SPR detection device and SPR detection method using optical dispersion

Abstract

The invention discloses an SPR detection device using optical dispersion. The SPR detection device comprises a wide-spectrum light source, a prism and a detection component, wherein an incident side component is arranged between the wide-spectrum light source and the prism, a correction component is arranged between the prism and the detection component, and a detection chip is arranged on the reflective bottom surface of the prism; the detection chip comprises a gold film and a flow cell, a channel is arranged on one side surface of the flow cell facing the gold film, the gold film covers theflow cell, and the gold film is positioned between the flow cell and the reflective bottom surface of the prism; and the detection component comprises a dispersion prism and a detector, wherein the dispersion prism is used for dispersing the reflected light corrected by the correction component, and the detector is used for recording the spectrum of the reflected light dispersed by the dispersionprism. The invention also discloses a multi-channel SPR detection method using optical dispersion. According to the SPR detection device and the SPR detection method using optical dispersion disclosed by the invention, the wide-spectrum light source is used to perform one-time imaging detection on the complex refractive index of a metal film, so that the requirements of multi-channel detection can be met.

Description

technical field [0001] The invention belongs to the technical field of biological monitoring, in particular to an SPR detection device and an SPR detection method using light dispersion. Background technique [0002] The Surface Plasmon Resonance (SPR, Surface Plasmon Resonance) sensor has been developed for 40 years. It is mainly used for the detection of biological macromolecules. It shows the advantages of no labeling, real-time detection, and a small amount of samples. A research hotspot in the field of sensors. Most of the mature technologies are now in the hands of foreigners, but most of the existing commercialized instruments are bulky and expensive, so a cost-effective miniaturized, low-cost portable SPR instrument is studied to improve product quality. practicability to meet the clinical, field and other use in a variety of occasions. [0003] Modulation in most devices today is based on: angle, wavelength, intensity, and phase. The feature points of the intensi...

AI Technical Summary

Problems solved by technology

However, since the SPR detection system uses an area array detector to take two-dimensional images for local scanning, it cannot meet the requirements of multi-channel detection. In addition, the resonance wavelength corresponding to the sensing position is obtained through image pixels, and its detection accuracy is relatively low. Low

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