A kind of control method of nucleic acid amplification instrument

Abstract

The invention provides a method for controlling a nucleic acid amplification instrument, which includes: step 1, injecting nucleic acid into a first reaction chamber; step 2, operating a heating device, so that the nucleic acid in the first reaction chamber is thermally split, The first control valve is opened, so that the split nucleic acid enters the second reaction chamber through the diversion pipe; step 3, the output control valve is opened, so that the intermediate in the output storage bin flows into the second reaction chamber; Step 4, the controller controls the cooling device to start working to cool down the second reaction chamber; Step 5, the heating device in the second reaction chamber starts to work, the controller controls the second control valve to open, At the same time, the controller also controls the operation of the pump body to transport the nucleic acid in the second reaction chamber back to the first reaction chamber; step 6, setting the steps 1 to 5 as a cyclic reaction , repeat this cycle reaction 20‑30 times.

Description

technical field [0001] The present invention generally relates to a nucleic acid amplification instrument, and more particularly relates to a method for regulating a nucleic acid amplification instrument. Background technique [0002] Nucleic acid amplification is a general term for a large class of technical methods, currently including conventional PCR, real-time fluorescent PCR, isothermal nucleic acid amplification technology, etc. Nucleic acid amplification is very useful in molecular biology and is widely used in biology, therapeutics, diagnostics, forensic And every aspect of the study has broad applicability. Typically, one or more primers are used to generate an amplicon from a starting template, wherein the amplicon corresponds to or is complementary to the template from which the amplicon was generated. Multiplex amplification also simplifies the process and reduces costs. [0003] The existing nucleic acid amplification technology mainly includes: heating to un...

AI Technical Summary

Problems solved by technology

[0004] The existing nucleic acid amplification instrument is not highly intelligent. During the nucleic acid amplification process, the addition of primers, buffers, dNTP and other components requires manual addition by staff;

[0005] At the same time, in the nucleic acid amplification process, it is often necessary to amplify the nucleic acid multiple times, and each nucleic acid amplification process requires temperature control, and these operations need to be performed manually, which is time-consuming and laborious, which is not conducive to production and research; For this reason, the present invention provides a kind of regulation method of nucleic acid amplification instrument, to at least partly solve the above-mentioned problem

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