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A method for determining the content of spray for tendon and bone injury

A measurement method and spray technology, applied in the field of medical inventions, can solve the problems of complicated operation, low efficiency, and too simple, etc., and achieve the effects of high detection accuracy, stable detection baseline, and good stability

Active Publication Date: 2022-03-22
贵州远程制药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Muscle and Bone Injury Spray is a traditional Chinese medicine compound preparation made by extracting Chishan Powder, Radix Paeoniae Rubra, Epimedium, Earthworm, Aconitum Aconitum, and Menthol. It has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, and is used for soft tissue Injury, the active ingredients in the tendon and bone injury spray are: paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, baohuoside I, etc., but the tendon and bone injury spray There is no scientific detection method for these key active ingredients, so that it can achieve a more comprehensive quality detection
[0003] The quantitative index in the current quality standard of Jingushang Spray is paeoniflorin in Radix Paeoniae Rubra, which is not used much in prescriptions. For Chishan Powder, in the quality standard, it is only identified by TLC , this standard is too simple to achieve effective control of product quality
[0004] Existing literature Xu Chaofei, Quality research and quality standard improvement of Miao medicine "Jingushang Spray" [D], Guiyang Medical College, 2015, disclosed a simultaneous determination of 4 components 3,3' in Jingushang Spray by HPLC -Dimethylellagic acid, 3,3',4'-trimethylellagic acid, paeoniflorin, and icariin. This method simultaneously determines the content of four components in a traditional Chinese medicine preparation, but There are many components that affect the quality of tendon and bone injuries. Only measuring 4 of them cannot effectively detect the active ingredients in the product, and methylellagic acid accounts for 2 of them. Experiments have shown that there are many active ingredients in Epimedium, and only measuring Obviously, icariin cannot effectively detect the active ingredients in Epimedium, so this method cannot scientifically determine the content of active ingredients in the tendon and bone injury spray. Determination of reference substance, complicated operation and low efficiency

Method used

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  • A method for determining the content of spray for tendon and bone injury
  • A method for determining the content of spray for tendon and bone injury
  • A method for determining the content of spray for tendon and bone injury

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The content of the active ingredients paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, and baohuoside I in Jingushang Spray was detected according to the following method.

[0033] Chromatographic conditions: Agilent ZORBA*S8~C18 column; the inner diameter is 4.6*150mm, 5um, the mobile phase B is 0.05mol / L potassium dihydrogen phosphate solution, the mobile phase C is methanol, the mobile phase D is acetonitrile, and the detection wavelength is 0 ~23min: 230nm, 23~54min: 254nm, 54~137min: 270nm, column temperature 35℃, gradient elution method:

[0034] time mobile phase B mobile phase C mobile phaseD flow rate 0~14min 92％～90％ —— 8％～10％ 1ml / min 14~17.5min 90％ —— 10％ 1ml / min 17.5~22min 90％～88％ —— 10％～12％ 1ml / min 22~33min 88％～83％ —— 12％～17％ 1ml / min 33~50min 83％～79％ —— 17％～21％ 1ml / min 50~51min 79％～77.5％ —— 21％～22.5％ 1~0.85ml / min 51~80min 77.5％ —— 22.5％ 0.85ml / min ...

Embodiment 2

[0042] The content of the active ingredients paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, and baohuoside I in Jingushang Spray was detected according to the following method.

[0043] Chromatographic conditions: Agilent ZORBA*S8~C18 column; the inner diameter is 4.6*150mm, 5um, the mobile phase B is 0.05mol / L potassium dihydrogen phosphate solution, the mobile phase C is methanol, the mobile phase D is acetonitrile, and the detection wavelength is 0 ~23min: 230nm, 23~54min: 254nm, 54~137min: 270nm, column temperature 35℃, gradient elution method:

[0044]

[0045]

[0046] Preparation of the test solution: Take 10ml of tendon and bone injury spray, accurately absorb, put it in a 100ml evaporating dish, evaporate to dryness, add 5ml of aqueous solution to dissolve, transfer the solution to a 25ml test tube, add 10ml of water-saturated n-butanol solution in the test tube , extract, add 10ml of water-saturated n-butanol solution for extraction, t...

Embodiment 3

[0051] The content of the active ingredients paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, and baohuoside I in Jingushang Spray was detected according to the following method.

[0052] Chromatographic conditions: Agilent ZORBA*S8~C18 column; the inner diameter is 4.6*150mm, 5um, the mobile phase B is 0.05mol / L potassium dihydrogen phosphate solution, the mobile phase C is methanol, the mobile phase D is acetonitrile, and the detection wavelength is 0 ~23min: 230nm, 23~54min: 254nm, 54~137min: 270nm, column temperature 35℃, gradient elution method is as follows:

[0053] time mobile phase B mobile phase C mobile phaseD flow rate 0~14min 92％～90％ —— 8％～10％ 1ml / min 14~17.5min 90％ —— 10％ 1ml / min 17.5~22min 90％～88％ —— 10％～12％ 1ml / min 22~33min 88％～83％ —— 12％～17％ 1ml / min 33~50min 83％～79％ —— 17％～21％ 1ml / min 50~51min 79％～77.5％ —— 21％～22.5％ 1~0.85ml / min 51~80min 77.5％ —— 22.5％ ...

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Abstract

The invention relates to a content determination method for a spray for tendon and bone injury, in particular to effective ingredients paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B and epimedin in the tendon and bone injury spray The content assay method of 7 kinds of compositions such as C, baohuoside I, comprises the preparation of need testing solution, the preparation of reference substance solution, HPLC method mensuration, adopts the content assay method provided by the invention, can measure multiple active ingredients simultaneously, The detection line is stable, the separation is good, the detection accuracy is high, and the stability is good, the content determination time is shortened, the detection efficiency is improved, and a relatively comprehensive quantitative detection method is provided for controlling the quality of the bone injury spray. The study on the stability of the tendons and bones injury spray provides a more comprehensive method for the quantitative study of the stability of the active ingredients.

Description

technical field [0001] The invention relates to the field of medical inventions, in particular to a method for determining the content of a spray for tendons and bones. Background technique [0002] Muscle and Bone Injury Spray is a traditional Chinese medicine compound preparation made by extracting Chishan Powder, Radix Paeoniae Rubra, Epimedium, Earthworm, Aconitum Aconitum, and Menthol. It has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, and is used for soft tissue Injury, the active ingredients in the tendon and bone injury spray are: paeoniflorin, icariin, ellagic acid, epimedin A, epimedin B, epimedin C, baohuoside I, etc., but the tendon and bone injury spray There is no scientific detection method for these key active ingredients, so that it can achieve a more comprehensive quality detection. [0003] The quantitative index in the current quality standard of Jingushang Spray is paeoniflorin in Radix Paeoni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 伍庆程吉祥刘青唐勇薛静罗天军伍朝平王娇李佳蔚
Owner 贵州远程制药有限责任公司
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