Cell or Particle Counting Method Based on Surface Acoustic Wave Technology
A surface acoustic wave and particle counting technology, which is applied in the field of cell counting, can solve problems such as high operating environment requirements, difficult popularization in remote areas, and complicated operation, and achieve real-time test data, avoid manual operation, and improve technical accuracy.
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[0037] The present invention will be further described below in conjunction with the accompanying drawings.
[0038] The cell counting method based on surface acoustic wave technology is as follows:
[0039] 1) Fabrication of a surface acoustic wave device with interdigital electrodes on a piezoelectric substrate.
[0040] 2) A microfluidic channel is fabricated on the surface acoustic wave propagation path of the surface acoustic wave device.
[0041] 3) The cell fluid (mixture of culture medium and cells) is transported into the microfluidic channel through a micropump connected to a syringe, and the cell fluid is driven by the sheath flow technology to achieve focusing, so that the cells in the microfluidic channel present a single arrangement, and the cells maintain a distance. through the microfluidic channel.
[0042] 4) The surface acoustic wave device is connected with the detection instrument. After the detection instrument works, the transmission parameter is measu...
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