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Quantitative protein silver staining method of freshwater ciliates

A protein silver and ciliate technology, which is applied in the field of quantitative protein silver dyeing method, can solve the problems of long time-consuming production, uneven dyeing of filter membrane, poor dyeing effect of small ciliates, etc., and achieves good dyeing effect.

Inactive Publication Date: 2019-11-12
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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  • Description
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Problems solved by technology

However, this method still has disadvantages such as poor staining effect of small ciliates such as oligochaetes, long average preparation time for a single sample, uneven staining of the filter membrane, hard and brittle and difficult to seal, etc.

Method used

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  • Quantitative protein silver staining method of freshwater ciliates
  • Quantitative protein silver staining method of freshwater ciliates
  • Quantitative protein silver staining method of freshwater ciliates

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Embodiment Construction

[0035] In order to make the implementation technical means, creative features, objectives and effects of the present invention easy to understand, the present invention will be further elaborated below in conjunction with specific illustrations.

[0036] Such as Figure 1~3 as shown,

[0037] In the present embodiment, a quantitative protein silver staining method of freshwater ciliates comprises the following steps:

[0038] Step 1: Sample fixation: Fix the original water sample with color fixative Bouin's solution, the final concentration is 5% (v / v);

[0039] Step 2: Filtration and concentration: take an appropriate amount of the sample in step 1 and place it in a filter covered with nitrocellulose membrane for suction filtration;

[0040] Step 3: Agar embedding: including the following sub-steps;

[0041] (1) Take out the filter membrane loaded with worms and dry on the surface in step 2 and place it in the center of a continuously insulated glass slide, take another ho...

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Abstract

The invention provides a quantitative protein silver staining method of freshwater ciliates. The method includes sample fixation, filtration and concentration, agar embedding, agar solidification, protein silver staining and dehydrating and mounting. According to the method, a staining effect is better, slices produced in unit time are more, an overall staining effect is uniform, and mounting is easy. The method is more suitable for protein silver staining technology of qualitative and quantitative study of planktonic ciliates in typical eutrophic lakes of China.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a quantitative protein silver staining method for freshwater ciliates. Background technique [0002] Ciliates are unicellular microscopic organisms, most of which live in planktonic freshwater with a body length of 10-200 μm. Because of its small size, it is difficult to operate, and many taxonomic features cannot be seen directly, so the identification of species is more difficult, and many species have varying degrees of confusion and errors. [0003] Direct biopsy is the most conservative method for identifying ciliates. However, microscopic examination of living organisms needs to be completed within a few hours after sampling. If the transportation and storage time is too long, some sensitive species will disappear in large numbers as the temperature and chemical properties of water samples change. Furthermore, encapsulation, de-encapsulation and predation will change the init...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/31
CPCG01N1/30G01N1/31
Inventor 李静陈非洲卢文轩杨坤方婷梁阳阳赵秀侠
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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