58 results about "Thiobacillus sp." patented technology

The instant invention presents means for sequestering COx and NOx; further comprising algae means to convert COx into oxygen (O2), as well as biological means to convert sulfides into elemental sulfur. The instant invention comprises algae, heterotrophs, facultative bacteria and Thiobacillus. The instant invention comprises means of light (photon) transfer. Fiber optics is a means of photon transfer to provide photons to a biological reactor. The instant invention comprises the photon depth adsorption capability of algae in biological reactor means. The instant invention comprises means of energy management so that the instant invention may be used in most any environment, wherein a photon (light) source is available and can comprise a means of photon source generation when a light source is not available.

The invention discloses a functional material for removing nitrate nitrogen in water and preparation and application methods thereof. The functional material is obtained by taking sulfur powder and high-activity iron-manganese sulfide powder as main raw materials and taking biomass fibers and a binder as additives, uniformly mixing the raw materials, adding water for molding, and then curing to finish hydration or carbonation. mixing functional material and limestone or dolomite particles and then fillinginto a biological filter, inoculating thiobacillus and culturing until a biological membrane is mature, and treating the wastewater in an upwelling mode according to the hydraulic retention time of 1-5h so that the removal rate of nitrate nitrogen in the wastewater reaches 80% or above.

The invention relates to Acidithiobacillus thiooxidans and a heap leaching technology using same of the middle-grade and low-grade phosphate ore. The name of the bacteria is Acidithiobacillus thiooxidans RETECH-PS1; the bacteria are conserved in the China center for type culture collection of which address is the Wuhan University; the conservation date is April 25, 2009; and the CCTCC NO is 209088. The biological heap leaching technology of the middle-grade and low-grade phosphate ore comprises the following steps: ores are crushed to less than 15mm, sulfur is added to mix and build up leaching heap; (2) the Acidithiobacillus thiooxidans in the claim 1 which is conserved at low temperature is activated and propagated step by step to reach the bacterium solution volume for spraying, finally the bacteria are inoculated in the middle-grade and low-grade phosphate ore heap mixed with sulfur; and (3) leachate is collected by a collection pool and pumped by a pump to a spraying system for circular spraying. The invention has the following advantages: the process flow is simple, the middle-grade and low-grade phosphate ore is used without adopting ore dressing and enrichment; sulfuric acid is not added in the production process of phosphoric acid, thus reducing the cost and avoiding the environmental pollution problem caused by the production of sulfuric acid; the production scale can be large or small; and the technology is especially suitable for the collophanite resource which accounts for 70% of Chinese phosphorus resources and is difficult to treat through traditional ore dressing, thus increasing the comprehensive utilization rate of Chinese phosphorus resources.

The invention discloses a composite micro-ecological bacterial agent for improving eutrophication of a water body and a preparation method thereof, and belongs to the technical field of microbial fermentation. The composite micro-ecological bacterial agent comprises bacillus subtilis, bacillus megatherium, bacillus aryabhattai, thiobacillus and respectively corresponding bacillus and thiobacillus carrier culture media. According to the composite micro-ecological bacterial agent for reducing eutrophication of the water body, which is provided by the invention, the main research and development direction is to reduce ammonia nitrogen, hydrogen sulfide and organophosphorus in the aquaculture water body and maintain proper water quality, different strain production and drying processes are reasonably prepared, and metabolic characteristics of the bacillus subtilis, the bacillus megatherium, the bacillus aryabhattai and the thiobacillus are integrated, so that the bacillus subtilis, the bacillus megatherium, the bacillus aryabhattai and the thiobacillus can take a synergistic effect, the aims of reducing excessive enrichment of ammonia, nitrogen, sulfur and phosphorus and reducing the risk of environmental pollution are fulfilled, the proper water quality is maintained, and the environmental pollution is reduced.

The invention discloses a microbial water purifying agent used for grass shrimp culture wastewater and a preparation method thereof. The preparation method: add 30-50 parts by weight of magnetic silicon spheres with surface-modified C8 and C18 alkyl chains to 300-500 parts by weight of water; add 150-200 parts by weight of microbial reagents: 40-70 parts of Rhodomicrobacteria, 40-50 parts of Rhodobacter sphaericus, 20-40 parts of Nitrosomonas, 20-30 parts of Nitrobacter victoria, 10-30 parts of Pseudomonas stutzeri, 20-40 parts of Phytophthora, Thiobacillus 4- 5 parts, 2-5 parts of Thiobacillus beinerii, 50-80 parts of nematodes, 30-40 parts of vibrating worms, 20-40 parts of Dunaliella, 20-40 parts of chlorella; then add 70-90 parts by weight of beef extract Cultured on peptone agar medium. Putting the microbial water purifying agent of the present invention into the grass shrimp culture pond can achieve the purpose of purifying the water in the culture pond.

