A device to illuminate a object, to excite its
fluorescence light emission, and detect the emitted
fluorescence spectrum, comprising: at least one illumination
system (13), adapted to receive light from a
light source (11), to select at least one
wavelength bands of
light spectrum of the source (11), to illuminate a object (15) with light filtered in that way (14); and a detection
system (17), adapted to detect
fluorescence light (16) emitted by the object (15), to select at least one
wavelength bands of fluorescence,
light spectrum (16), to
record the spectrum of the filtered light; characterized in that said illumination
system (13) comprises: at least one first dispersive element (41), at least one focusing
optics (43), at least one spatial fitter of excitation (44), at least one collimating
optics (45) and at least one second dispersive element (47), wherein said detection system (17) comprises: at least one dispersive element (81), at least one focusing
optics (83), at least one
spatial filter of detection (84), at least one imaging optics (85) and at least one
light detector (87).