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Cell or Particle Counting Method Based on Surface Acoustic Wave Technology

A surface acoustic wave and particle counting technology, which is applied in the field of cell counting, can solve problems such as high operating environment requirements, difficult popularization in remote areas, and complicated operation, and achieve real-time test data, avoid manual operation, and improve technical accuracy.

Active Publication Date: 2022-05-24
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has fast detection speed and high detection accuracy, the supporting equipment is expensive, the operation is complicated, the detection cost is high, and the operating environment requirements are high. It is difficult to popularize in developing countries or remote areas, and its function is not only for cells. count a case
This will be somewhat wasteful for detection experiments that only have specific requirements
[0006] Image cytometer technology: a new type of cell detection technology combined with microscope and flow cytometry, combining optical precision instrument measurement of cell morphological parameters with image processing technology, not only can measure a large number of cells, but also can study the morphology of specific cells, and obtain cell follow-up Time-varying information and morphological characteristics of cells inside the tissue, but this imaging method takes a long time and cannot obtain the desired results in real time
[0007] In view of the above-mentioned disclosed cell counting method, there are several defects. Although red blood cell counting can quickly obtain the result, the result may be high, and it is only suitable for large-volume cell counting; flow cytometry has fast detection speed and high accuracy. However, the price is expensive, and it is difficult to popularize in remote areas; the imaging time of image cytometer is too long, and the results cannot be obtained in real time

Method used

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  • Cell or Particle Counting Method Based on Surface Acoustic Wave Technology
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  • Cell or Particle Counting Method Based on Surface Acoustic Wave Technology

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Embodiment Construction

[0037] The present invention will be further described below in conjunction with the accompanying drawings.

[0038] The cell counting method based on surface acoustic wave technology is as follows:

[0039] 1) Fabrication of a surface acoustic wave device with interdigital electrodes on a piezoelectric substrate.

[0040] 2) A microfluidic channel is fabricated on the surface acoustic wave propagation path of the surface acoustic wave device.

[0041] 3) The cell fluid (mixture of culture medium and cells) is transported into the microfluidic channel through a micropump connected to a syringe, and the cell fluid is driven by the sheath flow technology to achieve focusing, so that the cells in the microfluidic channel present a single arrangement, and the cells maintain a distance. through the microfluidic channel.

[0042] 4) The surface acoustic wave device is connected with the detection instrument. After the detection instrument works, the transmission parameter is measu...

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Abstract

The invention discloses a cell or particle counting method based on surface acoustic wave technology. Existing cell counting methods are either only suitable for large volume cell counts or are expensive or take too long. The present invention makes a microfluidic channel on the surface acoustic wave propagation path of a surface acoustic wave device; connects a syringe with a micropump to transport cell fluid or particle fluid into the microfluidic channel, uses sheath flow technology to drive the cell fluid or particle fluid to achieve focusing, and makes the microfluidic The cells or particles in the flow channel are in a single arrangement, and the distance between the cells or particles is kept, and they flow through the micro-flow channel in turn; the surface acoustic wave device is connected to the detection instrument, and after the detection instrument works, the transmission parameters are measured. By drawing the transmission parameters at the resonance frequency An image of point values ​​over time to obtain cell or particle population information. The present invention realizes by testing transmission parameters, achieves real-time test data, and improves technical precision.

Description

technical field [0001] The invention relates to cell counting technology, in particular to a cell or particle counting method based on surface acoustic wave technology. Background technique [0002] Cells are the most basic units that constitute the structure and function of living organisms and perform various functions of living organisms. Every day in the human body, many cells (RBCs) age and die and many cells are regenerated to maintain the dynamic balance of the metabolism of the living body. But if this homeostasis is disrupted, it will cause a series of health problems. By detecting the number of specific cells, certain diseases can be identified and diagnosed, so cell counting is of great significance to health care, disease treatment, and medical research, and it is also a very useful tool. For example, CD+4 lymphocytes in HIV-infected people will gradually decrease, and detection of the number of CD+4 lymphocytes can help diagnose the disease; red blood cells in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/10
CPCG01N15/10G01N2015/1006
Inventor 轩伟鹏胡天玉张文瑞陈金凯黄汐威孙玲玲骆季奎
Owner HANGZHOU DIANZI UNIV
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