Thiobacillus acidophilus and method for treating acid mine wastewater and recycling iron resource by adopting same

A technology of acidophilus thiobacillus and strains, applied in the field of microorganisms, can solve the problems of unrecoverable waste water iron and other valuable metals, secondary pollution, large amount of slag, etc.

Active Publication Date: 2017-06-20
有研资源环境技术研究院(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the conventional treatment method is lime neutralization method, which produces a large amount of slag, cannot recover iron and other valuable metals in wastewater, and is prone to secondary pollution

Method used

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  • Thiobacillus acidophilus and method for treating acid mine wastewater and recycling iron resource by adopting same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Acquisition and Identification of Acidophilus Thiobacillus

[0033] The obtaining method of acidic thiobacillus acidophilus provided by the invention is:

[0034] 1) Add 0.3g of yeast powder to 100mL of the water sample containing the acidic thiobacillus acidophilus, enrich and culture at 30°C for 1 week with gentle shaking on a shaker at 100rpm, and use a microscope to detect the growth of the bacteria; the water sample is taken from Jiangxi De Xing copper mine copper-containing acid wastewater. Bacteria grow to measure the absorbance value of the bacterial culture solution at 600nm, if the obtained OD600 value is between 0.6-0.8.

[0035] 2) Filter the bacteria in the above culture solution by membrane filtration, and wash the bacteria with 20 mL of sterile water. Inoculate multiple 100mL enrichment media with 5% inoculum amount, culture at 45°C, and set no inoculation control (CK). The growth of bacteria was observed after two weeks of culture on a shake...

Embodiment 2

[0039] Example 2 Treatment of Mine Acidic Wastewater and Recovery of Iron Resources

[0040] 1) The acid wastewater from the Dexing Copper Mine in Jiangxi is passed into a biological oxidation reactor, and the biological oxidation reactor is filled with commercially available soft packing for water treatment.

[0041] The wastewater quality is as follows: pH2.3; Cu 2+ : 220mg / L, total iron ion concentration: 1480mg / L.

[0042] 2) Inoculate the identified bacterial species of Acidophilus thiobacillus into the enrichment medium, and culture it on a shaker at 45°C at 100rpm until the bacterial concentration is 10 8 each / mL; then inoculate the bacterial solution into the biooxidation reactor with an inoculum volume of 3L / m 3 .

[0043] The biooxidation reactor used is a conventional packed biofilm reactor with air aeration at the bottom, and the treatment time is 1 hour.

[0044] 3) After the reaction, the effluent was passed into the solid-liquid separation reactor, the pH wa...

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Abstract

The invention provides thiobacillus acidophilus. The name of the strain is Thiobacillus ferrooxidans ST-A, the Thiobacillus ferrooxidans ST-A is preserved in the China Center for Type Culture Collection (located in Wuhan University) on May 24, 2015, and is assigned with the accession number of CCTCC NO:M2015333. When the strain is adopted for treating iron-containing acid mine wastewater, divalent iron ions are oxidized in an acceleration manner under low pH, and then trivalent iron ions are generated; under the suitable pH, the strain can catalyze the trivalent iron ions to form schwertmannite (Fe8O8(OH)6(SO4)) sediment, so that the iron resource is effectively recycled, meanwhile, the recycled iron-containing sediment does not contain other valuable metal (copper, zinc and the like), and thus an operable process is provided for the resource recycling of metal in the acid mine wastewater.

Description

technical field [0001] The invention relates to the technical field of microorganisms. In particular, it relates to a bacterium of the genus Thiobacillus, its cultivation method and the process of using the bacterium to treat acid waste water from iron-containing mines and recover iron resources. Background technique [0002] Sulfide mines all have the problem of mine acid wastewater. As long as the pyrite is exposed to the air or the groundwater containing dissolved oxygen contacts the ore, the ore will slowly oxidize to produce ferrous ions. At this time, due to the high pH, ​​ferrous ions can be rapidly oxidized into ferric ions, and the presence of ferric ions will accelerate the oxidation process of pyrite and cause the pH to drop rapidly. When the pH further drops to 3.5 Next, under the catalysis of microorganisms, ferric ions will be continuously produced, which will further increase the oxidation rate of pyrite and other sulfide ores, and accelerate the production o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12P3/00C12R1/01C02F103/10C02F101/20
CPCC02F3/346C02F2101/203C02F2103/10C12N1/20C12P3/00C12N1/205C12R2001/01
Inventor 刘兴宇刘文彦陈勃伟张明江温建康
Owner 有研资源环境技术研究院(北京)有限公司
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