Acidophilus thiobacillus and its method for treating mine acid wastewater and recovering iron resources

A technology of mine acid waste water and acid thiobacillus, applied in the field of microorganisms, can solve the problems of large amount of slag, secondary pollution, inability to recover waste water iron and other valuable metals, etc.

Active Publication Date: 2020-10-30
有研资源环境技术研究院(北京)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the conventional treatment method is lime neutralization method, which produces a large amount of slag, cannot recover iron and other valuable metals in wastewater, and is prone to secondary pollution

Method used

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  • Acidophilus thiobacillus and its method for treating mine acid wastewater and recovering iron resources

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Acquisition and Identification of Acidophilus Thiobacillus

[0033] The obtaining method of acidic thiobacillus acidophilus provided by the invention is:

[0034] 1) Add 0.3g of yeast powder to 100mL of the water sample containing the acidic thiobacillus acidophilus, enrich and culture at 30°C with gentle shaking at 100rpm for 1 week, and use a microscope to detect the growth of the bacteria; the water sample is taken from Jiangxi De Xing copper mine copper-containing acid wastewater. Bacteria grow to measure the absorbance value of the bacterial culture solution at 600nm, if the obtained OD600 value is between 0.6-0.8.

[0035] 2) Filter the bacteria in the above culture solution by membrane filtration, and wash the bacteria with 20 mL of sterile water. Inoculate multiple 100mL enrichment media with 5% inoculum amount, culture at 45°C, and set no inoculation control (CK). The growth of bacteria was observed after two weeks of culture on a shaker at 100rpm....

Embodiment 2

[0039] Example 2 Treatment of Mine Acidic Wastewater and Recovery of Iron Resources

[0040] 1) The acid wastewater from the Dexing Copper Mine in Jiangxi is passed into a biological oxidation reactor, and the biological oxidation reactor is filled with commercially available soft packing for water treatment.

[0041] The wastewater quality is as follows: pH2.3; Cu 2+ : 220mg / L, total iron ion concentration: 1480mg / L.

[0042] 2) Inoculate the identified bacterial species of Acidophilus thiobacillus into the enrichment medium, and culture it on a shaker at 45°C at 100rpm until the bacterial concentration is 10 8 each / mL; then inoculate the bacterial solution into the biooxidation reactor with an inoculum volume of 3L / m 3 .

[0043] The biooxidation reactor used is a conventional packed biofilm reactor with air aeration at the bottom, and the treatment time is 1 hour.

[0044] 3) After the reaction, the effluent was passed into the solid-liquid separation reactor, the pH wa...

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Abstract

The invention provides a thiobacillus acidophilus, the name of the strain is: Thiobacillus ferrooxidans ST-A, the depository unit is China National Center for Typical Culture Collection, address: Wuhan University, deposit date: May 24, 2015, deposit registration number : CCTCC NO: M2015333. Using this bacteria to treat iron-containing mine acid wastewater can accelerate the oxidation of ferrous ions to generate ferric ions at low pH; at a suitable pH, the bacteria can catalyze ferric ions to form Shi's minerals (Fe 8 o 8 (OH) 6 (SO 4 )) Precipitation, effective recovery of iron resources, and the recovered iron-containing precipitates will not entrain other valuable metals (copper, zinc, etc.), providing an operational process for the recovery of metal resources in mine acid wastewater.

Description

technical field [0001] The invention relates to the technical field of microorganisms. In particular, it relates to a bacterium of the genus Thiobacillus, its cultivation method and the process of using the bacterium to treat acid waste water from iron-containing mines and recover iron resources. Background technique [0002] Sulfide mines all have the problem of mine acid wastewater. As long as the pyrite is exposed to the air or the groundwater containing dissolved oxygen contacts the ore, the ore will slowly oxidize to produce ferrous ions. At this time, due to the high pH, ​​ferrous ions can be rapidly oxidized into ferric ions, and the presence of ferric ions will accelerate the oxidation process of pyrite and cause the pH to drop rapidly. When the pH further drops to 3.5 Next, under the catalysis of microorganisms, ferric ions will be continuously produced, which will further increase the oxidation rate of pyrite and other sulfide ores, and accelerate the production o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12P3/00C12R1/01C02F103/10C02F101/20
CPCC02F3/346C02F2101/203C02F2103/10C12N1/20C12P3/00C12N1/205C12R2001/01
Inventor 刘兴宇刘文彦陈勃伟张明江温建康
Owner 有研资源环境技术研究院(北京)有限公司
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