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Composite micro-ecological bacterial agent for improving eutrophication of water body and preparation method thereof

A compound micro-ecology and water eutrophic technology is applied in the field of compound micro-ecological inoculants for improving water eutrophication and the preparation thereof, so as to achieve the effects of reducing the risk of environmental pollution and reducing excessive enrichment.

Pending Publication Date: 2021-12-03
ANHUI TECH BANK BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of compound micro-ecological bacterial agent that improves water body eutrophication and preparation method thereof, and the compound micro-ecological bacterial agent that reduces water body eutrophication provided by the present invention, each bacterial strain can synergize, reduces water body ammonia nitrogen, reduces Organophosphorus and hydrogen sulfide poisoning, maintain water quality suitable, reduce environmental pollution, to solve the problems raised in the above-mentioned background technology

Method used

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  • Composite micro-ecological bacterial agent for improving eutrophication of water body and preparation method thereof

Examples

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Effect test

Embodiment 1

[0020] A compound micro-ecological bacterial agent for improving eutrophication in water bodies, including Bacillus subtilis, Bacillus megaterium, Bacillus arborii, Thiobacillus and their respective corresponding Bacillus and Thiobacillus carrier culture medium, with stronger stress resistance Bacillus arborii can be used in harsher water conditions. At the same time, the introduction of Thiobacillus thiazobacillus reduces the concentration of hydrogen sulfide in water, and reduces the toxic effect of water pollutants under high-density breeding conditions, so as to reduce ammonia nitrogen and sulfide in aquaculture water. Hydrogen and organic phosphorus, maintaining suitable water quality is the main research and development direction, rationally formulating the production and drying process of different strains, and synthesizing the metabolic characteristics of Bacillus subtilis, Bacillus megaterium, Bacillus arborii and Thiobacillus, so that they can work synergistically to r...

Embodiment 2

[0036] A compound micro-ecological agent for improving eutrophication in water bodies, comprising Bacillus subtilis, Bacillus megaterium, Bacillus arborii, Thiobacillus and their respective corresponding Bacillus and Thiobacillus carrier culture media.

[0037] Further, the Bacillus culture medium is: 2.0 g of peptone, 10.0 g of yeast extract, 2.0 g of dipotassium hydrogen phosphate, 1.0 g of glucose, 15.0 g of agar, adjust the pH to 7.2, and distilled water to a constant volume of 1000 ml.

[0038] Further, the Thiobacillus culture medium is: (NH 4 ) 2 SO 4 0.20g, K 2 HPO 4 3.00g, MgSO 4 ·7H2 O0.40g, CaCl 2 0.20g, FeSO 4 ·7H 2 O 0.01g, S 5.00g, adjust the pH to 2.0, and set the volume to 1000ml.

[0039] The preparation method of the compound micro-ecological agent for improving eutrophication of water bodies is the same as that in Example 1.

Embodiment 3

[0041] A compound micro-ecological agent for improving eutrophication in water bodies, comprising Bacillus subtilis, Bacillus megaterium, Bacillus arborii, Thiobacillus and their respective corresponding Bacillus and Thiobacillus carrier culture media.

[0042] Further, the Bacillus culture medium is: 8.0 g of peptone, 20.0 g of yeast extract, 4.0 g of dipotassium hydrogen phosphate, 3.0 g of glucose, 25.0 g of agar, adjust the pH to 7.2, and distilled water to a constant volume of 1000 ml.

[0043] Further, the Thiobacillus culture medium is: (NH 4 ) 2 SO 4 0.40g, K 2 HPO 4 4.00g, MgSO 4 ·7H 2 O0.60g, CaCl 2 0.30g, FeSO 4 ·7H 2 O 0.01g, S 15.00g, adjust the pH to 2.0, and set the volume to 1000ml.

[0044] The preparation method of the compound micro-ecological agent for improving eutrophication of water bodies is the same as that in Example 1.

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Abstract

The invention discloses a composite micro-ecological bacterial agent for improving eutrophication of a water body and a preparation method thereof, and belongs to the technical field of microbial fermentation. The composite micro-ecological bacterial agent comprises bacillus subtilis, bacillus megatherium, bacillus aryabhattai, thiobacillus and respectively corresponding bacillus and thiobacillus carrier culture media. According to the composite micro-ecological bacterial agent for reducing eutrophication of the water body, which is provided by the invention, the main research and development direction is to reduce ammonia nitrogen, hydrogen sulfide and organophosphorus in the aquaculture water body and maintain proper water quality, different strain production and drying processes are reasonably prepared, and metabolic characteristics of the bacillus subtilis, the bacillus megatherium, the bacillus aryabhattai and the thiobacillus are integrated, so that the bacillus subtilis, the bacillus megatherium, the bacillus aryabhattai and the thiobacillus can take a synergistic effect, the aims of reducing excessive enrichment of ammonia, nitrogen, sulfur and phosphorus and reducing the risk of environmental pollution are fulfilled, the proper water quality is maintained, and the environmental pollution is reduced.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a compound micro-ecological bacterial agent for improving eutrophication in water bodies and a preparation method thereof. Background technique [0002] In aquaculture, especially in the cultivation of special aquatic products, due to the continuous increase in breeding density, the residues of ammonia nitrogen, sulfur deposition caused by feed residues and metabolites of aquatic products, and the residues of drugs such as water disinfectants used for disease resistance have also increased significantly. As a result, the load on the aquaculture water body has also increased, causing a series of water quality risks such as discoloration, odor, and algal blooms, and seriously affecting the yield and aquatic life safety. Therefore, a compound microecological bacterial agent that can reduce water pollution and improve water eutrophication is currently actual needs. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/11C12R1/07C12R1/01C02F101/10C02F101/16C02F101/30C02F103/20
CPCC12N1/20C02F3/34C02F2101/16C02F2101/101C02F2101/30C02F2103/20
Inventor 胡传炯朱颍琨李姗周小波徐杰陈桂萍
Owner ANHUI TECH BANK BIO TECH
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