DOmax of the DO concentration in a culture system and a corresponding aeration rate Q; (2) determining the dissolved oxygen concentration, recorded as DOtop, of sulfur bacteria in the optimal growth state period; (3) in a sulfur bacteria culture process, when the DO concentration is increased to 30%-40% of DOmax, starting a material supplementing system; when the DO is reduced by 15-25%, stopping the material supplementing system; when the dissolved oxygen concentration is lower than 2% of DOmax and stays for 10-20 minutes, discharging part of bacteria-water mixture according to 5%-10% of the total volume of a bacteria-water mixture in the culture system; and repeating the steps until a culture process is finished. According to the method, the automatic control culture of bacterial cells can be realized, the rapid growth and propagation can be realized, the activity of the obtained sulfur bacteria is high, the rapid growth and propagation of thiobacillus of which a final product is elemental sulfur can be realized, and a new pollution source cannot be generated while sulfide pollution of a sewage treatment plant is solved.
Owner:CHINA PETROLEUM & CHEM CORP +1

Microbiological water purifying agent for ricefield eel breeding water and preparation method of microbiological water purifying agent

InactiveCN104276668ASuitable for online processingEasy to settleBiological water/sewage treatmentBiotechnologyNematode
The invention discloses a microbiological water purifying agent for ricefield eel breeding water and a preparation method of the microbiological water purifying agent. The preparation method comprises the steps of adding 30-50 parts by weight of magnetic silica spheres with C8 and C18 alkyl chains modified on surfaces into 150-250 parts by weight of water; adding 150-250 parts by weight of microbiological reagents including 40-50 parts of rhodospirillum, 40-60 parts of rhodopseudomonas, 30-50 parts of Ni-trosolobus, 30-40 parts of nitrococcus, 20-30 parts of thiobacillus denitrificans, 30-40 parts of sphaerotilus natans, 2-4 parts of vitreoscilla, 2-4 parts of thiobacillus, 30-50 parts of paramecium, 40-60 parts of nematode, 40-50 parts of platymonas subcordiformis and 40-50 parts of cyanobacteria; and adding 60-80 parts by weight of beef extract peptone agar, and culturing in a culture medium. The aim of purifying water in a breeding pond can be achieved after the microbiological water purifying agent disclosed by the invention is added into a ricefield eel breeding pond.
Owner:SHANGHAI KUIYA ENVIRONMENTAL PROTECTION TECH

Method for separating sulfide from lignin

InactiveCN104448335AOther features have no effectBiotechnologyMicroorganism
The invention relates to a method for separating sulfide from lignin. The method is characterized by comprising the following steps: adding lignin and thiobacillus into a reaction kettle at the ratio of 5 to 1; stirring and reacting for 2 hours to form yellow particles; and participating and separating the reacted mixture. According to the method disclosed by the invention, sulphur is removed with microorganisms; the other characteristics of the lignin are not affected; no other chemical residues exist; and the lignin from which sulfide is removed can be applied to the industries such as medicines and foods.
Owner:徐娟

A kind of bioselective leaching method of low-grade copper-cobalt ore

The invention discloses a biological selectivity leaching method of low-grade copper-cobalt ore, and belongs to the field of microbial metallurgy. The biological selectivity leaching method comprises the following steps: the copper-cobalt ore is crushed and grinded; a mixed bacterium liquid containing ferrous oxide micro spirilla, acidophilic thiobacillus ferrooxidans and acidophilic thiobacillus thiooxidans is introduced to a 9 K of culture medium for cultivating to obtain a bacterium culture solution. The copper-cobalt ore is added to the bacterium culture solution to conduct bacterium leaching on the cobalt concentrate; when the leaching rate of cobalt in the bacterium leaching liquid reaches above 97%, the leaching liquid containing valuable metal ions such as cobalt and copper is separated from the leaching residue; then Lix984N extraction agent is adopted to extract copper to obtain a copper product; the raffinate from which the copper is extracted is subjected to precipitation and iron removing treatment by a jarosite process to obtain an iron product; cobalt-containing liquid after being subjected to iron removal is crystallized to obtain a cobalt oxalate product. The biological selectivity leaching method has the advantages of short technique flow, easy operation, simple equipment, low energy consumption, no high-temperature smelting, no emission of pollutant smoke and poisonous gas, and quick achievement of separation and efficient recovery of cobalt and other valuable metals.
Owner:NORTHEASTERN UNIV LIAONING

Bacterial strain for degrading thiocyanate, composite bacterial strain, microbial inoculum and application of bacterial strain, composite bacterial strain and microbial inoculum

The invention discloses a bacterial strain for degrading thiocyanate, a compound bacterial strain, a bacterial agent and application of the bacterial strain, the compound bacterial strain and the bacterial agent. The compound bacterial strain comprises lysinibacillus sp. S3, Acinetobacter sifertii LB3, Thiobacillus sp. A, Pseudomonas sp. WU6 and Acinetobacter bayi WU9, and the compound bacterial strain is prepared from bacillus amyloliquefaciens sp.S3, Acinetobacter sifertii LB3, Thiobacillus sp. A, Pseudomonas sp. WU6 and Acinetobacter bayi WU9. Specific strains are compounded together, so that the synergistic interaction of the strains is realized, the thiocyanate in the wastewater is efficiently degraded, the treatment efficiency of the thiocyanate-containing wastewater is remarkably improved, and the degradation period of the thiocyanate-containing wastewater is effectively shortened. Specifically, the compound strain can effectively degrade the industrial wastewater with the thiocyanate concentration of 3000mg / L within 36 hours, and the degradation method is high in degradation efficiency, short in period, stable in operation and capable of better meeting market requirements.
Owner:NANCHANG GUOCHANG ENVIRONMENTAL PROTECTION TECH +1
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