59 results about "Thiobacillus sp." patented technology

The invention relates to Acidithiobacillus thiooxidans and a heap leaching technology using same of the middle-grade and low-grade phosphate ore. The name of the bacteria is Acidithiobacillus thiooxidans RETECH-PS1; the bacteria are conserved in the China center for type culture collection of which address is the Wuhan University; the conservation date is April 25, 2009; and the CCTCC NO is 209088. The biological heap leaching technology of the middle-grade and low-grade phosphate ore comprises the following steps: ores are crushed to less than 15mm, sulfur is added to mix and build up leaching heap; (2) the Acidithiobacillus thiooxidans in the claim 1 which is conserved at low temperature is activated and propagated step by step to reach the bacterium solution volume for spraying, finally the bacteria are inoculated in the middle-grade and low-grade phosphate ore heap mixed with sulfur; and (3) leachate is collected by a collection pool and pumped by a pump to a spraying system for circular spraying. The invention has the following advantages: the process flow is simple, the middle-grade and low-grade phosphate ore is used without adopting ore dressing and enrichment; sulfuric acid is not added in the production process of phosphoric acid, thus reducing the cost and avoiding the environmental pollution problem caused by the production of sulfuric acid; the production scale can be large or small; and the technology is especially suitable for the collophanite resource which accounts for 70% of Chinese phosphorus resources and is difficult to treat through traditional ore dressing, thus increasing the comprehensive utilization rate of Chinese phosphorus resources.

The invention discloses a microbial carrier of thiobacillus denitrificans. The microbial carrier comprises, by weight percentage, 20-30% of sulfur, 15-30% of sodium thiosulfate, 20-30% of limestone, 0.2-0.5% of potassium dihydrogen phosphate, 0.3-0.5% of magnesium oxide and the balance of adhesive. The microbial carrier has the advantages that the microbial carrier is large in specific surface area, has a large-pore-volume absorption structure and can carry the thiobacillus denitrificans; the thiobacillus denitrificans is an obligate autotrophic denitrifying bacterium and is capable of removing nitrate nitrogen so as to degrade the total nitrogen in polluted water; the microbial carrier does not need to provide an extra energy source needed by the thiobacillus denitrificans, generation of microorganisms other than the thiobacillus denitrificans is avoided, related problems cannot derive, and the microbial carrier using inorganic matter such as the sulfur and carbonate is low in environment burden as compared with microbial carriers using organic matter; equipment used by the microbial carrier is simple in overall structure and low in operation cost and hardly generates sludge.

The invention discloses a biological selectivity leaching method of low-grade copper-cobalt ore, and belongs to the field of microbial metallurgy. The biological selectivity leaching method comprises the following steps: the copper-cobalt ore is crushed and grinded; a mixed bacterium liquid containing ferrous oxide micro spirilla, acidophilic thiobacillus ferrooxidans and acidophilic thiobacillus thiooxidans is introduced to a 9 K of culture medium for cultivating to obtain a bacterium culture solution. The copper-cobalt ore is added to the bacterium culture solution to conduct bacterium leaching on the cobalt concentrate; when the leaching rate of cobalt in the bacterium leaching liquid reaches above 97%, the leaching liquid containing valuable metal ions such as cobalt and copper is separated from the leaching residue; then Lix984N extraction agent is adopted to extract copper to obtain a copper product; the raffinate from which the copper is extracted is subjected to precipitation and iron removing treatment by a jarosite process to obtain an iron product; cobalt-containing liquid after being subjected to iron removal is crystallized to obtain a cobalt oxalate product. The biological selectivity leaching method has the advantages of short technique flow, easy operation, simple equipment, low energy consumption, no high-temperature smelting, no emission of pollutant smoke and poisonous gas, and quick achievement of separation and efficient recovery of cobalt and other valuable metals.

The invention provides a thiobacillus acidophilus, the name of the strain is: Thiobacillus ferrooxidans ST-A, the depository unit is China National Center for Typical Culture Collection, address: Wuhan University, deposit date: May 24, 2015, deposit registration number : CCTCC NO: M2015333. Using this bacteria to treat iron-containing mine acid wastewater can accelerate the oxidation of ferrous ions to generate ferric ions at low pH; at a suitable pH, the bacteria can catalyze ferric ions to form Shi's minerals (Fe 8 o 8 (OH) 6 (SO 4 )) Precipitation, effective recovery of iron resources, and the recovered iron-containing precipitates will not entrain other valuable metals (copper, zinc, etc.), providing an operational process for the recovery of metal resources in mine acid wastewater.