Preparation method of thiobacillus denitrificans-embedded high-strength immobilized microspheres

A high-strength technology of Thiobacillus denitrificans, applied in biochemical equipment and methods, immobilized enzymes, and microbial-based methods, can solve problems such as low mechanical strength, weakened strength, and swelling in large water, and achieve good affinity Water-based and bio-affinity, improve mechanical strength and elasticity, and enhance service life

Active Publication Date: 2020-12-01
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, sulfur autotrophic denitrification technology still faces many new challenges, such as low efficiency, easy loss of bacteria, and sensitivity to changes in temperature and pH, etc.
The application of microbial embedding and immobilization technology in wastewater treatment has the advantages of high biomass, obvious dominant strains, less loss, less land occupation of treatment equipment, and low sludge production, which can make the system obtain better results under harsher condition

Method used

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  • Preparation method of thiobacillus denitrificans-embedded high-strength immobilized microspheres

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Effect test

Embodiment 1

[0032] The invention provides a method for preparing high-strength immobilized microspheres embedding Thiobacillus denitrificans, which comprises the following steps,

[0033] It includes the following steps,

[0034] (1) Inoculate Thiobacillus denitrificans into an aqueous medium for culture to obtain an aqueous solution of Thiobacillus denitrificans, and the concentration of Thiobacillus denitrificans in the obtained aqueous solution of Thiobacillus denitrificans is greater than 10 8 CFU / ml, and then centrifuged to obtain wet cells;

[0035] In step (1), inoculate Thiobacillus denitrificans into the medium, the inoculum amount of Thiobacillus denitrificans is 10%, adjust the pH of the medium to 7.0, sterilize at 121°C for 30min, control the temperature at 30°C, and cultivate 20h, the concentration of Thiobacillus denitrificans in the obtained aqueous solution of Thiobacillus denitrificans was greater than 10 8 CFU / ml, finally centrifuged to obtain wet cells;

[0036] The ...

Embodiment 2

[0052] The invention provides a method for preparing high-strength immobilized microspheres embedding Thiobacillus denitrificans, which comprises the following steps,

[0053] It includes the following steps,

[0054] (1) Inoculate Thiobacillus denitrificans into an aqueous medium for culture to obtain an aqueous solution of Thiobacillus denitrificans, and the concentration of Thiobacillus denitrificans in the obtained aqueous solution of Thiobacillus denitrificans is greater than 10 8 CFU / ml, and then centrifuged to obtain wet cells;

[0055] In step (1), inoculate Thiobacillus denitrificans into the medium, the inoculum amount of Thiobacillus denitrificans is 10%, adjust the pH of the medium to 7.0, sterilize at 121°C for 30min, control the temperature at 32°C, and cultivate 12h, the concentration of Thiobacillus denitrificans in the obtained aqueous solution of Thiobacillus denitrificans was greater than 10 8 CFU / ml, finally centrifuged to obtain wet cells;

[0056] The ...

Embodiment 3

[0071] The invention provides a method for preparing high-strength immobilized microspheres embedding Thiobacillus denitrificans, which comprises the following steps,

[0072] It includes the following steps,

[0073] (1) Inoculate Thiobacillus denitrificans into an aqueous medium for culture to obtain an aqueous solution of Thiobacillus denitrificans, and the concentration of Thiobacillus denitrificans in the obtained aqueous solution of Thiobacillus denitrificans is greater than 10 8 CFU / ml, and then centrifuged to obtain wet cells;

[0074] In step (1), inoculate Thiobacillus denitrificans into the medium, the inoculum amount of Thiobacillus denitrificans is 10%, adjust the pH of the medium to 7.0, sterilize at 121°C for 30min, control the temperature at 28°C, and cultivate 24h, the concentration of Thiobacillus denitrificans in the obtained aqueous solution of Thiobacillus denitrificans is greater than 10 8 CFU / ml, finally centrifuged to obtain wet cells;

[0075] The m...

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Abstract

The invention relates to a preparation method of thiobacillus denitrificans-embedded high-strength immobilized microspheres. The preparation method comprises the following steps: (1) obtaining an aqueous solution of thiobacillus denitrificans; (2) adding polyvinyl alcohol and sodium alginate into water, performing heating to change the mixture into a viscous liquid, performing cooling, and then adding a volcanic rock powder into the viscous liquid to obtain a volcanic rock powder modified polyvinyl alcohol composite sodium alginate embedding agent; (3) grinding oyster shells into an oyster powder; (4) mixing the oyster powder obtained in the step (3) with wet thalli obtained in the step (1) to obtain an embedding body for embedding; (5) uniformly mixing the embedding body and the embeddingagent, and dropwise adding the mixture into a cross-linking agent for carrying out a reaction until the mixture is solidified into a sphere shape to obtain immobilized microspheres; and (6) obtainingthe thiobacillus denitrificans-embedded high-strength immobilized microspheres. The immobilized microspheres have relatively high mechanical strength and elasticity, prolong the service life of hydrogel, and have high practical application value.

Description

technical field [0001] The invention belongs to the field of waste water treatment, and in particular relates to a preparation method of high-strength immobilized microspheres embedding Thiobacillus denitrificans. Background technique [0002] Autotrophic denitrification has gradually become the focus of current research, and in autotrophic denitrification, sulfur autotrophic denitrification has a certain representativeness. It utilizes Thiobacillus denitrificans and uses elemental sulfur as an electron donor under anoxic conditions. NO 3 --N acts as an electron acceptor to realize the process of denitrification and denitrification. Compared with the traditional denitrification using heterotrophic facultative anaerobic bacteria, it has very little sludge production when dealing with low C / N sewage , No need to add carbon source, low operating cost. However, the sulfur autotrophic denitrification technology still faces many new challenges, such as low efficiency, easy loss o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/084C12N11/10C12N11/14C12R1/01
CPCC12N1/20C12N11/084C12N11/10C12N11/14
Inventor 刘艳芳刘晓帅高玮李再兴吕建伟吕永涛
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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