The invention relates to a hyperspectral
microscopic imaging system which comprises a front imaging module and a hyperspectral rear imaging module. The front imaging module is used for collecting a sample optical
signal and imaging to obtain a front image
light beam; the hyperspectral rear imaging module is used for carrying out dispersion on the front image
light beam according to the dispersion direction to obtain a spatial
spectral image; comprising a magnifying lens group, a segmentation
assembly and a dispersion lens group, the front image
light beam passes through the magnifying lens group and is magnified into a magnified image in the dispersion direction, and the segmentation
assembly is arranged on the
image plane of the magnified image; the segmentation
assembly comprises a plurality of micro-lens groups which are arranged side by side in the dispersion direction and cover the range of the amplified image; the dispersion lens group comprises a collimating lens and a converging lens which have the same
focal length, the collimating lens and the converging lens form a
relay lens group, and a dispersion
prism is arranged between the collimating lens and the converging lens according to the dispersion direction. According to the hyperspectral
microscopic imaging system provided by the invention, the multispectral superposed space image obtained by the front imaging module is amplified through the amplification lens group, then is segmented by the segmentation assembly and is converged into discrete sub-images, camera detection resources for recording spectral information are created, the dissociated spectral information is integrated into the space information, and the
imaging quality is improved. And the image is recorded by the camera to obtain a space-spectrum image without superposition
crosstalk, so that the
distortion of space information is reduced while space-spectrum separation is realized.