Regulation and control method of digital nucleic acid amplifier

Abstract

The invention provides a regulation and control method of a digital nucleic acid amplifier. The regulation and control method of the digital nucleic acid amplifier comprises the following steps of (1)injecting nucleic acid into a first reaction chamber; (2) running a heating device, enabling the nucleic acid in the first reaction chamber to be heated and divided, opening a first control valve, and enabling the divided nucleic acid to enter a second reaction chamber through a flow guiding pipeline; (3) opening an output medium control valve, and enabling an intermedium in an output medium storage cabin to flow into the second reaction chamber; (4) using a controller for controlling a heat radiator to start to work so as to cool the second reaction chamber; (5) enabling a heating device inthe second reaction chamber to start to work, using the controller for controlling a second control valve to open, meanwhile, using the controller for controlling a pump body to work, and conveying nucleic acid in the second reaction chamber back into the first reaction chamber; and (6) setting the step 1 to step 5 as circular reaction, and repeating the circular reaction for 20 to 30 times.

Description

technical field [0001] The present invention generally relates to a nucleic acid amplification instrument, and more particularly relates to a control method of a digital nucleic acid amplification instrument. Background technique [0002] Nucleic acid amplification is a general term for a large class of technical methods, currently including conventional PCR, real-time fluorescent PCR, isothermal nucleic acid amplification technology, etc. Nucleic acid amplification is very useful in molecular biology and is widely used in biology, therapeutics, diagnostics, forensic And every aspect of the study has broad applicability. Typically, one or more primers are used to generate an amplicon from a starting template, wherein the amplicon corresponds to or is complementary to the template from which the amplicon was generated. Multiplex amplification also simplifies the process and reduces costs. [0003] The existing nucleic acid amplification technology mainly includes: heating t...

AI Technical Summary

Problems solved by technology

[0004] The existing nucleic acid amplification instrument is not highly intelligent. In the process of nucleic acid amplification, the addition of primers, buffers, and dNTPs and other components requires manual addition by staff; at the same time, in the process of nucleic acid amplification, often need To amplify nucleic acid multiple times, there are requirements for temperature control in each nucleic acid amplification process, and these operations need to be performed manually, which is time-consuming and laborious, which is not conducive to production and research; therefore, the present invention provides a digital nucleic acid A regulation method of an amplification instrument, to at least partially solve the above-mentioned problems

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