Enrichment culture method of sulfur bacteria

A technology of enrichment culture and sulfur bacteria, applied in the field of environmental microorganisms, can solve the problems such as failure to achieve automatic control of rapid culture and bacterial growth effects, and achieve the effect of solving substrate inhibition, salt concentration will not increase, and yield increase

Active Publication Date: 2021-05-04
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method realizes the high activity culture of Thiobacillus thioexhausen, the main matrix sulfide in the culture solution is a one-time high concentration (the concentration of S2- is greater than 1800mg / L), and the high concentration The S2- is bound to have a certain impact on the growth of bacteria, and at the same time, it has not achieved automatic control and rapid cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 The cultivation of sulfur bacteria

[0031] The sulfur bacteria were cultivated in a 100L aerated reactor. The reactor is equipped with stirring, pH and feeding automatic control system and bacteria-water mixture discharge control system at the same time. During the cultivation process, the temperature was set at 28° C., and the pH was set at 6.8.

[0032](1) First, add 10L of microbial initial culture solution into the aeration reactor, turn on the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit of DO concentration DOmax is 7.1 mg / L and the corresponding aeration rate, and the aeration rate is kept constant throughout the culture process. The composition and content of the microbial culture medium are: sodium sulfide 200mg / L, potassium dihydrogen phosphate 150mg / L, dipotassium hydrogen phospha...

Embodiment 2

[0036] Embodiment 2 The cultivation of sulfur bacteria

[0037] The sulfur bacteria were cultivated in a 100L aerated reactor. The reactor is equipped with stirring, pH and feeding automatic control system and bacteria-water mixture discharge control system at the same time. During the cultivation process, the temperature was set at 25°C and the pH was set at 7.0.

[0038] (1) First add 15L of microbial initial culture solution into the aeration reactor, turn on the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit of DO concentration DOmax is 7.1 mg / L and the corresponding aeration rate, and the aeration rate is kept constant throughout the culture process. The composition and content of the microbial culture medium are: sodium sulfide 200mg / L, potassium dihydrogen phosphate 150mg / L, dipotassium hydrogen phosphate ...

Embodiment 3

[0043] The sulfur bacteria were cultivated in a 100L aerated reactor. The reactor is equipped with stirring, pH and feeding automatic control system and bacteria-water mixture discharge control system at the same time. During the cultivation process, the temperature was set at 25°C and the pH was set at 7.0.

[0044] (1) First add 20L of microbial initial culture solution into the aeration reactor, open the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit of DO concentration DOmax is 7.1 mg / L and the corresponding aeration rate, and the aeration rate is kept constant throughout the culture process. The composition and content of the microbial culture medium are: sodium sulfide 200mg / L, potassium dihydrogen phosphate 150mg / L, dipotassium hydrogen phosphate 150mg / L, magnesium sulfate 150mg / L, ammonium chloride 30mg / L,...

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Abstract

The invention discloses an enrichment culture method of sulfur bacteria. The method comprises the following steps of (1) determining an upper limit value DOmax of the DO concentration in a culture system and a corresponding aeration rate Q; (2) determining the dissolved oxygen concentration, recorded as DOtop, of sulfur bacteria in the optimal growth state period; (3) in a sulfur bacteria culture process, when the DO concentration is increased to 30%-40% of DOmax, starting a material supplementing system; when the DO is reduced by 15-25%, stopping the material supplementing system; when the dissolved oxygen concentration is lower than 2% of DOmax and stays for 10-20 minutes, discharging part of bacteria-water mixture according to 5%-10% of the total volume of a bacteria-water mixture in the culture system; and repeating the steps until a culture process is finished. According to the method, the automatic control culture of bacterial cells can be realized, the rapid growth and propagation can be realized, the activity of the obtained sulfur bacteria is high, the rapid growth and propagation of thiobacillus of which a final product is elemental sulfur can be realized, and a new pollution source cannot be generated while sulfide pollution of a sewage treatment plant is solved.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, in particular to a method for enriching and culturing sulfur bacteria. Background technique [0002] Autotrophic microorganisms include photoautotrophs and chemoautotrophs. Among them, many bacteria in the sewage treatment process belong to chemoautotrophic microorganisms, which mainly obtain energy from the oxidation of inorganic substances, and use the energy released by oxidation to produce ATP to assimilate carbon dioxide to produce nutrients needed by synthetic cells. Therefore, such microorganisms grow slowly, have a long generation time, and have a slow value-added speed, and are not easy to be quickly cultivated, and it is difficult to apply them in large scale in industry. Therefore, for autotrophic microorganisms, in addition to studying the amount and method of adding nutrients, a lot of research work has also been carried out on the cultivation conditions such as pH, tempe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q3/00
CPCC12N1/20C12Q3/00
Inventor 王刚高会杰陈明翔孙丹凤
Owner CHINA PETROLEUM & CHEM CORP
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