Preparation method of thiobacillus denitrificans-embedded high-strength immobilized microspheres

Abstract

The invention relates to a preparation method of thiobacillus denitrificans-embedded high-strength immobilized microspheres. The preparation method comprises the following steps: (1) obtaining an aqueous solution of thiobacillus denitrificans; (2) adding polyvinyl alcohol and sodium alginate into water, performing heating to change the mixture into a viscous liquid, performing cooling, and then adding a volcanic rock powder into the viscous liquid to obtain a volcanic rock powder modified polyvinyl alcohol composite sodium alginate embedding agent; (3) grinding oyster shells into an oyster powder; (4) mixing the oyster powder obtained in the step (3) with wet thalli obtained in the step (1) to obtain an embedding body for embedding; (5) uniformly mixing the embedding body and the embeddingagent, and dropwise adding the mixture into a cross-linking agent for carrying out a reaction until the mixture is solidified into a sphere shape to obtain immobilized microspheres; and (6) obtainingthe thiobacillus denitrificans-embedded high-strength immobilized microspheres. The immobilized microspheres have relatively high mechanical strength and elasticity, prolong the service life of hydrogel, and have high practical application value.

Description

technical field [0001] The invention belongs to the field of waste water treatment, and in particular relates to a preparation method of high-strength immobilized microspheres embedding Thiobacillus denitrificans. Background technique [0002] Autotrophic denitrification has gradually become the focus of current research, and in autotrophic denitrification, sulfur autotrophic denitrification has a certain representativeness. It utilizes Thiobacillus denitrificans and uses elemental sulfur as an electron donor under anoxic conditions. NO 3 --N acts as an electron acceptor to realize the process of denitrification and denitrification. Compared with the traditional denitrification using heterotrophic facultative anaerobic bacteria, it has very little sludge production when dealing with low C / N sewage , No need to add carbon source, low operating cost. However, the sulfur autotrophic denitrification technology still faces many new challenges, such as low efficiency, easy loss o...

AI Technical Summary

Problems solved by technology

However, sulfur autotrophic denitrification technology still faces many new challenges, such as low efficiency, easy loss of bacteria, and sensitivity to changes in temperature and pH, etc.

The application of microbial embedding and immobilization technology in wastewater treatment has the advantages of high biomass, obvious dominant strains, less loss, less land occupation of treatment equipment, and low sludge production, which can make the system obtain better results under harsher conditions. However, traditional microbial embedding such as sodium alginate embedding has the disadvantages of low mechanical strength, poor degradation resistance, and large void size in the gel network, which is not suitable for the embedding of various microorganisms; polyvinyl alcohol embedding has the disadvantages of It is difficult to form, prone to adhesion and has a large water swelling in practical applications, which will lead to the disadvantage of greatly weakening the strength over time

Images