Application of cytotoxic anti-tumor drugs to preparation of tumor-related tissues

An anti-tumor drug and tumor-related technology, applied in the application field of cytotoxic anti-tumor drugs in the preparation of tumor-related tissues, can solve problems such as limitations, and achieve the effect of promoting tumor growth or tumor cell proliferation

Inactive Publication Date: 2020-01-17
THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the preparation of tumor-related models, tissues, cells and related components is currently limited.

Method used

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  • Application of cytotoxic anti-tumor drugs to preparation of tumor-related tissues
  • Application of cytotoxic anti-tumor drugs to preparation of tumor-related tissues
  • Application of cytotoxic anti-tumor drugs to preparation of tumor-related tissues

Examples

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preparation example Construction

[0039] The preparation of the tumor-related tissue includes in vitro or in vitro preparation methods or processes, and the scope of preparation includes but not limited to in vivo tumor-bearing animals or tumor tissues, isolated tumor tissues or tumor tissue components, in vitro tumor cells, tumor stem cells Or tumor microenvironment tissue cells, and use the above drugs and tumor tissue, tumor cells, tumor stem cells or tumor microenvironment cells to obtain various cytokines or compound products. Tumor microenvironment tissue cells include but are not limited to fibroblasts, pericytes, endothelial cells, immune cells, and bone marrow-derived cells in tumor tissues.

[0040] The present invention studies the influence of cytotoxic antineoplastic drugs on tumor growth under single or multiple dose administration conditions. Specific examples are as follows.

Embodiment 1

[0042] Effects of Low-dose Cytotoxic Antineoplastic Drugs on the Growth of Sarcoma Tumor in S180 Mice

[0043] (1) Effect of low-dose cyclophosphamide (CTX) on the growth of S180 tumors

[0044] Kunming mice subcutaneously inoculated with S180 tumors were randomly divided into 6 groups: control group, 10mg / kg CTX group, 20mg / kg CTX group, 40mg / kg CTX group, 60mg / kg CTX group and 80mg / kg CTX group. CTX low-dose intraperitoneal administration multiple times, the drug showed a two-way effect on the growth of S180 tumors: at a lower dose, the tumor growth increased with the increase of the drug dose, showing a significant promoting effect; when the dose of cyclophosphamide reached a certain When the effective dose is increased, it also exhibits the effect of inhibiting tumor growth. (like figure 1 shown)

[0045] (2) Effect of low-dose methotrexate (MTX) on the growth of S180 tumor

[0046] Kunming mice subcutaneously inoculated with S180 tumors were randomly divided into 5 gr...

Embodiment 2

[0054] Effects of Low-Dose Cytotoxic Antineoplastic Drugs on the Growth of Melanoma Tumor in B16 Mice

[0055] (1) Effect of low-dose cyclophosphamide (CTX) on the growth of B16 tumors

[0056] Thirty-six C57BL / 6 mice subcutaneously inoculated with B16 were randomly divided into 6 groups: control group, 2.5mg / kg CTX group, 5mg / kg CTX group, 10mg / kg CTX group, 20mg / kg CTX group and 40mg / kg CTX group Group, intraperitoneal multiple administration of low dose intervals. The results showed that the low-dose interval of CTX also had a bidirectional effect on the growth of B16 tumors: at lower doses, the tumor growth increased with the increase of the drug dose, and the drug showed a significant promoting effect on tumor growth; when cyclophosphamide When the dose reaches a certain dose, it will show the effect of inhibiting tumor growth. (like Image 6 shown)

[0057] (2) Effect of low-dose methotrexate (MTX) on B16 tumor growth

[0058] Thirty female C57BL / 6 mice inoculated w...

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Abstract

The invention provides application of cytotoxic anti-tumor drugs to preparation of tumor-related tissues. A research proves that under a drug administration condition of certain concentration or dosage being less than conventional maximum tolerance dose, a result proves that the drugs have an effect of obviously promoting tumor growth; and by utilizing the phenomenon, the drugs can be applied to building of a tumor growth model, production culturing of in vitro and in vivo tumor tissues and tumor cells, culturing of tissue cells closely related with tumor tissue growth in a tumor micro-environment, and separation and extraction of specific tissue components.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of cytotoxic antitumor drugs in preparing tumor-related tissues. Background technique [0002] Malignant tumor is one of the most important diseases leading to human death. Tumor tissue is heterogeneous and consists of tumor cells, tumor stem cells, fibroblasts, endothelial cells, pericytes, lymphocytes, macrophages, and other types of bone marrow-derived cells and local tissue-derived cells. They interact and influence each other through direct effects or indirect pathways such as secreting cytokines, which jointly constitute the tumor microenvironment. In the tumor microenvironment, there are not only cells, cytokines and compound components that can promote tumor growth, but also cells, cytokines and compound components that inhibit tumor growth. The complex heterogeneity of tumor tissue is one of the important reasons why tumors are difficult to cure. However, some ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P35/00A61K31/675A61K31/513A61K31/519A61K33/243
CPCA61K45/00A61P35/00A61K31/675A61K31/513A61K31/519A61K33/243
Inventor 封卫毅赵培培马文兵刘炫鳞唐凤如李厚丽陈敬国赵雪龙欣程凯陈延绅王政朱玉蓉
Owner THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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