The invention discloses a microbial carrier of thiobacillus denitrificans. The microbial carrier comprises, by weight percentage, 20-30% of sulfur, 15-30% of sodium thiosulfate, 20-30% of limestone, 0.2-0.5% of potassium dihydrogen phosphate, 0.3-0.5% of magnesium oxide and the balance of adhesive. The microbial carrier has the advantages that the microbial carrier is large in specific surface area, has a large-pore-volume absorption structure and can carry the thiobacillus denitrificans; the thiobacillus denitrificans is an obligate autotrophic denitrifying bacterium and is capable of removing nitrate nitrogen so as to degrade the total nitrogen in polluted water; the microbial carrier does not need to provide an extra energy source needed by the thiobacillus denitrificans, generation of microorganisms other than the thiobacillus denitrificans is avoided, related problems cannot derive, and the microbial carrier using inorganic matter such as the sulfur and carbonate is low in environment burden as compared with microbial carriers using organic matter; equipment used by the microbial carrier is simple in overall structure and low in operation cost and hardly generates sludge.

The invention relates to a biological desulphurization technology which can be used for removing SO2 from smoke. SO2 in the smoke is dissolved in water to generate sulfite and sulfate; under the conditions of an anaerobic environment and an external carbon source, sulfate reducing bacteria (SRB) reduces the sulfite and the sulfate into sulfide; and then, under the aerobiotic condition, the sulfide is converted into elemental sulfur under the action of desulfurization microorganism (colorless thiobacillus), precipitating and separating are carried out, and thus, the elemental sulfur is removed from a system. The technology realizes the elemental sulfur recovery through efficiency biological reaction, thus, the SO2 pollution is effectively solved, waste recycling is realized, and the unification of environmental benefit and economic benefit is finally realized.

The invention discloses an enrichment culture method of sulfur bacteria. The method comprises the following steps of (1) determining an upper limit value DOmax of the DO concentration in a culture system and a corresponding aeration rate Q; (2) determining the dissolved oxygen concentration, recorded as DOtop, of sulfur bacteria in the optimal growth state period; (3) in a sulfur bacteria culture process, when the DO concentration is increased to 30%-40% of DOmax, starting a material supplementing system; when the DO is reduced by 15-25%, stopping the material supplementing system; when the dissolved oxygen concentration is lower than 2% of DOmax and stays for 10-20 minutes, discharging part of bacteria-water mixture according to 5%-10% of the total volume of a bacteria-water mixture in the culture system; and repeating the steps until a culture process is finished. According to the method, the automatic control culture of bacterial cells can be realized, the rapid growth and propagation can be realized, the activity of the obtained sulfur bacteria is high, the rapid growth and propagation of thiobacillus of which a final product is elemental sulfur can be realized, and a new pollution source cannot be generated while sulfide pollution of a sewage treatment plant is solved.

The invention discloses a biological selectivity leaching method of low-grade copper-cobalt ore, and belongs to the field of microbial metallurgy. The biological selectivity leaching method comprises the following steps: the copper-cobalt ore is crushed and grinded; a mixed bacterium liquid containing ferrous oxide micro spirilla, acidophilic thiobacillus ferrooxidans and acidophilic thiobacillus thiooxidans is introduced to a 9 K of culture medium for cultivating to obtain a bacterium culture solution. The copper-cobalt ore is added to the bacterium culture solution to conduct bacterium leaching on the cobalt concentrate; when the leaching rate of cobalt in the bacterium leaching liquid reaches above 97%, the leaching liquid containing valuable metal ions such as cobalt and copper is separated from the leaching residue; then Lix984N extraction agent is adopted to extract copper to obtain a copper product; the raffinate from which the copper is extracted is subjected to precipitation and iron removing treatment by a jarosite process to obtain an iron product; cobalt-containing liquid after being subjected to iron removal is crystallized to obtain a cobalt oxalate product. The biological selectivity leaching method has the advantages of short technique flow, easy operation, simple equipment, low energy consumption, no high-temperature smelting, no emission of pollutant smoke and poisonous gas, and quick achievement of separation and efficient recovery of cobalt and other valuable metals.

The invention provides a thiobacillus acidophilus, the name of the strain is: Thiobacillus ferrooxidans ST-A, the depository unit is China National Center for Typical Culture Collection, address: Wuhan University, deposit date: May 24, 2015, deposit registration number : CCTCC NO: M2015333. Using this bacteria to treat iron-containing mine acid wastewater can accelerate the oxidation of ferrous ions to generate ferric ions at low pH; at a suitable pH, the bacteria can catalyze ferric ions to form Shi's minerals (Fe 8 o 8 (OH) 6 (SO 4 )) Precipitation, effective recovery of iron resources, and the recovered iron-containing precipitates will not entrain other valuable metals (copper, zinc, etc.), providing an operational process for the recovery of metal resources in mine acid wastewater.

The invention discloses a strain of acidophilus thermophilic thiobacterium and its application, in particular to a kind of acidophilus thermophilic thiobacterium with dual metal resistance and a method for biocatalytically leaching chalcopyrite by using the strain, which belongs to the field of bioengineering . The invention domesticates the original mesophilic acidophilic thiobacterium, continuously improves its copper-resistant performance through gradient domestication, leaches the copper-resistant strain and the original strain under different copper stress concentrations, and verifies the improvement of the leaching level of the domesticated strain under various conditions. The domesticated strains are leached in a copper stress environment, and silver ions are added to the leaching system to promote the interaction between ions, which makes the leaching system active, promotes iron-sulfur metabolism, and reduces the formation of passive film. The environment quickly adapted, and finally the copper ion leaching rate increased to 54.71%. The method is simple and easy to operate, low in cost, and suitable for large-scale popularization and application of similar bioleaching processes.