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Transplanting method of wooly savatier monochasma herb

A velvet antler grass and a transplanted technology are applied in the field of transplanting velvet velvet grass, which can solve the problems that artificial cultivation cannot be fully realized, the development of velvet velvet grass is limited, and there is no breakthrough in the open-air cultivation of test-tube seedlings. The effect of transplanting survival rate, solving bottleneck problems and enhancing adaptability

Pending Publication Date: 2019-05-17
浙江省中药研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture test-tube plantlets and seed-sterilized test-tube plantlets of velvet velvet have matured, but the open-air cultivation of test-tube plantlets has not yet broken through, artificial cultivation cannot be fully realized, and the development of related industries of velvet velvet is limited

Method used

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  • Transplanting method of wooly savatier monochasma herb
  • Transplanting method of wooly savatier monochasma herb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Two months after germination, the test-tube seedlings that were tissue-cultured with velvet grass seeds were transplanted and cultured. The medium was 1 / 2MS, pH=7, agar 5g / L, activated carbon 1g / L, and the tissue cultured seedlings had 2 Cotyledons, 2-4 true leaves with white hairs, 3-4 roots over 2cm long.

[0035] Prepare a solution of 0.01% fludioxonil with sterile water, sterilize under high temperature and high pressure at 121°C for 15 minutes to obtain a sterilizing solution, pour the sterilizing solution into the culture bottle of the above-mentioned test tube seedlings, and open the upper cover to cultivate in the tissue culture room for 7 days, keep the liquid The surface height is below 0.3cm.

[0036] After hardening, the seedlings are transplanted in the greenhouse to grow seedlings, prepare the culture medium of leaf humus, peat soil, perlite, and broken sphagnum moss=2.5:2:1:0.5 (volume ratio), and sterilize under high pressure at 121 degrees Celsius for 1...

Embodiment 2

[0040] Three months after germination, the test-tube seedlings that were tissue-cultured with the seeds of velvet grass were transplanted and cultured. The medium was 1 / 2MS, pH=7, agar 5g / L, activated carbon 1g / L, and the tissue cultured seedlings had 2 Cotyledons, 2-6 true leaves with white hairs, 3-4 roots over 2cm long.

[0041]Prepare a solution of 0.01% fludioxonil with sterile water, sterilize under high temperature and high pressure at 121°C for 15 minutes to obtain a sterilizing solution, pour the sterilizing solution into the culture bottle of the above-mentioned test tube seedlings, and open the upper cover to cultivate in the tissue culture room for 7 days, keep the liquid The surface height is below 0.3cm.

[0042] After hardening, the seedlings are transplanted in the greenhouse to grow seedlings, prepare the culture medium of humus, peat soil, perlite, and broken sphagnum moss=3.5:2:1:0.5 (volume ratio), and sterilize under high pressure at 121 degrees Celsius fo...

Embodiment 3

[0047] Three months after germination, the test-tube seedlings that were tissue-cultured with the seeds of velvet grass were transplanted and cultured. The medium was 1 / 2MS, pH=7, agar 5g / L, activated carbon 1g / L, and the tissue cultured seedlings had 2 Cotyledons, 2-6 true leaves with white hairs, 3-4 roots over 2cm long.

[0048] Prepare a solution of 0.01% fludioxonil with sterile water, sterilize under high temperature and high pressure at 121°C for 15 minutes to obtain a sterilizing solution, pour the sterilizing solution into the culture bottle of the above-mentioned test tube seedlings, and open the upper cover to cultivate in the tissue culture room for 7 days, keep the liquid The surface height is below 0.3cm.

[0049] The seedlings after hardening are transplanted in the greenhouse to grow seedlings, prepare the medium of leaf humus, peat soil, perlite, broken sphagnum moss=3:2:1:0.5 (volume ratio), and sterilize by autoclaving at 121 degrees Celsius for 15 minutes t...

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Abstract

The invention relates to a transplanting method of wooly savatier monochasma herb and belongs to the technical field of artificial cultivation and production of wooly savatier monochasma herb. The transplanting method comprises the following steps: domesticating wooly savatier monochasma herb test-tube seedlings with 2 to 4 months after germination to obtain domesticated seedlings; transplanting the domesticated seedlings into a nursing substrate and growing seedlings; after transplanting, controlling humidity and illumination intensity; and finally big field transplanting. By the transplanting method, the adaptability of the test-tube seedlings on the open environment can be enhanced and the transplanting survival rate of the cultivated wooly savatier monochasma herb test-tube seedlings is increased.

Description

technical field [0001] The invention relates to the technical field of artificial cultivation and production of velvet velvet, in particular to a method for transplanting velvet velvet. Background technique [0002] Scrophulariaceae velvet grass is a perennial herb of the genus Scrophulariaceae velvet velvet. It grows in sunny mountains and grasses, and has the functions of clearing away heat and detoxification, cooling blood and stopping bleeding. The tissue culture test-tube plantlets and seed-sterilized test-tube plantlets of velvet velvet have matured, but the open-air cultivation of test-tube plantlets has not yet broken through, artificial cultivation cannot be fully realized, and the development of related industries of velvet velvet is limited. Contents of the invention [0003] The object of the present invention is to provide a method for transplanting pilose antler grass. The transplanting method of the invention can enhance the adaptability of the test-tube se...

Claims

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Application Information

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IPC IPC(8): A01G20/00A01G24/15A01G24/28
Inventor 孙健沈晓霞王志安
Owner 浙江省中药研究所有限公司
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